Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	643,088	C→A	T334N (ACC→AAC)	ybdR →	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	643,088	0	C	A	100.0%	213.7 / NA	60	T334N (ACC→AAC)	ybdR	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/37); total (23/37)

TAAAGCGTTGCGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGACCCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAA  >  NC_000913/642951‑643221

caaaGCGTTGCGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAacc                                                                                                                                     <  1:1218340/139‑1 (MQ=255)

       ttGCGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCCTTTTTTTCGGACCGGGGGGCTTCGCTGGGTTTTTTCACGGTTTTCTGTTTTGCGGCGCCTTTGTTAAAGGGGTGTCGTTTTAAATGGGACAGAACCACGTTcc                                                                                                                              >  2:330410/1‑139 (MQ=255)

         gCGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACg                                                                                                                            <  2:719750/140‑1 (MQ=255)

          cGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGc                                                                                                                           <  1:1170426/140‑1 (MQ=255)

          cGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGCCTACGCCGGGTTTATTCACGGGTTCCTGTTTGGCGACGCCTTTGCTAAAGGGGTGTCGTTTAAAATGGGACAGAACCACGTTCACGc                                                                                                                           >  1:1245981/1‑140 (MQ=255)

               gtgtATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGc                                                                                                                      <  1:330410/140‑1 (MQ=255)

               gtgtATTGCGGCGGTCAGGCGTAGCGGCATTGTTAGCGTACCGGGCGTCTACTCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGc                                                                                                                      <  1:1256196/140‑1 (MQ=255)

                 gtATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTg                                                                                                                    <  2:1128295/140‑1 (MQ=255)

                   aTTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTggg                                                                                                                  <  1:692406/140‑1 (MQ=255)

                     tGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGgag                                                                                                                <  2:190359/140‑1 (MQ=255)

                     tGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGgag                                                                                                                <  2:313999/140‑1 (MQ=255)

                      gcggcgGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGgaga                                                                                                               <  1:868716/140‑1 (MQ=255)

                         gcggTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAatt                                                                                                            >  1:97695/1‑140 (MQ=255)

                          cggTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAatta                                                                                                           >  1:611464/1‑140 (MQ=255)

                            gTCCGGCGTGGCGGCCTTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGTGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAattatt                                                                                                         >  2:188209/1‑140 (MQ=255)

                            gTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGGTTTATTCACGGTTTTCTGTTTTGCGACGCCTTTGATAAAGGGTTTTCGTTTAAAATGGGGCAGAACCACGTTCCCGCATGGCTGGGGGAattatt                                                                                                         >  2:562713/1‑140 (MQ=255)

                            gTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAattatt                                                                                                         >  1:21477/1‑140 (MQ=255)

                                 gCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGt                                                                                                    <  1:425002/140‑1 (MQ=255)

                                 gCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGt                                                                                                    <  1:337747/140‑1 (MQ=255)

                                  cGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGtt                                                                                                   <  2:687803/140‑1 (MQ=255)

                                    tGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTaa                                                                                                 <  2:611464/140‑1 (MQ=255)

                                     ggcggcATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAAt                                                                                                <  1:1403438/140‑1 (MQ=255)

                                          cATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTgaga                                                                                           <  1:744887/140‑1 (MQ=255)

                                           aTTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGaa                                                                                          >  2:1342070/1‑140 (MQ=255)

                                               ttAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGa                                                                                      <  2:235296/140‑1 (MQ=255)

                                               ttAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGa                                                                                      <  2:1105139/140‑1 (MQ=255)

                                               ttAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGa                                                                                      <  1:342066/140‑1 (MQ=255)

                                                    gTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGTGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACt                                                                                 <  1:188209/140‑1 (MQ=255)

                                                      aCCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGa                                                                               >  1:472329/1‑140 (MQ=255)

                                                         gggCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAAc                                                                            >  1:1170907/1‑140 (MQ=255)

                                                         gggCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAAc                                                                            >  1:442807/1‑140 (MQ=255)

                                                         gggCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAAc                                                                            >  1:38790/1‑140 (MQ=255)

                                                          ggCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAAcc                                                                           >  2:58866/1‑140 (MQ=255)

                                                          ggCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAAcc                                                                           >  2:1025476/1‑140 (MQ=255)

                                                          ggCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAAcc                                                                           >  1:344199/1‑140 (MQ=255)

                                                              tCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCagaa                                                                       >  2:310577/1‑140 (MQ=255)

                                                                tACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCagaaga                                                                     <  2:472329/140‑1 (MQ=255)

                                                                  agCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGACTTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGaaa                                                                   <  2:1183264/139‑1 (MQ=255)

                                                                    cTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAAtt                                                                 <  1:1342070/140‑1 (MQ=255)

                                                                     tGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTg                                                                <  2:97695/140‑1 (MQ=255)

                                                                         tttATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAc                                                            <  2:336526/140‑1 (MQ=255)

                                                                         tttATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAc                                                            <  1:1173187/140‑1 (MQ=255)

                                                                          ttATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAcc                                                           <  2:495251/140‑1 (MQ=255)

                                                                          ttATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAcc                                                           <  2:1127292/140‑1 (MQ=255)

                                                                          ttATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAcc                                                           <  1:1140564/140‑1 (MQ=255)

                                                                          ttATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAcc                                                           <  1:310577/140‑1 (MQ=255)

                                                                           tATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTAccc                                                          <  1:812742/140‑1 (MQ=255)

                                                                                            ttGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGaa                                         >  1:724432/1‑140 (MQ=255)

                                                                                                cGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAgg                                     <  2:344199/140‑1 (MQ=255)

                                                                                                 gACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGgc                                    <  2:38790/140‑1 (MQ=255)

                                                                                                     ccTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGgccgcc                                <  2:21477/140‑1 (MQ=255)

                                                                                                     ccTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGACTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAAgccgcc                                >  1:1433974/1‑140 (MQ=255)

                                                                                                      cTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGccc                               <  1:233342/140‑1 (MQ=255)

                                                                                                         tGATAAAGGGTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCggg                            <  2:177508/140‑1 (MQ=255)

                                                                                                                gggTTGTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATga                     >  1:1117672/1‑140 (MQ=255)

                                                                                                                     gTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGAttt                >  2:77787/1‑140 (MQ=255)

                                                                                                                     gTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGAttt                >  2:981943/1‑140 (MQ=255)

                                                                                                                     gTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGAttt                >  2:316492/1‑140 (MQ=255)

                                                                                                                     gTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGAttt                >  2:304709/1‑140 (MQ=255)

                                                                                                                     gTCGTTTAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGAttt                >  2:251566/1‑140 (MQ=255)

                                                                                                                           tAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGaaa          >  1:1088782/1‑140 (MQ=255)

                                                                                                                           tAAAATGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGaaa          >  1:1046403/1‑140 (MQ=255)

                                                                                                                           tAAAATGGGACAGAACCACGGTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGaaa          >  2:132870/1‑140 (MQ=255)

                                                                                                                            aaaaTGGGACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGaaaa         <  2:1088782/140‑1 (MQ=255)

                                                                                                                                   gACAGAACCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGaa  <  2:724432/140‑1 (MQ=255)

TAAAGCGTTGCGTCAGTGTATTGCGGCGGTCAGGCGTGGCGGCATTGTTAGCGTACCGGGCGTCTACGCTGGATTTATTCACGGTTTCCTGTTTGGCGACGCCTTTGATAAAGGGTTGTCGTTTAAAATGGGACAGACCCACGTTCACGCATGGCTGGGAGAATTATTACCGTTAATTGAGAAAGGATTACTGAAACCAGAAGAAATTGTTACCCACTATATGCCGTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAA  >  NC_000913/642951‑643221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A