Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	403,595	C→A	A105A (GCC→GCA)	psiF →	PsiF family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	403,595	0	C	A	100.0%	100.9 / NA	29	A105A (GCC→GCA)	psiF	PsiF family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/13); total (16/13)

ACAGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAA  >  NC_000913/403459‑403722

aCAGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAATCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAgcagcc                                                                                                                              <  2:990902/140‑1 (MQ=255)

aCAGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAgcagcc                                                                                                                              <  2:82995/140‑1 (MQ=255)

aCAGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAgcagcc                                                                                                                              <  1:64314/140‑1 (MQ=255)

  aGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGcctg                                                                                                                            >  1:788340/1‑140 (MQ=255)

      aGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGcctgatac                                                                                                                        <  1:1099855/140‑1 (MQ=255)

            cgcCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGcctgatacctgata                                                                                                                  <  2:681703/140‑1 (MQ=255)

            cgcCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGcctgatacctgata                                                                                                                  >  1:681703/1‑140 (MQ=255)

                 ggCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATTAGTGCCTGCCTCAAGAAAGCAGcctgatacctgata                                                                                                                  >  2:219183/1‑135 (MQ=255)

                 ggCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGcctgatacctgata                                                                                                                  <  1:219183/135‑1 (MQ=255)

                 ggCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCt                                                                                                             >  2:211487/1‑140 (MQ=255)

                                        gcagcaAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGc                                                                                      <  1:617052/140‑1 (MQ=255)

                                             aaaaGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCAcc                                                                                 <  2:555526/140‑1 (MQ=255)

                                               aaGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCg                                                                               <  1:211487/140‑1 (MQ=255)

                                               aaGATGCGCAAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCg                                                                               <  2:182627/140‑1 (MQ=255)

                                                         aaTGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATa                                                                     <  1:835036/140‑1 (MQ=255)

                                                                      aGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAg                                                        >  1:489395/1‑140 (MQ=255)

                                                                          gcacaacaATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTcc                                                    <  1:273159/139‑1 (MQ=255)

                                                                          acacaacaATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTcc                                                    <  1:1136961/140‑1 (MQ=255)

                                                                           cacaacaATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCg                                                   >  2:258774/1‑140 (MQ=255)

                                                                            acaacaATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGt                                                  <  2:788340/140‑1 (MQ=255)

                                                                                      ctGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAAtt                                        >  1:105095/1‑140 (MQ=255)

                                                                                        gAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTg                                      >  2:59038/1‑140 (MQ=255)

                                                                                             gTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGaaa                                 >  2:646800/1‑140 (MQ=255)

                                                                                                 tgatCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTg                             >  2:1257731/1‑140 (MQ=255)

                                                                                                    tCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGttt                          <  2:1236574/140‑1 (MQ=255)

                                                                                                           aaGTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATg                   <  1:258774/140‑1 (MQ=255)

                                                                                                             gTTTATGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGtt                 >  1:1193221/1‑140 (MQ=255)

                                                                                                                  tGAGTGCCTGCCTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCaa            >  1:225115/1‑140 (MQ=255)

                                                                                                                      tgcctgccTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAaat        >  2:812485/1‑140 (MQ=255)

                                                                                                                      tgcctgccTCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAaat        >  1:194863/1‑140 (MQ=255)

                                                                                                                       gcctgcctCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAaata       >  1:731772/1‑140 (MQ=255)

                                                                                                                        cctgcctCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAaataa      >  2:1017695/1‑140 (MQ=255)

                                                                                                                            cctCAAGAAAGCAGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATaatgaa  >  1:362399/1‑140 (MQ=255)

ACAGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGCAATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCCTGCCTCAAGAAAGCCGCCTGATACCTGATAGTGCTAACGGGTGAGCTACGAAAATGGCTCACCCGAAATATCATACTTCTGCCTTTAGCTCCGTCTCTATAATTTGGGAAAATTGTTTCTGAATGTTCCCAAAAATAATGAA  >  NC_000913/403459‑403722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A