Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	102,290	C→A	L20M (CTG→ATG)	ddlB →	D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	102,290	0	C	A	100.0%	307.0 / NA	88	L20M (CTG→ATG)	ddlB	D‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (47/41); total (47/41)

GGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTACACGGTC  >  NC_000913/102154‑102425

ggggCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGa                                                                                                                                      >  1:1255150/1‑140 (MQ=255)

    cTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTc                                                                                                                                  >  1:1923641/1‑140 (MQ=255)

    cTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTc                                                                                                                                  >  1:576671/1‑140 (MQ=255)

        tAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGgc                                                                                                                              <  1:199380/140‑1 (MQ=255)

          atatTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGgcgc                                                                                                                            >  1:519499/1‑140 (MQ=255)

           aatTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCa                                                                                                                           <  2:589133/139‑1 (MQ=255)

            atTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAg                                                                                                                          <  1:170203/140‑1 (MQ=255)

            atTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAg                                                                                                                          <  1:86311/140‑1 (MQ=255)

               ggAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCgg                                                                                                                       >  1:264321/1‑140 (MQ=255)

                 aaaaaTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGgcg                                                                                                                             <  2:710018/132‑1 (MQ=255)

                 aaaaaTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGgcg                                                                                                                             >  1:710018/1‑132 (MQ=255)

                     aTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAg                                                                                                                 >  2:77348/1‑140 (MQ=255)

                     aTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAg                                                                                                                 >  2:381733/1‑140 (MQ=255)

                     aTTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAg                                                                                                                 <  2:519402/140‑1 (MQ=255)

                        gCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCg                                                                                                              <  2:1897393/140‑1 (MQ=255)

                          ccGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGa                                                                                                            <  1:1688353/140‑1 (MQ=255)

                          ccGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGa                                                                                                            <  2:2082717/140‑1 (MQ=255)

                          ccGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGa                                                                                                            <  2:264321/140‑1 (MQ=255)

                           cGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGAc                                                                                                           <  1:893314/140‑1 (MQ=255)

                                  tAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGa                                                                                                    <  2:1902434/140‑1 (MQ=255)

                                     cTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAgg                                                                                                 <  2:859490/140‑1 (MQ=255)

                                       gAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCg                                                                                               <  1:522081/140‑1 (MQ=255)

                                        aaaTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgt                                                                                              >  2:447064/1‑139 (MQ=255)

                                        aaaTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgg                                                                                              >  2:696680/1‑140 (MQ=255)

                                        aaaTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgg                                                                                              >  1:356414/1‑140 (MQ=255)

                                        aaaTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgg                                                                                              >  2:235324/1‑140 (MQ=255)

                                        aaaTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgg                                                                                              >  2:296261/1‑140 (MQ=255)

                                        aaaTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCgg                                                                                              >  1:577185/1‑140 (MQ=255)

                                          aTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTa                                                                                            <  2:1466260/140‑1 (MQ=255)

                                          aTCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTa                                                                                            >  2:1032827/1‑140 (MQ=255)

                                            cAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTAtt                                                                                          <  1:1688746/140‑1 (MQ=255)

                                            cAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTAtt                                                                                          <  1:936964/140‑1 (MQ=255)

                                            cAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTAtt                                                                                          <  1:559456/140‑1 (MQ=255)

                                            aaaaCTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTAtt                                                                                          <  2:1757762/139‑1 (MQ=255)

                                               aCTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGAc                                                                                       <  1:661227/140‑1 (MQ=255)

                                                  gAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGAcgcg                                                                                    >  2:423412/1‑140 (MQ=255)

                                                               ccGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGTCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGAcc                                                                       <  1:146049/140‑1 (MQ=255)

                                                                 tgaggaAGAACAACATGAGTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGATTCTATGAATTCTGGCGCAGCGGGGTTAGCCGGACTGCGTGAAGTCGGTATTGACGCGTATCCTGTCGACCCg                                                                     >  2:2023448/2‑140 (MQ=255)

                                                                 ggaggaAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCg                                                                     >  2:276403/1‑140 (MQ=255)

                                                                   aggaAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGaa                                                                   >  1:1430248/1‑140 (MQ=255)

                                                                        gaaCAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAaagaag                                                              >  2:652954/1‑140 (MQ=255)

                                                                               aTGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGt                                                       >  1:2150934/1‑140 (MQ=255)

                                                                                tGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTg                                                      >  1:817544/1‑140 (MQ=255)

                                                                                tGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTg                                                      >  1:897076/1‑140 (MQ=255)

                                                                                tGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTg                                                      >  1:553258/1‑140 (MQ=255)

                                                                                 gACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGa                                                     <  2:1410322/140‑1 (MQ=255)

                                                                                  aCTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGAc                                                    >  2:2099055/1‑140 (MQ=255)

                                                                                  aCTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGAc                                                    <  1:1086321/140‑1 (MQ=255)

                                                                                   cTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACg                                                   >  2:63297/1‑140 (MQ=255)

                                                                                   cTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACg                                                   >  2:433689/1‑140 (MQ=255)

                                                                                   cTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACg                                                   <  2:436468/140‑1 (MQ=255)

                                                                                   cTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACg                                                   >  1:1181759/1‑140 (MQ=255)

                                                                                   cTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGCATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACg                                                   >  1:300723/1‑140 (MQ=255)

                                                                                    tGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGc                                                  >  1:1315502/1‑140 (MQ=255)

                                                                                        aaaaTCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCATCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACt                                              >  1:2020360/1‑140 (MQ=255)

                                                                                          aaTCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGa                                            <  2:1923641/140‑1 (MQ=255)

                                                                                           aTCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGaa                                           >  1:1789338/1‑140 (MQ=255)

                                                                                           aTCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGATTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGaa                                           >  1:1508090/1‑140 (MQ=255)

                                                                                              gcgGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGTAACTGAAGTc                                        >  1:694839/1‑140 (MQ=255)

                                                                                              gcgGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTc                                        >  2:574662/1‑140 (MQ=255)

                                                                                                ggTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGa                                      <  2:2150934/140‑1 (MQ=255)

                                                                                                ggTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGa                                      <  1:296261/140‑1 (MQ=255)

                                                                                                 gTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGAt                                     <  1:952857/140‑1 (MQ=255)

                                                                                                 gTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGAt                                     >  2:374770/1‑140 (MQ=255)

                                                                                                    cTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATggg                                  <  1:696680/140‑1 (MQ=255)

                                                                                                           gtgggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAg                           <  1:652954/140‑1 (MQ=255)

                                                                                                            tgggACCTCCGCTGAGTGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          >  1:754658/1‑140 (MQ=255)

                                                                                                            tgggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          <  1:63297/140‑1 (MQ=255)

                                                                                                            tgggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          <  2:1430248/140‑1 (MQ=255)

                                                                                                            tgggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          >  1:735889/1‑140 (MQ=255)

                                                                                                            tgggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          >  1:1354610/1‑140 (MQ=255)

                                                                                                             gggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGaa                         <  2:356414/140‑1 (MQ=255)

                                                                                                              ggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCGGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAg                           >  1:1661678/1‑137 (MQ=255)

                                                                                                              ggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCGGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAg                           <  2:1661678/137‑1 (MQ=255)

                                                                                                              ggACCTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGaaa                        >  1:1069891/1‑140 (MQ=255)

                                                                                                                 ccTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          <  1:680705/135‑1 (MQ=255)

                                                                                                                 ccTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGa                          >  2:680705/1‑135 (MQ=255)

                                                                                                                 ccTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAgtg                     >  1:1601545/1‑140 (MQ=255)

                                                                                                                 ccTCCGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAgtg                     >  2:1434434/1‑140 (MQ=255)

                                                                                                                     cGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTa                 <  1:1032827/140‑1 (MQ=255)

                                                                                                                     cGCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTa                 <  2:2041423/140‑1 (MQ=255)

                                                                                                                      gCTGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTAt                <  2:1069891/140‑1 (MQ=255)

                                                                                                                        tGAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATcg              <  2:368754/140‑1 (MQ=255)

                                                                                                                         gAGCGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATcgc             >  1:1059071/1‑140 (MQ=255)

                                                                                                                            cGGGAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCt          >  2:349803/1‑140 (MQ=255)

                                                                                                                             gggAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTa         <  1:433689/140‑1 (MQ=255)

                                                                                                                              ggAAGTTTCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTac        >  2:1714620/1‑140 (MQ=255)

                                                                                                                                   tttCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTACACGGt   <  2:300723/140‑1 (MQ=255)

                                                                                                                                    ttCTATGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTACACGGTc  >  1:1243289/1‑140 (MQ=255)

GGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTGAAGCCGCAAACTCCGGAGGAAGAACAACATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTGAATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTCGACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATCGCGCTACACGGTC  >  NC_000913/102154‑102425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A