Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	368,563	C→A	intergenic (‑143/‑48)	mhpR ← / → mhpA	DNA‑binding transcriptional activator MhpR/putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	368,563	0	C	A	99.3%	518.0 / ‑6.3	150	intergenic (‑143/‑48)	mhpR/mhpA	DNA‑binding transcriptional activator MhpR/putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (75/74); minor base T (1/0); total (76/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

GACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGCTGATGA  >  NC_000913/368428‑368698

gACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTa                                                                                                                                     >  2:2143552/1‑140 (MQ=255)

gACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTa                                                                                                                                     >  1:1654885/1‑140 (MQ=255)

      tCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTgc                                                                                                                               >  2:364147/1‑140 (MQ=255)

             ttAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAAc                                                                                                                        <  1:974043/140‑1 (MQ=255)

                 aacaacGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACa                                                                                                                    >  1:1777516/1‑140 (MQ=255)

                      cGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               >  2:1441831/1‑140 (MQ=255)

                      cGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               >  1:260704/1‑140 (MQ=255)

                      cGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               >  1:151161/1‑140 (MQ=255)

                      cGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               >  1:1414749/1‑140 (MQ=255)

                      cGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGATTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               >  2:1747925/1‑140 (MQ=255)

                      cGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGCAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGGTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               >  1:1391708/1‑140 (MQ=255)

                      agtggtgCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTg                                                                                                               <  2:733815/139‑1 (MQ=255)

                       gtggtgCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGt                                                                                                              >  2:204559/1‑140 (MQ=255)

                       gtggtgCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGt                                                                                                              >  2:321733/1‑140 (MQ=255)

                         ggtgCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTcc                                                                                                            <  1:734072/140‑1 (MQ=255)

                             cACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGt                                                                                                        >  1:1631835/1‑140 (MQ=255)

                              aCCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTc                                                                                                       <  1:9048/140‑1 (MQ=255)

                              aCCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTc                                                                                                       >  2:888861/1‑140 (MQ=255)

                               ccTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCg                                                                                                      <  1:717596/140‑1 (MQ=255)

                                 tGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTg                                                                                                    <  2:1656675/140‑1 (MQ=255)

                                 tGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTg                                                                                                    <  1:364147/140‑1 (MQ=255)

                                 tGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTg                                                                                                    <  1:1216629/140‑1 (MQ=255)

                                 tGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTg                                                                                                    <  2:260704/140‑1 (MQ=255)

                                 tGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTg                                                                                                    >  2:1030155/1‑140 (MQ=255)

                                    tGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAgg                                                                                                 <  2:612193/140‑1 (MQ=255)

                                    tGCACATTCGGACATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTcc                                                                                                            <  2:834144/129‑1 (MQ=255)

                                     gCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGt                                                                                                <  1:1343574/140‑1 (MQ=255)

                                     gCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGt                                                                                                <  2:186264/140‑1 (MQ=255)

                                          ttCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGa                                                                                           >  1:1982701/1‑140 (MQ=255)

                                          ttCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGa                                                                                           <  1:1596658/140‑1 (MQ=255)

                                            cGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCACGCAACGACATTTTGTCCGAGTCGTGAGGTACTGaat                                                                                         >  2:1771779/1‑139 (MQ=255)

                                             gggCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAAt                                                                                        <  2:1081768/140‑1 (MQ=255)

                                             gggCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAAt                                                                                        <  1:1465693/140‑1 (MQ=255)

                                              ggCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATg                                                                                       >  1:224784/1‑140 (MQ=255)

                                               gCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATgg                                                                                      <  2:523648/140‑1 (MQ=255)

                                                  tGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCaa                                                                                   >  2:322272/1‑140 (MQ=255)

                                                           tGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACAcc                                                                          <  1:489920/140‑1 (MQ=255)

                                                            gTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACAccc                                                                         <  1:119618/140‑1 (MQ=255)

                                                             tAGCCGAAAACACCCTTCCTGTACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCt                                                                        >  2:1929512/1‑140 (MQ=255)

                                                             tAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCt                                                                        >  1:763018/1‑140 (MQ=255)

                                                              aGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTg                                                                       >  2:146145/1‑140 (MQ=255)

                                                              aGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTg                                                                       >  1:764679/1‑140 (MQ=255)

                                                               gCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGa                                                                      >  1:210174/1‑140 (MQ=255)

                                                                   aaaaCACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATc                                                                  <  1:334477/140‑1 (MQ=255)

                                                                   aaaaCACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATc                                                                  <  1:2143552/140‑1 (MQ=255)

                                                                     aaCACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCa                                                                <  1:321733/140‑1 (MQ=255)

                                                                       cacCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGc                                                              <  1:1252728/140‑1 (MQ=255)

                                                                        acCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTATTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGcc                                                             <  2:725014/140‑1 (MQ=255)

                                                                        acCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGcc                                                             <  2:764679/140‑1 (MQ=255)

                                                                        acCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGcc                                                             <  2:952906/140‑1 (MQ=255)

                                                                        acCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGcc                                                             <  1:1030155/140‑1 (MQ=255)

                                                                        acCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGcc                                                             <  1:1500013/140‑1 (MQ=255)

                                                                          ccTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCctg                                                           <  2:1982701/140‑1 (MQ=255)

                                                                          ccTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCctg                                                           <  2:1631835/140‑1 (MQ=255)

                                                                               atataCTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGtt                                                      <  2:877149/139‑1 (MQ=255)

                                                                                 ataCTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTaa                                                    <  2:1247817/140‑1 (MQ=255)

                                                                                    cTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCa                                                 <  1:1805642/140‑1 (MQ=255)

                                                                                      gAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATa                                               >  2:244654/1‑140 (MQ=255)

                                                                                      gAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATa                                               <  2:482499/140‑1 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  2:2114344/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  2:229305/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  2:1905747/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  2:1873467/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              <  1:204559/140‑1 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:378998/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:188196/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:586045/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:1463869/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:1159472/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:845576/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAg                                              >  1:960004/1‑140 (MQ=255)

                                                                                       aGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCC‑GACATCCAGCCTGCTGTTAACCATAGc                                             >  2:1189939/1‑140 (MQ=255)

                                                                                        gcgcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGc                                             >  2:1260109/1‑140 (MQ=255)

                                                                                        gcgcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGc                                             >  2:1204615/1‑140 (MQ=255)

                                                                                        gcgcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGc                                             >  2:2076267/1‑140 (MQ=255)

                                                                                         cgcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCg                                            >  1:898230/1‑140 (MQ=255)

                                                                                         cgcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCg                                            >  2:1585759/1‑140 (MQ=255)

                                                                                         cgcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGCACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCg                                            >  1:661607/1‑140 (MQ=255)

                                                                                          gcACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGcc                                                             >  1:1901106/1‑122 (MQ=255)

                                                                                           aacaATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGtt                                          <  2:378998/139‑1 (MQ=255)

                                                                                              aaTAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAg                                       <  2:2139636/140‑1 (MQ=255)

                                                                                                    aaaTCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCg                                 <  2:428196/140‑1 (MQ=255)

                                                                                                    aaaTCATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCg                                 <  2:586045/140‑1 (MQ=255)

                                                                                                        cATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCg                             >  1:362323/1‑140 (MQ=255)

                                                                                                        cATTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCg                             >  1:823695/1‑140 (MQ=255)

                                                                                                         aTTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGc                            <  1:2114344/140‑1 (MQ=255)

                                                                                                         aTTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGc                            <  1:2076267/140‑1 (MQ=255)

                                                                                                         aTTTACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGc                            <  1:1585759/140‑1 (MQ=255)

                                                                                                          tttACATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCt                           >  2:1626489/1‑140 (MQ=255)

                                                                                                              cATGTTTTTAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTg                       <  1:1260109/140‑1 (MQ=255)

                                                                                                                  tttttAACAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCgg                   <  2:1159472/140‑1 (MQ=255)

                                                                                                                       aaCAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCgg              >  1:382308/1‑140 (MQ=255)

                                                                                                                       aaCAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCgg              <  2:68096/140‑1 (MQ=255)

                                                                                                                         cAAAATAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGtt            <  1:1009709/140‑1 (MQ=255)

                                                                                                                         aaaaaTAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGtt            <  2:1293906/139‑1 (MQ=255)

                                                                                                                            aaTAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCgg                   <  1:2135985/130‑1 (MQ=255)

                                                                                                                            aaTAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCgg                   >  2:2135985/1‑130 (MQ=255)

                                                                                                                            aaTAAGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTggg         <  2:592706/140‑1 (MQ=255)

                                                                                                                                aGTTGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGCtga     <  2:151161/140‑1 (MQ=255)

                                                                                                                                  ttGCGATGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGCtgatg   >  1:1692036/1‑140 (MQ=255)

GACATTTCTATAGTTAAAACAACGTGGTGCACCTGGTGCACATTCGGGCATGTTTTGATTGTAGCCGAAAACACCCTTCCTATACTGAGCGCACAATAAAAAATCATTTACATGTTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGACATTTTGTCCGAGTCGTGAGGTACTGAAATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGCCCGGTTGGGCTGATGA  >  NC_000913/368428‑368698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A