Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	851,280	C→A	D440Y (GAT→TAT)	opgE ←	phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	851,280	0	C	A	99.1%	373.0 / ‑3.1	110	D440Y (GAT→TAT)	opgE	phosphoethanolamine transferase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (48/61); total (48/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 118 total aligned reads displayed.

TTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATCATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCGTC  >  NC_000913/851146‑851416

tttAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATggg                                                                                                                                     >  1:258394/1‑140 (MQ=255)

tttAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATggg                                                                                                                                     <  1:259518/140‑1 (MQ=255)

tttAATCCCCGTCCACTGGGAGAAAAAGACTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATCATggg                                                                                                                                     <  2:904730/140‑1 (MQ=255)

 ttAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGc                                                                                                                                    <  1:1821872/140‑1 (MQ=255)

  tAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCa                                                                                                                                   <  1:2041593/140‑1 (MQ=255)

  tAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCa                                                                                                                                   <  2:1249912/140‑1 (MQ=255)

  tAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCa                                                                                                                                   <  1:1377257/140‑1 (MQ=255)

  tAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCa                                                                                                                                   <  2:1025638/140‑1 (MQ=255)

     tCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAgg                                                                                                                                <  2:1011389/140‑1 (MQ=255)

       cccGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTa                                                                                                                              <  2:1355094/140‑1 (MQ=255)

         cGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGTGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTAtt                                                                                                                            >  2:359896/1‑140 (MQ=255)

          gTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTAttt                                                                                                                           >  2:1211001/1‑139 (MQ=255)

          gTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTg                                                                                                                           <  1:1946757/140‑1 (MQ=255)

          gTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTg                                                                                                                           >  2:932846/1‑140 (MQ=255)

            ccACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCa                                                                                                                         <  2:39478/140‑1 (MQ=255)

            ccACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCa                                                                                                                         <  1:44080/140‑1 (MQ=255)

               cTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATTATAATGGGCAAGGTATTGCACGt                                                                                                                      >  1:1227497/1‑140 (MQ=255)

               cTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGt                                                                                                                      <  1:1934120/140‑1 (MQ=255)

                    agaAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTa                                                                                                                 <  2:2104039/140‑1 (MQ=255)

                    agaAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAAGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCtttt                                                                                                                 >  2:1615203/1‑139 (MQ=255)

                      aaaaaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTAcc                                                                                                               >  1:783870/1‑140 (MQ=255)

                       aaaaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACcg                                                                                                              <  2:1586036/140‑1 (MQ=255)

                       aaaaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACcg                                                                                                              <  1:431311/140‑1 (MQ=255)

                        aaaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACcgc                                                                                                             <  2:258394/140‑1 (MQ=255)

                         aaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACcgcg                                                                                                            <  1:1902129/140‑1 (MQ=255)

                         aaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACcgcg                                                                                                            <  2:1585143/140‑1 (MQ=255)

                         aaGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACcgcg                                                                                                            <  1:694703/140‑1 (MQ=255)

                          aGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCgcgc                                                                                                           <  2:1049328/140‑1 (MQ=255)

                          aGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATACGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCgcgc                                                                                                           <  2:1767945/140‑1 (MQ=255)

                           gCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGct                                                                                                          <  2:1245649/140‑1 (MQ=255)

                              tAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCtt                                                                                                       >  2:986473/1‑140 (MQ=255)

                                    gTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAgg                                                                                                 <  2:506789/140‑1 (MQ=255)

                                      cATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGcc                                                                                               >  1:319648/1‑140 (MQ=255)

                                         tGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGa                                                                                            >  2:522620/1‑140 (MQ=255)

                                            cTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCg                                                                                         >  1:101908/1‑140 (MQ=255)

                                             tGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGt                                                                                        <  2:783870/140‑1 (MQ=255)

                                               aGCTGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTgt                                                                                      >  2:841498/1‑139 (MQ=255)

                                               aGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTgg                                                                                      >  1:482963/1‑140 (MQ=255)

                                               aGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTgg                                                                                      >  1:2015653/1‑140 (MQ=255)

                                               aGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTgg                                                                                      >  1:1270463/1‑140 (MQ=255)

                                                gcggcgGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTg                                                                                       >  2:200552/1‑138 (MQ=255)

                                                gcggcgGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTg                                                                                       <  1:200552/138‑1 (MQ=255)

                                                  ggcggGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCa                                                                                   <  2:70174/140‑1 (MQ=255)

                                                    cggACTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGa                                                                                 >  1:605661/1‑140 (MQ=255)

                                                     gggCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTTATATTTATCATAATGGGCCAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGaa                                                                                >  2:1828060/1‑140 (MQ=255)

                                                         tttAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGt                                                                            <  2:2015653/140‑1 (MQ=255)

                                                             aTCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTCAAc                                                                        <  1:1828060/140‑1 (MQ=255)

                                                             aTCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAAc                                                                        <  2:139774/140‑1 (MQ=255)

                                                                 cacGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGc                                                                    >  1:1347568/1‑140 (MQ=255)

                                                                  acGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCg                                                                   >  2:1382952/1‑140 (MQ=255)

                                                                  acGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATACTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGATAAGTAAACCAGCg                                                                   >  2:953081/1‑140 (MQ=255)

                                                                   cGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCga                                                                  >  1:815549/1‑140 (MQ=255)

                                                                     aTGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCgaga                                                                <  1:1111815/140‑1 (MQ=255)

                                                                      tGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGaa                                                               >  1:94182/1‑140 (MQ=255)

                                                                            ttATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAgctgct                                                         >  1:653502/1‑140 (MQ=255)

                                                                              aTCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCtgctgc                                                       >  1:1721321/1‑140 (MQ=255)

                                                                                cgtcgCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAg                                                              <  2:38314/131‑1 (MQ=255)

                                                                                   cgCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTg                                                  <  1:434550/140‑1 (MQ=255)

                                                                                   cgCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTg                                                  <  2:1270463/140‑1 (MQ=255)

                                                                                   cgCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTg                                                  <  1:685521/140‑1 (MQ=255)

                                                                                   cgCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTg                                                  <  1:707104/140‑1 (MQ=255)

                                                                                     cTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGtt                                                >  2:561791/1‑140 (MQ=255)

                                                                                     cTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGtt                                                >  2:373139/1‑140 (MQ=255)

                                                                                     cTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGtt                                                <  2:1655599/140‑1 (MQ=255)

                                                                                     cTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGtt                                                >  2:714886/1‑140 (MQ=255)

                                                                                          aaTGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTa                                           <  2:1484517/140‑1 (MQ=255)

                                                                                          aaTGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTa                                           <  2:1718443/140‑1 (MQ=255)

                                                                                          aaTGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTa                                           <  1:561791/140‑1 (MQ=255)

                                                                                            tGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTATCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAAc                                         <  1:359896/140‑1 (MQ=255)

                                                                                            agACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAAc                                         <  2:843542/139‑1 (MQ=255)

                                                                                             gACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAGAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACt                                        <  2:1227497/140‑1 (MQ=255)

                                                                                             gACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACt                                        <  2:794711/140‑1 (MQ=255)

                                                                                             gACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACt                                        >  2:1361955/1‑140 (MQ=255)

                                                                                             gACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACt                                        >  2:1797799/1‑140 (MQ=255)

                                                                                             gACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACt                                        <  2:94182/140‑1 (MQ=255)

                                                                                              aCCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTg                                       >  1:1184406/1‑140 (MQ=255)

                                                                                              aCCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTg                                       <  1:1444222/140‑1 (MQ=255)

                                                                                                  aaaaaGGCTCCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCg                                   <  2:815549/139‑1 (MQ=255)

                                                                                                         cACCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCt                            >  1:1479847/1‑140 (MQ=255)

                                                                                                          aCCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCtt                           >  2:183339/1‑140 (MQ=255)

                                                                                                          aCCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCtt                           <  1:2113832/140‑1 (MQ=255)

                                                                                                          aCCTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCtt                           <  1:714886/140‑1 (MQ=255)

                                                                                                           ccTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTg                          >  1:1262754/1‑140 (MQ=255)

                                                                                                            cTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTgc                         >  1:1381816/1‑140 (MQ=255)

                                                                                                            cTGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGCAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTgc                         >  1:881025/1‑140 (MQ=255)

                                                                                                             tGGAAATTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTgcg                        >  2:299052/1‑140 (MQ=255)

                                                                                                                  aTTTTGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTa                   <  1:183339/140‑1 (MQ=255)

                                                                                                                    tttGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTaaa                 <  2:1060721/140‑1 (MQ=255)

                                                                                                                      tGCTGATATTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATc               >  2:421615/1‑140 (MQ=255)

                                                                                                                            tatTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGt         <  1:841498/140‑1 (MQ=255)

                                                                                                                            tatTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGt         <  2:1347568/140‑1 (MQ=255)

                                                                                                                            aatTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGt         <  2:622538/139‑1 (MQ=255)

                                                                                                                             atTTATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGtt        <  2:1721321/140‑1 (MQ=255)

                                                                                                                               ttATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTg      <  2:337083/140‑1 (MQ=255)

                                                                                                                               ttATCATAATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTg      <  1:514785/140‑1 (MQ=255)

                                                                                                                                 atcataATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCg    <  2:1262754/140‑1 (MQ=255)

                                                                                                                                   cataatGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCGTc  <  2:1184406/140‑1 (MQ=255)

                                                                                                                                   cataatGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCGTc  <  1:1361955/140‑1 (MQ=255)

                                                                                                                                   cataatGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCGTc  <  1:1726440/140‑1 (MQ=255)

                                                                                                                                   cataatGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGCGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCGTc  <  1:71740/140‑1 (MQ=255)

TTTAATCCCCGTCCACTGGGAGAAAAAGCCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTTATCGTCGCTGGAAATGACCATAAAAGGCACCTGGAAATTTTGCTGATATTTATCATCATGGGCAAGGTATTGCACGTCTTTACCGCGCTCTTTAAAGGCCAGACCGTGGTCAGAAAAGTAAACCAGCGAGAAGCTGCTGCCGCTGTTGCGTAACTGATCGTACAGCTTGCGCAGTAAATCGTCCGTTTGCGTC  >  NC_000913/851146‑851416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A