Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F48 I1 R1 508 125.6 4303852 99.2% 4269421 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 283,350 C→A A50A (GCC→GCA)  yagF → CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913283,3500CA100.0% 345.4 / NA 101A50A (GCC→GCA) yagFCP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/49);  total (52/49)

Only 100 of 103 total aligned reads displayed.

AAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCA  >  NC_000913/283214‑283484
                                                                                                                                        |                                                                                                                                      
aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAggg                                                                                                                                     <  1:1021571/140‑1 (MQ=255)
aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAggg                                                                                                                                     <  2:1147996/140‑1 (MQ=255)
aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAggg                                                                                                                                     <  1:1741240/140‑1 (MQ=255)
aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAggg                                                                                                                                     <  1:1312255/140‑1 (MQ=255)
aaaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAggg                                                                                                                                     <  1:155311/140‑1 (MQ=255)
 aaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGa                                                                                                                                    >  2:1159711/1‑140 (MQ=255)
 aaTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGa                                                                                                                                    <  2:450523/140‑1 (MQ=255)
  aTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGAt                                                                                                                                   >  1:543144/1‑140 (MQ=255)
  aTTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAAGGAt                                                                                                                                   >  2:2093145/1‑140 (MQ=255)
        aCCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTg                                                                                                                             >  1:388863/1‑140 (MQ=255)
           ccGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGa                                                                                                                          <  1:1132732/140‑1 (MQ=255)
             gCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGAGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACg                                                                                                                        <  2:274117/140‑1 (MQ=255)
                ggACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCca                                                                                                                     <  1:2093145/140‑1 (MQ=255)
                      cgcgTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGGCCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGc                                                                                                               >  2:2009115/1‑140 (MQ=255)
                      cgcgTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGc                                                                                                               >  1:80908/1‑140 (MQ=255)
                      cgcgTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGc                                                                                                               >  2:1236781/1‑140 (MQ=255)
                      cgcgTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGc                                                                                                               >  1:2129800/1‑140 (MQ=255)
                      cgcgTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGc                                                                                                               >  1:431737/1‑140 (MQ=255)
                          ttttATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCAc                                                                                                           <  1:1159711/140‑1 (MQ=255)
                          ttttATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCAc                                                                                                           <  2:2145168/140‑1 (MQ=255)
                           tttATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCAcc                                                                                                          >  1:2143167/1‑140 (MQ=255)
                               tGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCa                                                                                                      <  2:376834/140‑1 (MQ=255)
                                   gTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAaga                                                                                                  >  2:1081896/1‑140 (MQ=255)
                                    ggATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAagag                                                                                                 <  1:1784132/139‑1 (MQ=255)
                                           ccACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGa                                                                                          >  1:1278295/1‑140 (MQ=255)
                                            cACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGAt                                                                                         <  2:388863/140‑1 (MQ=255)
                                            cACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGACAACCTGTTCGGCATGACGCAGAACACAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGAt                                                                                         <  1:732722/140‑1 (MQ=255)
                                              cgcgGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTa                                                                                       >  1:173941/1‑140 (MQ=255)
                                              cgcgGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTa                                                                                       >  1:507113/1‑140 (MQ=255)
                                               gcggcgGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTAt                                                                                      <  1:2009115/140‑1 (MQ=255)
                                                cggcggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTg                                                                                           <  2:1376212/134‑1 (MQ=255)
                                                cggcggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTg                                                                                           >  1:1376212/1‑134 (MQ=255)
                                                cggcggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATc                                                                                     >  1:490728/1‑140 (MQ=255)
                                                cggcggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATc                                                                                     >  2:1952568/1‑140 (MQ=255)
                                                cggcggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATc                                                                                     >  1:2096686/1‑140 (MQ=255)
                                                 ggcggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCg                                                                                    <  1:1081896/140‑1 (MQ=255)
                                                   cggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCCTGACGCAGAACGCAGGGAGGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGCGCTGATTATCGGc                                                                                  >  2:550866/1‑140 (MQ=255)
                                                   cggGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGc                                                                                  >  2:1929761/1‑140 (MQ=255)
                                                       gCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTc                                                                              <  2:2096686/140‑1 (MQ=255)
                                                       gCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTc                                                                              <  2:310149/140‑1 (MQ=255)
                                                       gCCGCAGGGCGCTCTGCCGCCGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGCTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGAGCTGATTATCGGCACTc                                                                              >  2:593115/1‑140 (MQ=255)
                                                         cGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAg                                                                            >  1:1136051/1‑140 (MQ=255)
                                                                cgcTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCa                                                                     >  1:1425190/1‑140 (MQ=255)
                                                                cgcTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTAGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCa                                                                     >  1:1672554/1‑140 (MQ=255)
                                                                  ctctGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATc                                                                   <  1:282181/140‑1 (MQ=255)
                                                                  ctctGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATc                                                                   >  2:625106/1‑140 (MQ=255)
                                                                      gCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCcgcg                                                               <  2:274668/140‑1 (MQ=255)
                                                                      gCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCcgcg                                                               <  2:1632624/140‑1 (MQ=255)
                                                                          cTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCgg                                                           >  2:1434489/1‑140 (MQ=255)
                                                                             aCCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGgaga                                                        >  2:254629/1‑140 (MQ=255)
                                                                               cccGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAgacg                                                      >  1:1951570/1‑140 (MQ=255)
                                                                                ccGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAgacgg                                                     <  1:1629547/140‑1 (MQ=255)
                                                                                 cGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAgacgga                                                    >  1:1345240/1‑140 (MQ=255)
                                                                                 cGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAgacgga                                                    >  2:1520817/1‑140 (MQ=255)
                                                                                 cGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAgacgga                                                    >  2:1758968/1‑140 (MQ=255)
                                                                                         cTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGAGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAAt                                            >  2:790823/1‑140 (MQ=255)
                                                                                            aTGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATcgc                                         <  2:543072/140‑1 (MQ=255)
                                                                                             tGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATcgcg                                        <  1:254629/140‑1 (MQ=255)
                                                                                              ggAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCgcgc                                       >  1:652095/1‑140 (MQ=255)
                                                                                              ggAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCgcgc                                       <  2:543144/140‑1 (MQ=255)
                                                                                                aaTCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTg                                     <  2:1278295/140‑1 (MQ=255)
                                                                                                aaTCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTg                                     <  1:1434489/140‑1 (MQ=255)
                                                                                                 aTCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTgg                                    >  2:1601802/1‑140 (MQ=255)
                                                                                                  tctcCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTggg                                   <  1:1520817/140‑1 (MQ=255)
                                                                                                   ctcCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCGGGAGACGGACGCCCAATCGCGCTGGGc                                  >  1:1340883/1‑140 (MQ=255)
                                                                                                   ctcCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGc                                  >  1:1427016/1‑140 (MQ=255)
                                                                                                     cccAGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTa                                <  1:1929761/140‑1 (MQ=255)
                                                                                                        aGCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGCGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACca                             >  1:1349832/1‑140 (MQ=255)
                                                                                                         gCGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACcac                            >  1:454355/1‑140 (MQ=255)
                                                                                                          cGGCAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACcaca                           >  1:467921/1‑140 (MQ=255)
                                                                                                             cAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCg                        <  2:431737/140‑1 (MQ=255)
                                                                                                             cAACCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCg                        <  2:80908/140‑1 (MQ=255)
                                                                                                             aaaCCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCg                        <  2:618177/139‑1 (MQ=255)
                                                                                                              aaCCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCgg                       >  2:1819208/1‑140 (MQ=255)
                                                                                                               aCCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGTACGCCCAATCGCGCTGGGCTACCACACCggg                      >  2:2148013/1‑140 (MQ=255)
                                                                                                               aCCTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCggg                      >  2:679869/1‑140 (MQ=255)
                                                                                                                ccTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGc                     <  2:1349832/140‑1 (MQ=255)
                                                                                                                ccTGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGc                     >  1:1138603/1‑140 (MQ=255)
                                                                                                                  tGTTCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCAt                   <  2:507113/140‑1 (MQ=255)
                                                                                                                    ttCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTg                 >  1:665395/1‑140 (MQ=255)
                                                                                                                    ttCGGCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTg                 >  1:609914/1‑140 (MQ=255)
                                                                                                                       ggCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGga              <  2:454355/140‑1 (MQ=255)
                                                                                                                        gCATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGgag             <  2:1345240/140‑1 (MQ=255)
                                                                                                                         cATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGgaga            <  2:2143167/140‑1 (MQ=255)
                                                                                                                         cATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGgaga            >  2:691150/1‑140 (MQ=255)
                                                                                                                         cATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGgaga            >  2:2132966/1‑140 (MQ=255)
                                                                                                                         cATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGgaga            >  1:1436892/1‑140 (MQ=255)
                                                                                                                         cATGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGgaga            <  1:625106/140‑1 (MQ=255)
                                                                                                                          aTGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGAt           <  2:173941/140‑1 (MQ=255)
                                                                                                                          aTGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGAt           <  1:2148013/140‑1 (MQ=255)
                                                                                                                          aTGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGAt           <  2:467921/140‑1 (MQ=255)
                                                                                                                          aTGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGAt           <  1:679869/140‑1 (MQ=255)
                                                                                                                          aTGACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGAt           <  2:1425190/140‑1 (MQ=255)
                                                                                                                            gACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCg         <  2:1340883/140‑1 (MQ=255)
                                                                                                                            gACGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCg         <  2:1427016/140‑1 (MQ=255)
                                                                                                                              cGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGTAGATCGGc       >  1:305737/1‑140 (MQ=255)
                                                                                                                              cGCAGAACGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGc       >  1:1894437/1‑140 (MQ=255)
                                                                                                                                   aaCGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCatgca  >  1:496620/1‑140 (MQ=255)
                                                                                                                                   aaCGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCatgca  >  1:697240/1‑140 (MQ=255)
                                                                                                                                   aaCGCAGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCatgca  >  1:1028615/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCGCCGGAGACGGACGCCCAATCGCGCTGGGCTACCACACCGGGCATTGGGAGATCGGCATGCA  >  NC_000913/283214‑283484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A