Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	658,955	G→T	E3D (GAG→GAT)	tatE →	twin arginine protein translocation system ‑ TatE protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	658,955	0	G	T	100.0%	180.8 / NA	51	E3D (GAG→GAT)	tatE	twin arginine protein translocation system ‑ TatE protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (31/20); total (31/20)

TTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCT  >  NC_000913/658823‑659090

tttttACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTa                                                                                                                                  <  2:776343/140‑1 (MQ=255)

      cTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGTCCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCa                                                                                                                            <  2:197499/140‑1 (MQ=255)

               tGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTgg                                                                                                                   <  1:126130/140‑1 (MQ=255)

                          gTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTgcggcg                                                                                                        >  1:764676/1‑140 (MQ=255)

                                   tGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTgcggc                                                                                                         >  1:426053/1‑130 (MQ=255)

                                   tGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTgcggc                                                                                                         <  2:426053/130‑1 (MQ=255)

                                   tGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGtt                                                                                               >  2:228061/1‑140 (MQ=255)

                                   tGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGtt                                                                                               <  1:228061/140‑1 (MQ=255)

                                   tGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGtt                                                                                               >  2:612203/1‑140 (MQ=255)

                                     cTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTct                                                                                             >  2:1197953/1‑140 (MQ=255)

                                      tATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTctg                                                                                            <  1:1178556/140‑1 (MQ=255)

                                             tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTg                                                                                     >  2:959858/1‑140 (MQ=255)

                                             tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTg                                                                                     >  2:141300/1‑140 (MQ=255)

                                             tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTg                                                                                     <  2:438311/140‑1 (MQ=255)

                                               cgcgcgTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTggg                                                                                   >  2:63003/1‑140 (MQ=255)

                                               cgcgcgTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTACGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTggg                                                                                   >  1:228204/1‑140 (MQ=255)

                                                  gcgTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACt                                                                                >  2:988632/1‑140 (MQ=255)

                                                     tCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaaa                                                                             >  2:25526/1‑139 (MQ=255)

                                                            aCCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaagaa                                                                           >  1:1023124/1‑135 (MQ=255)

                                                            aCCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaagaa                                                                           <  2:1023124/135‑1 (MQ=255)

                                                                   atatTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCt                                                               <  2:764676/140‑1 (MQ=255)

                                                                         tctGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCgg                                                         >  1:521615/1‑140 (MQ=255)

                                                                         tctGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCgg                                                         >  2:686001/1‑140 (MQ=255)

                                                                          ctGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGga                                                        <  1:686001/140‑1 (MQ=255)

                                                                           tGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGgat                                                       >  2:23529/1‑139 (MQ=255)

                                                                           tGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGgag                                                       >  2:297308/1‑140 (MQ=255)

                                                                           tGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGgag                                                       >  2:232297/1‑140 (MQ=255)

                                                                              tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGAcc                                                    >  1:31172/1‑140 (MQ=255)

                                                                                      gcgcGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCg                                            <  1:297308/140‑1 (MQ=255)

                                                                                       cgcgTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCgg                                           <  1:1197953/140‑1 (MQ=255)

                                                                                       cgcgTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCgg                                           <  1:141300/140‑1 (MQ=255)

                                                                                             cTGTTGCCGGTTATATGTCAAGAAGGTAACTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTa                                     <  1:1154062/140‑1 (MQ=255)

                                                                                              tGTTGCCGGTTATATGTCAAGAAGGTATCTACGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTaa                                    <  2:228204/140‑1 (MQ=255)

                                                                                                   ccGGTTATATGTCAAGAAGGAATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGt                               >  1:131604/1‑140 (MQ=255)

                                                                                                      gTTATATGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCa                            >  1:1164010/1‑140 (MQ=255)

                                                                                                        tataTGTCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCaag                          <  1:988632/140‑1 (MQ=255)

                                                                                                              tCAAGAAGGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCg                    <  2:521615/140‑1 (MQ=255)

                                                                                                                aagaagGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGat                  >  2:720930/1‑140 (MQ=255)

                                                                                                                aagaagGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGat                  >  1:1255160/1‑140 (MQ=255)

                                                                                                                 agaagGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGatt                 >  2:994251/1‑139 (MQ=255)

                                                                                                                 agaagGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGatg                 >  1:361293/1‑140 (MQ=255)

                                                                                                                    agGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGatgaat              <  2:1164010/140‑1 (MQ=255)

                                                                                                                    agGTATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGatgaat              <  1:23529/140‑1 (MQ=255)

                                                                                                                       tATCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAatgat           >  2:1051490/1‑140 (MQ=255)

                                                                                                                        aTCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAatgatg          >  1:705887/1‑140 (MQ=255)

                                                                                                                        aTCTATGGGTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAatgatg          >  1:1100711/1‑140 (MQ=255)

                                                                                                                               ggTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGat                  >  2:1231487/1‑125 (MQ=255)

                                                                                                                               ggTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGat                  <  1:1231487/125‑1 (MQ=255)

                                                                                                                                gTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGAtgct  >  1:136861/1‑140 (MQ=255)

                                                                                                                                gTGATATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGAtgct  >  1:136700/1‑140 (MQ=255)

                                                                                                                                gTGATATTAGTATTACCAAACTGCTGGGAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTGACGTACGCTGGGCGGATACCTTGGAGCGGCCATTAAAGGGGTCAAGAAGGCGATGAATGATGACGAtgct  >  1:287216/1‑140 (MQ=255)

TTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCT  >  NC_000913/658823‑659090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A