Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F48 I1 R1	508	125.6	4303852	99.2%	4269421	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,005,609	G→T	R281L (CGC→CTC)	pyrD →	dihydroorotate dehydrogenase, type 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,005,609	0	G	T	100.0%	378.3 / NA	110	R281L (CGC→CTC)	pyrD	dihydroorotate dehydrogenase, type 2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (66/44); total (66/44)

Only 100 of 110 total aligned reads displayed.

GCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCGCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTA  >  NC_000913/1005474‑1005737

gCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTg                                                                                                                              >  1:258443/1‑140 (MQ=255)

  cATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTc                                                                                                                            >  1:824617/1‑140 (MQ=255)

   ataataTTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCa                                                                                                                           <  2:1491843/140‑1 (MQ=255)

    taataTTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCAc                                                                                                                          <  2:1771081/140‑1 (MQ=255)

    taataTTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCAc                                                                                                                          <  1:1286051/140‑1 (MQ=255)

     aataTTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACt                                                                                                                         <  1:1671502/140‑1 (MQ=255)

      atatTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTg                                                                                                                        <  1:1582471/140‑1 (MQ=255)

            aTGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTa                                                                                                                  >  1:130041/1‑140 (MQ=255)

              ggCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAGAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTaaa                                                                                                                >  1:58360/1‑140 (MQ=255)

              ggCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTaaa                                                                                                                >  2:327503/1‑140 (MQ=255)

              ggCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTaaa                                                                                                                <  2:114627/140‑1 (MQ=255)

               gCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAAc                                                                                                               >  2:589829/1‑140 (MQ=255)

                cGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACg                                                                                                              >  1:609841/1‑140 (MQ=255)

                  ttATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCAc                                                                                                                          >  1:1332122/1‑126 (MQ=255)

                  ttATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGt                                                                                                            <  1:1841854/140‑1 (MQ=255)

                  ttATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGt                                                                                                            <  1:2044167/140‑1 (MQ=255)

                   tATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTc                                                                                                           >  2:1146461/1‑140 (MQ=255)

                   tATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTc                                                                                                           >  1:466628/1‑140 (MQ=255)

                      tGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCt                                                                                                        <  2:514914/140‑1 (MQ=255)

                        cAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTa                                                                                                      <  1:589829/140‑1 (MQ=255)

                            cAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGa                                                                                                  <  2:258443/140‑1 (MQ=255)

                               tACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGatca                                                                                               >  1:934701/1‑140 (MQ=255)

                               tACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGatca                                                                                               >  1:1033580/1‑140 (MQ=255)

                                aCCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGatcat                                                                                              <  2:630815/140‑1 (MQ=255)

                                  cacaCTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCg                                                                                            <  1:910177/140‑1 (MQ=255)

                                   acacTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCgg                                                                                           >  2:1216111/1‑140 (MQ=255)

                                    cacTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGgt                                                                                          >  1:1691547/1‑140 (MQ=255)

                                      cTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGgtgt                                                                                        >  2:1144023/1‑140 (MQ=255)

                                      cTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGgtgt                                                                                        >  1:2082560/1‑140 (MQ=255)

                                      cTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGgtgt                                                                                        >  1:383910/1‑140 (MQ=255)

                                       tCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGtt                                                                                       >  2:459115/1‑140 (MQ=255)

                                         gATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCGCCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTgg                                                                                     <  1:582693/140‑1 (MQ=255)

                                         gATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTgt                                                                                     >  2:1294887/1‑139 (MQ=255)

                                         gATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTgg                                                                                     >  1:1520432/1‑140 (MQ=255)

                                         gATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTgg                                                                                     <  2:466628/140‑1 (MQ=255)

                                         gATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTgg                                                                                     >  1:1360038/1‑140 (MQ=255)

                                          aTCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTggc                                                                                    >  2:81396/1‑140 (MQ=255)

                                          aTCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTggc                                                                                    >  2:586412/1‑140 (MQ=255)

                                          aTCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTggc                                                                                    >  2:1526380/1‑140 (MQ=255)

                                          aTCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTggc                                                                                    <  2:1249012/140‑1 (MQ=255)

                                          aTCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATAATCGGTGTTggc                                                                                    >  1:221904/1‑140 (MQ=255)

                                              ttCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCa                                                                                <  2:808926/140‑1 (MQ=255)

                                              ttCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCa                                                                                <  2:1360038/140‑1 (MQ=255)

                                               tctcTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCAt                                                                               >  1:597899/1‑140 (MQ=255)

                                               tctcTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCAt                                                                               >  2:1436900/1‑140 (MQ=255)

                                                ctctTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATc                                                                              <  1:1146461/140‑1 (MQ=255)

                                                 tctTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCg                                                                             >  1:282764/1‑140 (MQ=255)

                                                   ttGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           <  1:918275/140‑1 (MQ=255)

                                                   ttGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           <  2:1476267/140‑1 (MQ=255)

                                                   ttGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           <  1:2043592/140‑1 (MQ=255)

                                                   ttGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           >  2:518063/1‑140 (MQ=255)

                                                   ttGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           >  2:1986966/1‑140 (MQ=255)

                                                     gTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTc                                                                         >  2:1938046/1‑140 (MQ=255)

                                                        cAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           >  2:1808035/1‑135 (MQ=255)

                                                        cAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGAc                                                                           <  1:1808035/135‑1 (MQ=255)

                                                           ggAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTAt                                                                   >  2:712490/1‑140 (MQ=255)

                                                               tGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCt                                                               >  1:128181/1‑140 (MQ=255)

                                                                gAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTg                                                              >  1:803956/1‑140 (MQ=255)

                                                                gAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTg                                                              >  1:18446/1‑140 (MQ=255)

                                                                     aTTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGt                                                         >  2:478696/1‑140 (MQ=255)

                                                                               aaCCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATtg                                               <  1:1510625/140‑1 (MQ=255)

                                                                               aaCCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATtg                                               <  2:1680708/140‑1 (MQ=255)

                                                                                   ggTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATtgctgc                                           <  2:128181/140‑1 (MQ=255)

                                                                                    gTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCg                                          <  2:18446/140‑1 (MQ=255)

                                                                                    gTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCg                                          <  1:327503/140‑1 (MQ=255)

                                                                                    gTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCg                                          <  2:104690/140‑1 (MQ=255)

                                                                                     tGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCgg                                         >  2:1959288/1‑140 (MQ=255)

                                                                                      ggCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCggg                                        <  2:1520432/140‑1 (MQ=255)

                                                                                      ggCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCggg                                        <  1:478696/140‑1 (MQ=255)

                                                                                      ggCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCggg                                        <  1:586412/140‑1 (MQ=255)

                                                                                      ggCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCggg                                        <  1:712490/140‑1 (MQ=255)

                                                                                          tAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGcc                                    >  1:1879901/1‑140 (MQ=255)

                                                                                           aaGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCt                                   <  2:934701/140‑1 (MQ=255)

                                                                                              tGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCAc                                <  2:546731/140‑1 (MQ=255)

                                                                                                 tcgtcCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCa                                 <  1:955520/136‑1 (MQ=255)

                                                                                                 tcgtcCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTgg                             >  2:708453/1‑140 (MQ=255)

                                                                                                 tcgtcCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTgg                             >  2:2041410/1‑140 (MQ=255)

                                                                                                 tcgtcCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTgg                             >  1:1580311/1‑140 (MQ=255)

                                                                                                 tcgtcCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTgg                             >  1:424108/1‑140 (MQ=255)

                                                                                                      cGCTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCat                        >  2:116032/1‑139 (MQ=255)

                                                                                                        cTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAAt                      >  2:692088/1‑140 (MQ=255)

                                                                                                        cTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAAt                      >  1:341590/1‑140 (MQ=255)

                                                                                                        cTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAAt                      <  1:1436900/140‑1 (MQ=255)

                                                                                                        cTTCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAAt                      >  1:842123/1‑140 (MQ=255)

                                                                                                          tCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAAttt                    >  2:1416665/1‑140 (MQ=255)

                                                                                                          tCAGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAAttt                    >  2:1997525/1‑140 (MQ=255)

                                                                                                            aGTTAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTAt                  <  1:1870341/140‑1 (MQ=255)

                                                                                                              ttAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTAttt                >  2:919495/1‑139 (MQ=255)

                                                                                                              ttAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTAt                  >  1:1466143/1‑138 (MQ=255)

                                                                                                              ttAAAAAGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTAt                  <  2:1466143/138‑1 (MQ=255)

                                                                                                                   aaGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGtt           <  2:1691547/140‑1 (MQ=255)

                                                                                                                   aaGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGtt           >  2:1722151/1‑140 (MQ=255)

                                                                                                                   aaGCACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGGGTTTGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGAGCAAATTTATTCTGGtt           >  1:415067/1‑140 (MQ=255)

                                                                                                                      cACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGttttt        >  2:1848228/1‑139 (MQ=255)

                                                                                                                      cACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTa        >  1:851572/1‑140 (MQ=255)

                                                                                                                      cACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTa        <  1:919495/140‑1 (MQ=255)

                                                                                                                      cACCGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTa        >  2:2121734/1‑140 (MQ=255)

                                                                                                                        ccGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTAtt      >  1:363843/1‑140 (MQ=255)

                                                                                                                        ccGAAATTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTAtt      >  2:1026144/1‑140 (MQ=255)

                                                                                                                            aaTTATTCGCCTCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTa  <  2:39964/140‑1 (MQ=255)

GCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGCGATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGCCGCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTA  >  NC_000913/1005474‑1005737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A