Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F48 I1 R1 115 87.5 3003888 99.1% 2976853 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 845,933 C→A K177N (AAG→AAT)  glnQ ← L‑glutamine ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913845,9330CA100.0% 263.7 / NA 74K177N (AAG→AAT) glnQL‑glutamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/38);  total (36/38)

ACACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGT  >  NC_000913/845797‑846066
                                                                                                                                        |                                                                                                                                     
acacCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTc                                                                                                                                    >  1:65344/1‑140 (MQ=255)
 cacCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCa                                                                                                                                   >  1:766083/1‑140 (MQ=255)
 cacCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCa                                                                                                                                   >  1:773910/1‑140 (MQ=255)
  acCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAg                                                                                                                                  <  2:1058903/140‑1 (MQ=255)
     tGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCAc                                                                                                                               >  2:134470/1‑140 (MQ=255)
      gCGGATTGCCATCTTCCGCAATCCGGCGCTTGTCGATAAAGATCGGCCGCTAATCTACTTTCTCTGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACt                                                                                                                              >  1:1411749/1‑140 (MQ=255)
      gCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACt                                                                                                                              >  2:406818/1‑140 (MQ=255)
      gCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACt                                                                                                                              >  2:344319/1‑140 (MQ=255)
       cGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACtt                                                                                                                             >  1:1497719/1‑140 (MQ=255)
       cGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACtt                                                                                                                             >  1:367370/1‑140 (MQ=255)
       cGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACtt                                                                                                                             >  2:1296311/1‑140 (MQ=255)
         gATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCa                                                                                                                           <  2:367370/140‑1 (MQ=255)
         gATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCa                                                                                                                           <  2:87198/140‑1 (MQ=255)
         gATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCa                                                                                                                           >  1:947531/1‑140 (MQ=255)
           ttGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATg                                                                                                                         >  1:335790/1‑140 (MQ=255)
            tGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATgg                                                                                                                        >  1:50171/1‑140 (MQ=255)
            tGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATgg                                                                                                                        >  2:655922/1‑140 (MQ=255)
              ccATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGgcg                                                                                                                      <  2:890961/140‑1 (MQ=255)
              ccATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGgcg                                                                                                                      <  2:237830/140‑1 (MQ=255)
                 tCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAg                                                                                                                   >  1:42062/1‑140 (MQ=255)
                    tCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTc                                                                                                                <  2:766083/140‑1 (MQ=255)
                          aTCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTc                                                                                                          <  1:1282858/140‑1 (MQ=255)
                          aTCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTc                                                                                                          >  1:29949/1‑140 (MQ=255)
                          aTCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTc                                                                                                          <  1:344319/140‑1 (MQ=255)
                           tCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCa                                                                                                         <  1:406818/140‑1 (MQ=255)
                               gCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAagag                                                                                                     >  1:415398/1‑140 (MQ=255)
                                     gTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGaat                                                                                               >  2:399285/1‑139 (MQ=255)
                                     gTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAg                                                                                               >  2:1143587/1‑140 (MQ=255)
                                      tCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGt                                                                                              <  2:415398/140‑1 (MQ=255)
                                      tCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGt                                                                                              <  2:1236886/140‑1 (MQ=255)
                                      tCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGt                                                                                              <  1:489533/140‑1 (MQ=255)
                                      tCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGt                                                                                              <  2:947531/140‑1 (MQ=255)
                                         aTAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGTGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCgg                                                                                           >  2:657410/1‑140 (MQ=255)
                                          tAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGt                                                                                          <  1:618631/140‑1 (MQ=255)
                                          tAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGt                                                                                          >  1:1383836/1‑140 (MQ=255)
                                                 cAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCaa                                                                                   <  1:1296311/140‑1 (MQ=255)
                                                    ccGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACa                                                                                >  1:252814/1‑140 (MQ=255)
                                                    ccGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACa                                                                                >  2:1281652/1‑140 (MQ=255)
                                                       ggAAGCTACTTTCTCGGCAAAACCGATTTCGGGGGCCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGTAAGTCGGTTCATCAAACAGca                                                                             >  2:155632/2‑140 (MQ=255)
                                                       cgAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGca                                                                             <  2:252814/140‑1 (MQ=255)
                                                       cgAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGca                                                                             <  1:1200808/140‑1 (MQ=255)
                                                            cTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCAtt                                                                        <  1:134470/140‑1 (MQ=255)
                                                              aCTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCAtttt                                                                      <  2:980174/140‑1 (MQ=255)
                                                                tttCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCg                                                                    <  2:773910/140‑1 (MQ=255)
                                                                           aaCCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGcc                                                         >  1:202474/1‑140 (MQ=255)
                                                                                        ggTCACGATCCCCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCaa                                            <  1:324607/140‑1 (MQ=255)
                                                                                          tCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATc                                          >  2:678007/1‑140 (MQ=255)
                                                                                              gATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCca                                      >  1:1458469/1‑140 (MQ=255)
                                                                                              aaTCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCca                                      <  2:885141/139‑1 (MQ=255)
                                                                                                tCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCcaca                                    <  1:1240597/140‑1 (MQ=255)
                                                                                                tCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCcaca                                    <  2:202474/140‑1 (MQ=255)
                                                                                                  aCCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACg                                  <  1:629619/140‑1 (MQ=255)
                                                                                                   ccATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACgc                                 <  2:335790/140‑1 (MQ=255)
                                                                                                   acATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACgc                                 <  2:65344/139‑1 (MQ=255)
                                                                                                         tCATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGt                           >  1:430872/1‑140 (MQ=255)
                                                                                                          cATCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGtt                          >  2:1333091/1‑140 (MQ=255)
                                                                                                            tCCCTTCTTCAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGa                        >  1:1447999/1‑140 (MQ=255)
                                                                                                              ccTTCTTCAGCCAGATCCTGCATAACATGCAGCACTGCATGGCGCAGTTCCAGGTCAAGAGAGGGAGTCGGTTCAGCAAACAGCATCATTTTCGGCTTCACCGGCAGCGCGCGGGCAATCGCCACAAGCGGCTATTGacc                      >  1:1411813/1‑140 (MQ=255)
                                                                                                                 tcttcAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGaccacc                   >  2:364600/1‑140 (MQ=255)
                                                                                                                   ttcAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAg                 >  1:956737/1‑140 (MQ=255)
                                                                                                                     cAGCCAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGaa               <  2:747556/140‑1 (MQ=255)
                                                                                                                        ccAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGt            >  2:1163911/1‑140 (MQ=255)
                                                                                                                         cAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGtt           <  1:1125410/140‑1 (MQ=255)
                                                                                                                         cAGATCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGtt           <  1:1247896/140‑1 (MQ=255)
                                                                                                                            aTCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCgg        <  2:29949/140‑1 (MQ=255)
                                                                                                                            aTCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCgg        <  2:265019/140‑1 (MQ=255)
                                                                                                                            aTCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCgg        <  2:1383836/140‑1 (MQ=255)
                                                                                                                            aTCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCgg        >  1:92582/1‑140 (MQ=255)
                                                                                                                            aTCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCgg        <  1:678007/140‑1 (MQ=255)
                                                                                                                            aTCCTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTAATAAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCgg        <  1:155632/140‑1 (MQ=255)
                                                                                                                              ccTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGaa      <  1:399285/140‑1 (MQ=255)
                                                                                                                              ccTGCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGaa      <  1:1143587/140‑1 (MQ=255)
                                                                                                                                 gCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCGCCAGAAAGTTCGGAAggg   <  2:956737/140‑1 (MQ=255)
                                                                                                                                 gCATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGATGACCACCAGAAAGTTCGGAAggg   <  1:1333091/140‑1 (MQ=255)
                                                                                                                                  cATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGt  >  1:342779/1‑140 (MQ=255)
                                                                                                                                  cATAACATTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGt  >  1:1064569/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
ACACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTCACGATCACCATCGTCATCCCTTCTTCAGCCAGATCCTGCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGT  >  NC_000913/845797‑846066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A