Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F48 I1 R1 115 87.5 3003888 99.1% 2976853 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 997,277 G→T A79D (GCC→GAC)  ssuE ← NADPH‑dependent FMN reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913997,2770GT98.5% 231.0 / ‑2.7 68A79D (GCC→GAC) ssuENADPH‑dependent FMN reductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (26/41);  total (26/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

AAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC  >  NC_000913/997140‑997413
                                                                                                                                         |                                                                                                                                        
aaaCTGGTATAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCGCTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCGCCGGAATAGGCGTCt                                                                                                                                        <  1:675936/140‑1 (MQ=255)
  aCTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCttt                                                                                                                                      <  1:744954/140‑1 (MQ=255)
   atGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTta                                                                                                                                     <  2:539276/139‑1 (MQ=255)
      gTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGCCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATac                                                                                                                                  >  1:1184525/1‑140 (MQ=255)
       tttAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATaca                                                                                                                                 <  2:291496/140‑1 (MQ=255)
                 tAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGc                                                                                                                       <  1:1170673/140‑1 (MQ=255)
                 tAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGc                                                                                                                       >  1:699159/1‑140 (MQ=255)
                  aaTCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCg                                                                                                                      <  2:1184525/140‑1 (MQ=255)
                       acagCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTTGCGACAAt                                                                                                                 >  2:576882/4‑140 (MQ=255)
                        ccGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATc                                                                                                                >  2:955483/1‑140 (MQ=255)
                        ccGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATc                                                                                                                >  1:741715/1‑140 (MQ=255)
                        ccGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATc                                                                                                                >  2:355670/1‑140 (MQ=255)
                         cGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCa                                                                                                               <  1:349866/140‑1 (MQ=255)
                           ccAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGc                                                                                                             >  2:166519/1‑140 (MQ=255)
                              gcagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGccct                                                                                                          >  2:199655/1‑139 (MQ=255)
                               cagATGGGCCACGGTACCGGCCGTCGCCAGCGGTAGCACCAATTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCAt                                                                                                         <  1:711497/140‑1 (MQ=255)
                               cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCAt                                                                                                         <  1:296955/140‑1 (MQ=255)
                               cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCAt                                                                                                         <  2:802086/140‑1 (MQ=255)
                               cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCAt                                                                                                         <  2:1375453/140‑1 (MQ=255)
                               cagATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCAt                                                                                                         <  1:860655/140‑1 (MQ=255)
                                  aTGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCgg                                                                                                      <  1:873219/140‑1 (MQ=255)
                                  aTGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCgg                                                                                                      <  1:1438861/140‑1 (MQ=255)
                                   tGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCTCCGGAATAGGCGTCTTTATACACAGGCGTTGCGACAATCAGCCCATCGGc                                                                                                     <  1:576882/140‑1 (MQ=255)
                                   tGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGc                                                                                                     <  2:231187/140‑1 (MQ=255)
                                   tGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGc                                                                                                     <  2:741715/140‑1 (MQ=255)
                                    gggCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGcc                                                                                                    <  1:1030291/140‑1 (MQ=255)
                                     ggCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGTCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCt                                                                                                   >  2:880206/1‑140 (MQ=255)
                                      gCCACGGTATCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTg                                                                                                  >  2:968179/1‑140 (MQ=255)
                                      gCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGCCAATCAGCCCATCGGCCTg                                                                                                  >  1:1199745/1‑140 (MQ=255)
                                      gCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCtt                                                                                                  >  2:1144355/1‑139 (MQ=255)
                                      gCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTg                                                                                                  >  1:356800/1‑140 (MQ=255)
                                          cGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCGGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGc                                                                                              >  1:667302/1‑140 (MQ=255)
                                          cGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGc                                                                                              >  2:441447/1‑140 (MQ=255)
                                            gTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAg                                                                                            <  1:431679/140‑1 (MQ=255)
                                                      tCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTg                                                                                  <  2:1461596/140‑1 (MQ=255)
                                                      gcGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTg                                                                                  <  2:81058/139‑1 (MQ=255)
                                                       cGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGa                                                                                 >  2:836844/1‑140 (MQ=255)
                                                       cGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGa                                                                                 >  2:146703/1‑140 (MQ=255)
                                                       cGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGa                                                                                 >  1:605880/1‑140 (MQ=255)
                                                       cGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGATCGGTGa                                                                                 <  1:955483/140‑1 (MQ=255)
                                                              ggTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCtt                                                                          <  1:1078716/140‑1 (MQ=255)
                                                              ggTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCtt                                                                          <  1:544140/140‑1 (MQ=255)
                                                                  gCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGt                                                                      >  1:431882/1‑140 (MQ=255)
                                                                  gCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGt                                                                      <  2:699159/140‑1 (MQ=255)
                                                                   caccacTTTGTCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTg                                                                     <  1:880206/140‑1 (MQ=255)
                                                                      cacTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCg                                                                  <  1:166519/140‑1 (MQ=255)
                                                                        cTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGa                                                                <  1:45415/140‑1 (MQ=255)
                                                                          ttGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACt                                                              >  1:716602/1‑140 (MQ=255)
                                                                                tGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGaa                                                        >  1:102535/1‑140 (MQ=255)
                                                                                 gCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGaaa                                                       <  1:146703/140‑1 (MQ=255)
                                                                                      gcgcGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGc                                                  <  2:1199745/140‑1 (MQ=255)
                                                                                      gcgcGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTCGAGTGCCGGACTATCGAAACGAGc                                                  <  2:356800/140‑1 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGa                                                                >  1:935480/1‑125 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGa                                                                <  2:935480/125‑1 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCa                                                 <  2:102535/140‑1 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCa                                                 <  2:867673/140‑1 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCa                                                 <  2:716602/140‑1 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCa                                                 >  1:1170146/1‑140 (MQ=255)
                                                                                       cgcgTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCa                                                 <  2:605880/140‑1 (MQ=255)
                                                                                          gTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaa                                              <  2:1218629/140‑1 (MQ=255)
                                                                                            tCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATaaat                                            >  2:528939/1‑139 (MQ=255)
                                                                                                    cagGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCtt                                    >  2:676554/1‑140 (MQ=255)
                                                                                                            gCAGGGTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCg                            <  1:939759/140‑1 (MQ=255)
                                                                                                               gggTTTTCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAg                         >  1:1081417/1‑140 (MQ=255)
                                                                                                                    ttCAACGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTg                    <  1:355670/140‑1 (MQ=255)
                                                                                                                       aaCGCACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAg                 <  1:676554/140‑1 (MQ=255)
                                                                                                                           cACCGGAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTc             >  1:429180/1‑140 (MQ=255)
                                                                                                                                gAATAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGACTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTg        >  1:301568/1‑140 (MQ=255)
                                                                                                                                   tAGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATa     <  1:1144355/140‑1 (MQ=255)
                                                                                                                                    aGGCGTCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaa    <  1:1002319/140‑1 (MQ=255)
                                                                                                                                     ggcgtcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaaa   <  2:658225/140‑1 (MQ=255)
                                                                                                                                     ggcgtcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaaa   <  2:301568/140‑1 (MQ=255)
                                                                                                                                     ggcgtcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATaaa   <  2:979835/140‑1 (MQ=255)
                                                                                                                                      gcgtcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc  <  2:65091/140‑1 (MQ=255)
                                                                                                                                      gcgtcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc  >  2:471955/1‑140 (MQ=255)
                                                                                                                                      gcgtcTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAc  >  1:355810/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AAACTGGTTTAAGGGCATAATCGACCGCCAGCAGATGGGCCACGGTACCGCCCGTCGCCAGCGGTAGCACCACTTTGCCTTGCAAAGCGCGTTCTGGCAGCAGGTCGAGCAGGGTTTTCAACGCACCGGAATAGGCGGCTTTATACACAGGCGTGGCGACAATCAGCCCATCGGCCTGTTGCAGCTGTTCGGTGAAGGTCTTGAGTGCCGGACTATCGAAACGAGCATAAAGTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAAC  >  NC_000913/997140‑997413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A