Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F48 I1 R1	45	83.3	2696702	99.2%	2675128	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	849,358	G→T	A247E (GCG→GAG)	rhtA ←	L‑threonine/L‑homoserine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	849,358	0	G	T	100.0%	437.2 / NA	120	A247E (GCG→GAG)	rhtA	L‑threonine/L‑homoserine exporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (58/62); total (58/62)

Only 100 of 122 total aligned reads displayed.

TAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAG  >  NC_000913/849222‑849492

tAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCg                                                                                                                                     >  1:159408/1‑140 (MQ=255)

   ttCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGtt                                                                                                                                  <  1:1312366/140‑1 (MQ=255)

   ttCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGtt                                                                                                                                  <  2:927893/140‑1 (MQ=255)

     cTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTcc                                                                                                                                <  1:62078/140‑1 (MQ=255)

      ttttATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTcct                                                                                                                               >  2:168962/1‑139 (MQ=255)

       tttATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCAt                                                                                                                              >  1:1103338/1‑140 (MQ=255)

        ttATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCa                                                                                                                               >  1:185927/1‑138 (MQ=255)

        ttATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCa                                                                                                                               <  2:185927/138‑1 (MQ=255)

        ttATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCAtt                                                                                                                             >  2:552850/1‑139 (MQ=255)

        ttATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATg                                                                                                                             >  1:996111/1‑140 (MQ=255)

               gCTCTCTTTGCGTACTGTCAGCGTAGGCCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCtcatca                                                                                                                      >  2:942564/1‑140 (MQ=255)

                ctctctTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAg                                                                                                                     <  1:168962/140‑1 (MQ=255)

                ctctctTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAg                                                                                                                     <  1:75325/140‑1 (MQ=255)

                atctctTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGCGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCtcatcaa                                                                                                                     >  2:399375/2‑139 (MQ=255)

                     tttGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTAc                                                                                                                >  2:183071/1‑140 (MQ=255)

                     tttGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTAc                                                                                                                >  2:136103/1‑140 (MQ=255)

                      ttGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTAcc                                                                                                               <  2:1221430/140‑1 (MQ=255)

                             cTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCGGCGTACCAAATGTc                                                                                                        >  2:462752/1‑140 (MQ=255)

                             cTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTc                                                                                                        >  2:96294/1‑140 (MQ=255)

                             cTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTc                                                                                                        >  1:142961/1‑140 (MQ=255)

                             cTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTc                                                                                                        >  1:270953/1‑140 (MQ=255)

                             cTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTc                                                                                                        >  1:1106147/1‑140 (MQ=255)

                               gTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCg                                                                                                      >  2:595850/1‑140 (MQ=255)

                               gTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCg                                                                                                      >  1:189986/1‑140 (MQ=255)

                                  aGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCgtgt                                                                                                   <  1:96294/140‑1 (MQ=255)

                                        gACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCaa                                                                                             >  2:219847/1‑140 (MQ=255)

                                        gACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCaa                                                                                             >  1:412203/1‑140 (MQ=255)

                                        gACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCaa                                                                                             >  2:750430/1‑140 (MQ=255)

                                         aCCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCGGCGTACCAAATGTCCGTGTTGGCaaa                                                                                            <  1:462752/140‑1 (MQ=255)

                                         aCCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCaaa                                                                                            <  1:759523/140‑1 (MQ=255)

                                          ccccATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAAc                                                                                           >  1:126993/1‑140 (MQ=255)

                                              aTTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                                                                                       <  2:159408/140‑1 (MQ=255)

                                              aTTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                                                                                       <  1:1107068/140‑1 (MQ=255)

                                              aTTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                                                                                       >  2:1222133/1‑140 (MQ=255)

                                              aTTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGt                                                                                       >  2:1107068/1‑140 (MQ=255)

                                                 gAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAg                                                                                    <  2:1025006/140‑1 (MQ=255)

                                                     cggcgATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCa                                                                                >  1:1247972/1‑140 (MQ=255)

                                                     cggcgATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCa                                                                                >  1:394114/1‑140 (MQ=255)

                                                       gcgATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAAt                                                                              >  2:375911/1‑140 (MQ=255)

                                                       gcgATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAAt                                                                              >  1:1078675/1‑140 (MQ=255)

                                                          atgatAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCAt                                                                           <  2:870506/140‑1 (MQ=255)

                                                             atAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTc                                                                        >  1:25786/1‑140 (MQ=255)

                                                               aGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCa                                                                      <  2:25786/140‑1 (MQ=255)

                                                               aGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCa                                                                      <  1:595850/140‑1 (MQ=255)

                                                               aGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAACCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCa                                                                      <  1:939220/140‑1 (MQ=255)

                                                                 cgcCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCGGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGc                                                                    >  2:679919/1‑140 (MQ=255)

                                                                 cgcCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGc                                                                    >  2:135859/1‑140 (MQ=255)

                                                                    cGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGaa                                                                 <  2:1078675/140‑1 (MQ=255)

                                                                      aGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATa                                                               >  1:803235/1‑140 (MQ=255)

                                                                      aGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATa                                                               >  1:610150/1‑140 (MQ=255)

                                                                      aGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATa                                                               >  1:893428/1‑140 (MQ=255)

                                                                       gcgcCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATaa                                                              >  2:276394/1‑140 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  2:1103338/140‑1 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  1:1051651/140‑1 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  2:126993/140‑1 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  1:136103/140‑1 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  1:219847/140‑1 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  2:610150/140‑1 (MQ=255)

                                                                              tAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCagag                                                       <  2:412203/140‑1 (MQ=255)

                                                                                  tGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGt                                                   <  2:803235/140‑1 (MQ=255)

                                                                                    gaTGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCg                                                 <  1:399375/139‑1 (MQ=255)

                                                                                        ggTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag                                             >  2:21822/1‑140 (MQ=255)

                                                                                         gtgtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagaga                                            >  1:393621/1‑140 (MQ=255)

                                                                                          tgtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGaa                                           >  1:338904/1‑140 (MQ=255)

                                                                                          tgtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGaa                                           >  1:809868/1‑140 (MQ=255)

                                                                                           gtCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCATTCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAAt                                          >  2:225761/1‑140 (MQ=255)

                                                                                            tCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGaa                                           >  2:1219282/1‑138 (MQ=255)

                                                                                            tCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGaa                                           <  1:1219282/138‑1 (MQ=255)

                                                                                            tCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATa                                         >  1:1030832/1‑140 (MQ=255)

                                                                                            tCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATa                                         >  2:922973/1‑140 (MQ=255)

                                                                                              aGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGc                                       >  1:194760/1‑140 (MQ=255)

                                                                                              aGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGc                                       >  1:1148754/1‑140 (MQ=255)

                                                                                              aGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGc                                       >  1:1208301/1‑140 (MQ=255)

                                                                                                   ttCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAg                                  <  1:1147650/140‑1 (MQ=255)

                                                                                                   ttCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAg                                  <  1:585860/140‑1 (MQ=255)

                                                                                                     ctcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGcc                                >  1:1007491/1‑140 (MQ=255)

                                                                                                      tcCGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCa                               <  2:1148754/140‑1 (MQ=255)

                                                                                                        cGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGa                             <  2:887207/140‑1 (MQ=255)

                                                                                                        cGAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGa                             <  2:194760/140‑1 (MQ=255)

                                                                                                         gAGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAc                            <  2:1208301/140‑1 (MQ=255)

                                                                                                          aGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAcc                           <  2:410905/140‑1 (MQ=255)

                                                                                                          aGGAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAcc                           >  1:1265443/1‑140 (MQ=255)

                                                                                                           ggAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAccc                          <  1:276394/140‑1 (MQ=255)

                                                                                                           ggAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAccc                          <  2:1106147/140‑1 (MQ=255)

                                                                                                           ggAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAccc                          <  1:922973/140‑1 (MQ=255)

                                                                                                            gAAAATCATCCCGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCa                         <  1:910410/140‑1 (MQ=255)

                                                                                                                      ccGGAAACGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAAc               >  2:841080/1‑140 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTTCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGa                             >  2:1326856/1‑122 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTTCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGa                             <  1:1326856/122‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACTGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  2:809868/140‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  1:21822/140‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  2:338904/140‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  1:1069447/140‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           >  1:1318642/1‑140 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  1:76605/140‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  2:1318642/140‑1 (MQ=255)

                                                                                                                          aaaCGGCAGCCAGCTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAc           <  1:1203680/140‑1 (MQ=255)

                                                                                                                                   gcagcTCCGGTTACATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCag  <  2:30355/139‑1 (MQ=255)

                                                                                                                                   ccagcTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCag  <  2:1007491/140‑1 (MQ=255)

                                                                                                                                   ccagcTCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCag  <  2:1168496/140‑1 (MQ=255)

TAATTCTTTTATTTTGCTCTCTTTGCGTACTGTCAGCGTAGACCCCATTGAAGCGGCGATGATAGCGCCGAGCGCCAGTAGCTGTATGGGTGTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAG  >  NC_000913/849222‑849492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A