Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	663,900	C→A	A22S (GCC→TCC)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase DacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	663,900	0	C	A	100.0%	192.1 / NA	57	A22S (GCC→TCC)	dacA	D‑alanyl‑D‑alanine carboxypeptidase DacA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (25/32); total (25/32)

TCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCAT  >  NC_000913/663767‑664035

tCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCa                                                                                                                                   <  2:1169322/140‑1 (MQ=255)

tCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCa                                                                                                                                   <  1:813996/140‑1 (MQ=255)

acTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCGTAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCa                                                                                                                                   <  1:25842/139‑1 (MQ=255)

  tGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCaaa                                                                                                                                 <  1:126770/140‑1 (MQ=255)

           ttCGGCGCGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCgtgg                                                                                                                        >  1:1226623/1‑140 (MQ=255)

           ttCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCgtgg                                                                                                                        >  2:265965/1‑140 (MQ=255)

                  aGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAgcgc                                                                                                                 >  1:186605/1‑140 (MQ=255)

                     aCTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAg                                                                                                              <  2:821460/140‑1 (MQ=255)

                       tttGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGgc                                                                                                            <  2:1019487/140‑1 (MQ=255)

                        ttGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGgcg                                                                                                           <  2:1226623/140‑1 (MQ=255)

                         tGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGgcgc                                                                                                          >  1:1028985/1‑140 (MQ=255)

                         tGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATATGCGGTACACGCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATATGCAAAGAGCCGTGGTGAGCGCCAGgcgc                                                                                                          <  1:599850/140‑1 (MQ=255)

                               aGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATg                                                                                                    >  1:873808/1‑140 (MQ=255)

                                   ataGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGGGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATAc                                                                                                >  1:154086/1‑140 (MQ=255)

                                         aaTCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCgg                                                                                          >  1:1156539/1‑140 (MQ=255)

                                         aaTCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCgg                                                                                          >  1:505479/1‑140 (MQ=255)

                                         aaTCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCgg                                                                                          >  1:426692/1‑140 (MQ=255)

                                          aTCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGATTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGa                                                                                         >  2:1006151/1‑140 (MQ=255)

                                           tCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaa                                                                                        >  2:1241858/1‑140 (MQ=255)

                                           tCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaa                                                                                        >  1:881500/1‑140 (MQ=255)

                                           tCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaa                                                                                        >  1:885602/1‑140 (MQ=255)

                                           tCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATATGTAACAGAGAATAAAGATTTTCAAAGATCCGTGGTTAGCGCCAGGCGCTTTATGATACTAGCGGaa                                                                                        >  2:558445/1‑140 (MQ=255)

                                                 tGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATATGTAAATAAACGTGGTGGGCGCCAGGCGCTTCATGATACGAGCGGTAAAAAtt                                                                                  >  2:603133/1‑139 (MQ=255)

                                                       aCTCCGCATTGAACTGCGGTACAGCCGGGATCATAGTTTTGATATTAAGGTCATCGGCATGTTAAGCAGAGATAAAGGATGTGCAAAGAGCCGTCGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTc                                                                            <  2:1081957/140‑1 (MQ=255)

                                                       aCTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTc                                                                            <  1:1146072/140‑1 (MQ=255)

                                                       aCTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTc                                                                            <  1:623707/140‑1 (MQ=255)

                                                       aCTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTc                                                                            <  2:1028985/140‑1 (MQ=255)

                                                         tCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCAt                                                                          >  1:380658/1‑140 (MQ=255)

                                                         tCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAAGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCAt                                                                          >  2:42789/1‑140 (MQ=255)

                                                           cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATgg                                                                        >  2:126192/1‑140 (MQ=255)

                                                           cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATgg                                                                        >  1:30472/1‑140 (MQ=255)

                                                           cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATgg                                                                        >  1:969652/1‑140 (MQ=255)

                                                           cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATgg                                                                        >  2:759821/1‑140 (MQ=255)

                                                           cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAACCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGGATTCATgg                                                                        >  2:1200221/1‑140 (MQ=255)

                                                           cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGAGAAAGGATTTGCTAAGAGCCGTGGTGGGCGCCAGGCGCTTCATGATACGAGCGGGACAAATGGTATTCATgg                                                                        >  2:701450/1‑140 (MQ=255)

                                                            gCATCGATCTGCGGTACACCGGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGt                                                                       <  2:154086/140‑1 (MQ=255)

                                                            gCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGt                                                                       <  2:885602/140‑1 (MQ=255)

                                                            gCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGt                                                                       <  2:436401/140‑1 (MQ=255)

                                                             cATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTc                                                                      <  1:265965/140‑1 (MQ=255)

                                                                cGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGa                                                                   <  2:426692/140‑1 (MQ=255)

                                                                  aacTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGAGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGAGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAAc                                                                 <  1:558445/138‑1 (MQ=255)

                                                                   tCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACt                                                                >  2:32905/1‑140 (MQ=255)

                                                                            cacCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCGGCAGAGAAAAAGGGTTTGCAAAAAACTGCGGTGAACCCCAAGCGCTTCATGTTACGATAGGTAAAAATGGTATTCATGGTCTGAACTACGACATcc                                                       >  2:1100245/1‑140 (MQ=255)

                                                                                 gggATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGAt                                                  >  1:825741/1‑140 (MQ=255)

                                                                                 gggATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGAt                                                  >  2:1103948/1‑140 (MQ=255)

                                                                                   gATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATgg                                                <  1:1100245/140‑1 (MQ=255)

                                                                                   gATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATgg                                                <  1:1241858/140‑1 (MQ=255)

                                                                                             ttGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTaaaaaa                                      >  2:241825/1‑140 (MQ=255)

                                                                                             ttGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTaaaaaa                                      <  2:825741/140‑1 (MQ=255)

                                                                                                atatTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGATCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTg                                   <  2:1156539/140‑1 (MQ=255)

                                                                                                 tatTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGc                                  <  2:1216665/140‑1 (MQ=255)

                                                                                                 aatTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGc                                  <  2:969652/139‑1 (MQ=255)

                                                                                                 aatTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGc                                  <  2:380658/139‑1 (MQ=255)

                                                                                                  atTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGcc                                 <  1:759821/140‑1 (MQ=255)

                                                                                                  atTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGcc                                 <  1:416511/140‑1 (MQ=255)

                                                                                                     cAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCctt                              >  2:223132/1‑139 (MQ=255)

                                                                                                     cAGGTCATCGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCcta                              <  1:87361/140‑1 (MQ=255)

                                                                                                             cGGCATGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCa                      <  1:126192/140‑1 (MQ=255)

                                                                                                                 aTGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATg                  <  2:881500/140‑1 (MQ=255)

                                                                                                                 aTGTGCAGCAGAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATg                  <  2:186605/140‑1 (MQ=255)

                                                                                                                  tgtgCAGGAGAGATAAAGGTTTTGCAAAAAGACGCGGTGAGTGCCAGGTGCTTCATGATACGTGCGGAAAAAAGGGGATTCATAGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTTCTTTAGCATATGc                 >  2:661919/1‑140 (MQ=255)

                                                                                                                         cagAGATAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCg          >  1:519676/1‑140 (MQ=255)

                                                                                                                              aTAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCggcagg     >  1:375368/1‑140 (MQ=255)

                                                                                                                               tAAAGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCggcaggc    <  1:32905/140‑1 (MQ=255)

                                                                                                                                 aaGGATGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCAt  <  2:30472/140‑1 (MQ=255)

TCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCAT  >  NC_000913/663767‑664035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A