Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F48 I1 R1 76 74.1 2532288 99.0% 2506965 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 442,633 C→A A362E (GCG→GAG)  thiI → tRNA uridine 4‑sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913442,6330CA96.9% 223.5 / 0.2 65A362E (GCG→GAG) thiItRNA uridine 4‑sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (36/27);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ATCTCTTACGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCAATGGCTTCGG  >  NC_000913/442497‑442762
                                                                                                                                        |                                                                                                                                 
aTCTCTTACGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGga                                                                                                                                >  2:51131/1‑140 (MQ=255)
       aCGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAagaagag                                                                                                                         >  1:5271/1‑140 (MQ=255)
          aCAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAagaagagaat                                                                                                                      >  2:363465/1‑139 (MQ=255)
          aCAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAagaagagaag                                                                                                                      >  1:1164763/1‑140 (MQ=255)
            aaaGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGtt                                                                                                                    <  1:744669/140‑1 (MQ=255)
             aaGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTc                                                                                                                   >  1:1085626/1‑140 (MQ=255)
             aaGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTc                                                                                                                   >  1:1264972/1‑140 (MQ=255)
                     atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAg                                                                                                           >  1:431995/1‑140 (MQ=255)
                     atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAg                                                                                                           >  1:204876/1‑140 (MQ=255)
                      tcatcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGc                                                                                                          >  1:1148779/1‑140 (MQ=255)
                        atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCAt                                                                                                        <  2:318033/140‑1 (MQ=255)
                             ccTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCg                                                                                                   >  2:1109403/1‑140 (MQ=255)
                              cTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGa                                                                                                  <  2:924296/140‑1 (MQ=255)
                              cTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGa                                                                                                  <  2:660842/140‑1 (MQ=255)
                                ggCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATa                                                                                                <  1:1067352/140‑1 (MQ=255)
                                 gCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATaa                                                                                               >  1:379370/1‑140 (MQ=255)
                                  cccGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATaaa                                                                                              >  2:766127/1‑140 (MQ=255)
                                    cGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGt                                                                                            >  2:463088/1‑140 (MQ=255)
                                      ccAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTgg                                                                                          >  2:273117/1‑140 (MQ=255)
                                       cAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGt                                                                                         >  2:138249/1‑140 (MQ=255)
                                            tGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAgg                                                                                    <  1:51131/140‑1 (MQ=255)
                                              gCACCGAAGACTTTGCTCGCACGCTGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGaa                                                                                  <  2:1069457/140‑1 (MQ=255)
                                              gCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGaa                                                                                  <  2:5271/140‑1 (MQ=255)
                                              gCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGaa                                                                                  <  2:379370/140‑1 (MQ=255)
                                                 ccGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCg                                                                               >  1:990082/1‑140 (MQ=255)
                                                   gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGaa                                                                             <  2:762457/140‑1 (MQ=255)
                                                   gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGaa                                                                             <  2:204876/140‑1 (MQ=255)
                                                   gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGaa                                                                             <  2:1085626/140‑1 (MQ=255)
                                                    aaGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGaa                                                                                  <  2:1263318/134‑1 (MQ=255)
                                                    aaGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGaa                                                                                  >  1:1263318/1‑134 (MQ=255)
                                                    aaGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAAt                                                                            >  1:669996/1‑140 (MQ=255)
                                                     aGACTTTGCTCGCACGATGCCGGTATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATa                                                                           <  1:762145/140‑1 (MQ=255)
                                                        cTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACg                                                                        >  2:203998/1‑140 (MQ=255)
                                                                  aCGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCcg                                                              <  1:766127/140‑1 (MQ=255)
                                                                     aTGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGa                                                           <  2:1164763/140‑1 (MQ=255)
                                                                       gCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGaaa                                                         <  1:363465/140‑1 (MQ=255)
                                                                              tatTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACATTGATATCCGCGAAATCGCCca                                                  >  1:1059570/1‑140 (MQ=255)
                                                                                ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCcagc                                                >  1:742851/1‑140 (MQ=255)
                                                                                ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCACGAAATCGCCcagc                                                >  2:425862/1‑140 (MQ=255)
                                                                                   tggtgTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGa                                             >  2:721529/1‑140 (MQ=255)
                                                                                    ggtgTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGAc                                            <  2:742851/140‑1 (MQ=255)
                                                                                    ggtgTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGAc                                            <  1:138249/140‑1 (MQ=255)
                                                                                      tgtgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCg                                          >  1:1093382/1‑140 (MQ=255)
                                                                                       gtgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGa                                         <  1:425862/140‑1 (MQ=255)
                                                                                        tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAg                                        <  1:570707/140‑1 (MQ=255)
                                                                                          aTCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCa                                      >  2:1028414/1‑140 (MQ=255)
                                                                                               cAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAg                                 >  1:1201684/1‑140 (MQ=255)
                                                                                                  aaGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAgtgg                              <  1:1109403/140‑1 (MQ=255)
                                                                                                    gCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAgtggtg                            >  2:457494/1‑140 (MQ=255)
                                                                                                       cGACGGTGAAAGCAGTTAAATCGAAGTTTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGgtggaa                         >  2:892044/1‑140 (MQ=255)
                                                                                                       cGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGgtggaa                         >  1:356425/1‑140 (MQ=255)
                                                                                                       cGACGGTGAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGgtggaa                         >  1:1188541/1‑140 (MQ=255)
                                                                                                           ggTGAAAGGAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGTCCAGCAGACCGAGAAGGAAGTGgtggaagtgg                     <  2:1158422/140‑1 (MQ=255)
                                                                                                              gAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGgtggaagtggaag                  <  1:203998/140‑2 (MQ=255)
                                                                                                              gAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGaaa                  <  1:463088/140‑1 (MQ=255)
                                                                                                              gAAAGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGaaa                  <  2:669996/140‑1 (MQ=255)
                                                                                                               aaaGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCACTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTAATATCCGTTAAATCGCCCAGCATACCGAGCAGGAAGGGGTGGAGGTGGAAAc                 <  2:306304/140‑1 (MQ=255)
                                                                                                                aaGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAAcc                <  1:1045959/140‑1 (MQ=255)
                                                                                                                 aGCAGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCg               <  1:1179637/140‑1 (MQ=255)
                                                                                                                    aGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCa            >  1:921091/1‑140 (MQ=255)
                                                                                                                    aGTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCa            >  1:202726/1‑140 (MQ=255)
                                                                                                                     gTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCaa           <  1:721529/140‑1 (MQ=255)
                                                                                                                     gTTAAATCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCaa           >  1:30434/1‑140 (MQ=255)
                                                                                                                           tCGAAGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCAATGGCtt     >  1:966056/1‑140 (MQ=255)
                                                                                                                              aaGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCAATGGCTTCgg  >  2:959257/1‑140 (MQ=255)
                                                                                                                              aaGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCAATGGCTTCgg  >  1:507872/1‑140 (MQ=255)
                                                                                                                              aaGATTGAAGAGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCAATGGCTTCgg  >  1:1058574/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                 
ATCTCTTACGACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAGCATTCTCGATAAAGTGGTTGAGGAAGCGAATAACGTTGATATCCGCGAAATCGCCCAGCAGACCGAGCAGGAAGTGGTGGAAGTGGAAACCGTCAATGGCTTCGG  >  NC_000913/442497‑442762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A