Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	710,592	G→T	R19S (CGT→AGT)	fur ←	DNA‑binding transcriptional dual regulator Fur

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	710,592	0	G	T	99.4%	611.2 / ‑3.0	179	R19S (CGT→AGT)	fur	DNA‑binding transcriptional dual regulator Fur
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (92/85); total (92/87)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01

Only 100 of 180 total aligned reads displayed.

GTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAT  >  NC_000913/710455‑710725

gtcgtcAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAg                                                                                                                                     >  2:347557/1‑140 (MQ=255)

 tcgtcAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAgg                                                                                                                                    <  1:349906/140‑1 (MQ=255)

  cgtcAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGa                                                                                                                                   >  1:43599/1‑140 (MQ=255)

   gtcAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGaa                                                                                                                                  <  1:292126/140‑1 (MQ=255)

    tcAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAg                                                                                                                                 >  2:2068160/1‑140 (MQ=255)

        aCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGtt                                                                                                                             <  1:1099027/140‑1 (MQ=255)

        aCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGtt                                                                                                                             <  1:6974/140‑1 (MQ=255)

        aCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGtt                                                                                                                             <  1:755026/140‑1 (MQ=255)

        aCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGtt                                                                                                                             <  2:841700/140‑1 (MQ=255)

            gTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACtt                                                                                                                         >  1:1029374/1‑140 (MQ=255)

              tCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTc                                                                                                                       >  1:280172/1‑140 (MQ=255)

                  tACGCGATATTCCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACCAGGAAGCGTTACTTTCAGGc                                                                                                                   <  1:1362190/140‑1 (MQ=255)

                  tACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGc                                                                                                                   <  1:870268/140‑1 (MQ=255)

                  aaCGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGc                                                                                                                   >  2:2019540/2‑140 (MQ=255)

                              cGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTa                                                                                                       <  1:1823698/140‑1 (MQ=255)

                              cGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTa                                                                                                       <  1:492046/140‑1 (MQ=255)

                                 aGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    <  1:1904886/140‑1 (MQ=255)

                                 aGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    <  1:1285802/140‑1 (MQ=255)

                                 aGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    <  1:2019540/140‑1 (MQ=255)

                                 aGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    <  1:468824/140‑1 (MQ=255)

                                 aGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    <  2:1820496/140‑1 (MQ=255)

                                  gCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTa                                                                                                       >  2:1421182/1‑136 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    <  2:521913/138‑1 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAggg                                                                                                    >  1:521913/1‑138 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCg                                                                                                  >  2:676705/1‑140 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCg                                                                                                  >  1:1563668/1‑140 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCg                                                                                                  >  1:1748892/1‑140 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCg                                                                                                  >  2:469393/1‑140 (MQ=255)

                                   ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCACCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCg                                                                                                  >  1:1838786/1‑140 (MQ=255)

                                     aGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGt                                                                                                >  2:1367985/1‑140 (MQ=255)

                                     aGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGt                                                                                                >  1:1876363/1‑140 (MQ=255)

                                     aGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGt                                                                                                >  1:1332373/1‑140 (MQ=255)

                                      gACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTa                                                                                               <  2:1029374/140‑1 (MQ=255)

                                      gACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTa                                                                                               >  2:1246909/1‑140 (MQ=255)

                                       aCCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAt                                                                                              >  2:1654918/1‑140 (MQ=255)

                                       aCCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAt                                                                                              >  2:1125407/1‑140 (MQ=255)

                                       aCCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAt                                                                                              >  2:2052075/1‑140 (MQ=255)

                                       aCCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAt                                                                                              >  2:993809/1‑140 (MQ=255)

                                       aCCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAt                                                                                              >  1:1661883/1‑140 (MQ=255)

                                       aCCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGACTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAt                                                                                              >  2:617892/1‑140 (MQ=255)

                                        ccAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTAtt                                                                                             >  1:1294581/1‑140 (MQ=255)

                                             ttcttcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTAt                                                                                        >  2:1993874/1‑140 (MQ=255)

                                                 tcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGt                                                                                    <  1:2052075/140‑1 (MQ=255)

                                                 tcACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGt                                                                                    <  2:1747670/140‑1 (MQ=255)

                                                     ccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATg                                                                                <  1:2068160/140‑1 (MQ=255)

                                                     ccATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATg                                                                                <  2:1768480/140‑1 (MQ=255)

                                                      cATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGc                                                                               <  1:1241869/140‑1 (MQ=255)

                                                      cATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGc                                                                               >  1:1303303/1‑140 (MQ=255)

                                                       atatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGCATTGTTATCAGTCATGCg                                                                              >  1:1548714/1‑140 (MQ=255)

                                                        tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCgg                                                                             >  1:1928317/1‑140 (MQ=255)

                                                        tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCgg                                                                             >  2:243791/1‑140 (MQ=255)

                                                            gATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATc                                                                         <  2:1661883/140‑1 (MQ=255)

                                                             aTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCt                                                                        <  1:676705/140‑1 (MQ=255)

                                                             aTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCt                                                                        <  1:204776/140‑1 (MQ=255)

                                                              acAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTg                                                                       <  2:758857/139‑1 (MQ=255)

                                                               cAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTCGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGt                                                                      >  1:969086/1‑140 (MQ=255)

                                                                aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                     <  1:1654918/140‑1 (MQ=255)

                                                                aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                     <  1:993809/140‑1 (MQ=255)

                                                                aGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                     <  1:1125407/140‑1 (MQ=255)

                                                                       tGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                     <  1:600789/133‑1 (MQ=255)

                                                                       tGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTc                                                                     >  2:600789/1‑133 (MQ=255)

                                                                        gTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACt                                                             <  1:320667/140‑1 (MQ=255)

                                                                           taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                          <  2:1876363/140‑1 (MQ=255)

                                                                           taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                          <  1:14409/140‑1 (MQ=255)

                                                                           taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                          <  2:280172/140‑1 (MQ=255)

                                                                           taAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                          <  1:1798384/140‑1 (MQ=255)

                                                                            aaaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGc                                                         >  2:879545/1‑140 (MQ=255)

                                                                            aaaTCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGc                                                         >  2:858967/1‑140 (MQ=255)

                                                                                cTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                     <  2:291109/140‑1 (MQ=255)

                                                                                cTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGtt                                                     <  2:1563668/140‑1 (MQ=255)

                                                                                   ccGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTGAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCAc                                                  >  1:903683/1‑140 (MQ=255)

                                                                                   ccGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                          >  1:1152641/1‑132 (MQ=255)

                                                                                   ccGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAg                                                          <  2:1152641/132‑1 (MQ=255)

                                                                                   ccGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCAc                                                  >  1:826844/1‑140 (MQ=255)

                                                                                   ccGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGACCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCAc                                                  >  2:1494202/1‑140 (MQ=255)

                                                                                      cACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTc                                               <  2:1896271/140‑1 (MQ=255)

                                                                                        cTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAt                                             <  2:437077/140‑1 (MQ=255)

                                                                                           aCGTGTTGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTag                                          >  2:2036554/1‑140 (MQ=255)

                                                                                            cGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTaga                                         >  1:1742688/1‑140 (MQ=255)

                                                                                                   gTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                  >  2:340391/1‑140 (MQ=255)

                                                                                                     tGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAc                                <  1:251826/140‑1 (MQ=255)

                                                                                                       tCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAg                              >  1:1601561/1‑140 (MQ=255)

                                                                                                          ggCTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAt                           <  1:77969/140‑1 (MQ=255)

                                                                                                            cTCCTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAttt                         >  1:1489018/1‑140 (MQ=255)

                                                                                                               cTGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAttt                         >  1:768969/1‑137 (MQ=255)

                                                                                                                tGAAGAACTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctc                     <  1:1528417/140‑1 (MQ=255)

                                                                                                                 gaagaaCTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctct                    <  2:1489018/140‑1 (MQ=255)

                                                                                                                  aagaaCTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCtt                   >  2:1825721/1‑140 (MQ=255)

                                                                                                                      aCTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga               <  1:340391/140‑1 (MQ=255)

                                                                                                                      aCTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga               <  1:1032028/140‑1 (MQ=255)

                                                                                                                       cTTCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTATAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga               >  2:109852/1‑139 (MQ=255)

                                                                                                                        ttCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa             <  1:1685240/140‑1 (MQ=255)

                                                                                                                         tCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATaa            >  1:1780145/1‑140 (MQ=255)

                                                                                                                         tCCAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATaa            >  1:1146711/1‑140 (MQ=255)

                                                                                                                           cAGGATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATg          <  2:1308558/140‑1 (MQ=255)

                                                                                                                             ggATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCg        <  2:1601561/140‑1 (MQ=255)

                                                                                                                             ggATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCg        <  1:1650022/140‑1 (MQ=255)

                                                                                                                             ggATTTTTAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCg        <  2:78426/140‑1 (MQ=255)

                                                                                                                                 ttttAAACTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa      >  1:1412981/1‑138 (MQ=255)

                                                                                                                                   ttgaaCTAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAt  <  2:903683/137‑1 (MQ=255)

GTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAT  >  NC_000913/710455‑710725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A