Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 485,860 C→A L34M (CTG→ATG)  acrR → DNA‑binding transcriptional repressor AcrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913485,8600CA99.5% 623.8 / ‑4.1 181L34M (CTG→ATG) acrRDNA‑binding transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (100/80);  total (100/81)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 185 total aligned reads displayed.

TGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGG  >  NC_000913/485725‑485995
                                                                                                                                       |                                                                                                                                       
tgtatgtaAATCTAACGCCTGTAAATTCATGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATggg                                                                                                                                     >  1:1903336/1‑140 (MQ=255)
tgtatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATggg                                                                                                                                     >  2:1557032/1‑140 (MQ=255)
tgtatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATggg                                                                                                                                     <  1:413790/140‑1 (MQ=255)
tgtatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATggg                                                                                                                                     <  1:1418754/140‑1 (MQ=255)
 gtatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGc                                                                                                                                    >  2:1346330/1‑140 (MQ=255)
    tgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCgag                                                                                                                                 >  1:301276/1‑140 (MQ=255)
    tgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCgag                                                                                                                                 >  1:485486/1‑140 (MQ=255)
     gtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCgaga                                                                                                                                >  1:1300208/1‑140 (MQ=255)
       aaaTCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATG‑GGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTg                                                                                                                             <  2:237274/140‑1 (MQ=255)
         aTCTAACGCCTATAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGc                                                                                                                            >  1:819381/1‑140 (MQ=255)
           cTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCaa                                                                                                                          >  2:220406/1‑140 (MQ=255)
           cTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCaa                                                                                                                          >  1:1631884/1‑140 (MQ=255)
              aCGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAag                                                                                                                       >  2:545596/1‑140 (MQ=255)
                gCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAagca                                                                                                                     >  1:1038173/1‑140 (MQ=255)
                gCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAagca                                                                                                                     >  1:215501/1‑140 (MQ=255)
                   tGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCt                                                                                                                  >  2:2088632/1‑140 (MQ=255)
                    gTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTg                                                                                                                 >  1:648394/1‑140 (MQ=255)
                     tAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTgg                                                                                                                >  1:709381/1‑140 (MQ=255)
                     tAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTgg                                                                                                                >  1:1052934/1‑140 (MQ=255)
                     tAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTAGATGGGCGAGATTGCAAAAGCAGCTgg                                                                                                                >  1:2107792/1‑140 (MQ=255)
                             cGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgc                                                                                                        <  2:1944787/140‑1 (MQ=255)
                              gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                                       <  1:1447193/140‑1 (MQ=255)
                              gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                                       <  1:617370/140‑1 (MQ=255)
                              gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                                       <  1:383793/140‑1 (MQ=255)
                              gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                                       <  1:1549069/140‑1 (MQ=255)
                              gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                                       <  2:1052934/140‑1 (MQ=255)
                              gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                                       <  1:1091601/140‑1 (MQ=255)
                               aaCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc                                                                                                      >  1:1958632/1‑140 (MQ=255)
                                aCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgcg                                                                                                     <  1:1988942/140‑1 (MQ=255)
                                  atatGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGt                                                                                                   <  2:1198627/140‑1 (MQ=255)
                                   tatGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTg                                                                                                  <  1:1857096/140‑1 (MQ=255)
                                      ggCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCaa                                                                                               >  2:1475929/1‑140 (MQ=255)
                                      ggCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCaa                                                                                               >  1:552287/1‑140 (MQ=255)
                                           gAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTAc                                                                                          >  1:244480/1‑140 (MQ=255)
                                            aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACt                                                                                         >  2:1993318/1‑140 (MQ=255)
                                            aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACt                                                                                         >  2:1151544/1‑140 (MQ=255)
                                            aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACt                                                                                         >  1:230467/1‑140 (MQ=255)
                                                    aaCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCAtttt                                                                                 <  2:139949/140‑1 (MQ=255)
                                                         gaagCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGa                                                                            >  2:726430/1‑140 (MQ=255)
                                                           agCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACa                                                                          >  2:273355/1‑140 (MQ=255)
                                                           agCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACa                                                                          >  1:113057/1‑140 (MQ=255)
                                                                  gAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGa                                                                   <  1:1639380/140‑1 (MQ=255)
                                                                      cgcgCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTg                                                               <  1:1399786/140‑1 (MQ=255)
                                                                       gcgcCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt                                                              <  2:698209/140‑1 (MQ=255)
                                                                       gcgcCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt                                                              <  1:1837119/140‑1 (MQ=255)
                                                                       gcgcCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt                                                              >  2:1409512/1‑140 (MQ=255)
                                                                       gcgcCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt                                                              <  1:123388/140‑1 (MQ=255)
                                                                            aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                                         <  1:273355/140‑1 (MQ=255)
                                                                            aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                                         <  1:891447/140‑1 (MQ=255)
                                                                            aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                                         <  2:291928/140‑1 (MQ=255)
                                                                            aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                                         <  2:791176/140‑1 (MQ=255)
                                                                            aaCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                                         <  1:1409512/140‑1 (MQ=255)
                                                                             acacATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTg                                                        <  2:1631884/140‑1 (MQ=255)
                                                                              cacaTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTga                                                       <  2:2135847/140‑1 (MQ=255)
                                                                                    cTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTg                                                 <  2:230467/140‑1 (MQ=255)
                                                                                        aTGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAACAGCAGCGGGCGTTACGCGCGGTGCAATCTACTGGCATTGTAAAGACAAGTCGGATTCGTTCAGTTAGATGTGGCaa                                             >  2:1921804/1‑140 (MQ=255)
                                                                                         tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                                         <  1:850237/127‑1 (MQ=255)
                                                                                         tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAAc                                            >  2:982867/1‑140 (MQ=255)
                                                                                         tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAAc                                            <  2:1033078/140‑1 (MQ=255)
                                                                                             gCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTc                                        >  1:733735/1‑140 (MQ=255)
                                                                                             gCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTc                                        >  1:1791710/1‑140 (MQ=255)
                                                                                             gCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTc                                        >  1:1161270/1‑140 (MQ=255)
                                                                                              ctctACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCa                                       >  2:1033338/1‑140 (MQ=255)
                                                                                              ctctACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCa                                       <  1:1151544/140‑1 (MQ=255)
                                                                                              ctctACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCa                                       <  2:1874949/140‑1 (MQ=255)
                                                                                                 tACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGaa                                    <  2:733735/140‑1 (MQ=255)
                                                                                                   cGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCa                                       >  1:1228116/1‑135 (MQ=255)
                                                                                                   cGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCa                                       <  2:1228116/135‑1 (MQ=255)
                                                                                                   cGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATc                                  >  2:940866/1‑140 (MQ=255)
                                                                                                   cGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGAAGCCGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGTCAAGTCGGTTTTGTTCAGTGAGATCTGGGAACTGTCAGAATc                                  >  2:39253/1‑140 (MQ=255)
                                                                                                       ttttCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAat                              >  2:175600/1‑140 (MQ=255)
                                                                                                        tttCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAatt                             >  2:35352/1‑139 (MQ=255)
                                                                                                        tttCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAata                             >  2:1333970/1‑140 (MQ=255)
                                                                                                        tttCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAata                             >  1:1356666/1‑140 (MQ=255)
                                                                                                        tttCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAata                             >  1:1468066/1‑140 (MQ=255)
                                                                                                         ttCTCACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAatat                            <  2:1958632/140‑1 (MQ=255)
                                                                                                           ctcACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTg                          <  2:1279524/140‑1 (MQ=255)
                                                                                                            tcACAGCAGGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCTTTACGCGCGGTACAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTgg                         <  1:1921804/140‑1 (MQ=255)
                                                                                                                 gcagGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAAc                    >  1:734704/1‑140 (MQ=255)
                                                                                                                 gcagGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAAc                    <  2:1654605/140‑1 (MQ=255)
                                                                                                                   agGGGGTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTa                  >  2:367770/1‑140 (MQ=255)
                                                                                                                    gggggTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTag                 <  1:1031797/140‑1 (MQ=255)
                                                                                                                    gggggTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTag                 <  2:851526/140‑1 (MQ=255)
                                                                                                                    gggggTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTACAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTag                 <  2:838656/140‑1 (MQ=255)
                                                                                                                     ggggTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGTTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTaga                >  2:1427723/1‑140 (MQ=255)
                                                                                                                      gggTATCATCCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTagag               >  2:1831518/1‑140 (MQ=255)
                                                                                                                          atcatcCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCttt           >  2:618921/1‑139 (MQ=255)
                                                                                                                          atcatcCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTg           >  1:533914/1‑140 (MQ=255)
                                                                                                                          atcatcCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTg           >  1:1523029/1‑140 (MQ=255)
                                                                                                                          atcatcCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTg           >  1:1722401/1‑140 (MQ=255)
                                                                                                                              tcCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTa       <  1:1161591/140‑1 (MQ=255)
                                                                                                                              tcCACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGCCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTa       >  1:965395/1‑140 (MQ=255)
                                                                                                                               ccACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTAt      <  2:306830/140‑1 (MQ=255)
                                                                                                                                cACCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATc     >  2:577295/1‑140 (MQ=255)
                                                                                                                                 aCCTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCa    >  1:1475965/1‑140 (MQ=255)
                                                                                                                                  ccTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAg   >  2:205063/1‑140 (MQ=255)
                                                                                                                                   cTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCtt            <  2:2069288/130‑1 (MQ=255)
                                                                                                                                   cTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCtt            >  1:2069288/1‑130 (MQ=255)
                                                                                                                                   cTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAgg  >  1:852051/1‑140 (MQ=255)
                                                                                                                                   cTCGATGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGAGTTGTTGAGTGAGATCTGGGTACTGTCAGAATCCAATATTGGTGAAATAGAGCTTGAGTATCAgt  >  1:2091075/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGG  >  NC_000913/485725‑485995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A