Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	843,669	C→A	G604G (GGG→GGT)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	843,669	0	C	A	98.3%	200.8 / NA	59	G604G (GGG→GGT)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (34/24); minor base T (0/1); total (34/25)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCTTTCAGTGCCTGATTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTT  >  NC_000913/843534‑843803

cGCATCTTTCAGTGCCTGATTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCgg                                                                                                                                    >  2:676248/1‑140 (MQ=255)

  cATCTTTCAGTGCCTGATTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTa                                                                                                                                  >  2:21007/1‑140 (MQ=255)

         cAGTGCCTGATTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTc                                                                                                                           >  1:701594/1‑140 (MQ=255)

              ccTGATTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACg                                                                                                                      >  2:895924/1‑140 (MQ=255)

                  aTTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCa                                                                                                                  >  2:1209097/1‑140 (MQ=255)

                  aTTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCa                                                                                                                  >  2:1028232/1‑140 (MQ=255)

                  aTTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCa                                                                                                                  >  2:722806/1‑140 (MQ=255)

                    tGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACg                                                                                                                <  1:722806/140‑1 (MQ=255)

                     ggCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACgg                                                                                                               <  1:821662/140‑1 (MQ=255)

                      gCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGCTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGa                                                                                                              >  2:634397/1‑140 (MQ=255)

                       cTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAg                                                                                                             <  1:861918/140‑1 (MQ=255)

                              aGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCg                                                                                                      <  2:665984/140‑1 (MQ=255)

                                cATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGAc                                                                                                    >  2:1107585/1‑140 (MQ=255)

                                cATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGAc                                                                                                    >  2:157787/1‑140 (MQ=255)

                                cATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGAc                                                                                                    <  1:1209097/140‑1 (MQ=255)

                                cATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGAc                                                                                                    >  2:1153119/1‑140 (MQ=255)

                                aaTCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCTACGGTATCCTGTCGTACGCCCACGGAGCGAATCGAc                                                                                                    <  2:363078/139‑1 (MQ=255)

                                     tCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCg                                                                                               <  1:21007/140‑1 (MQ=255)

                                      cATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGt                                                                                              <  1:634397/140‑1 (MQ=255)

                                        cggCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTc                                                                                            >  1:1253739/2‑140 (MQ=255)

                                         ggCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTcc                                                                                           >  2:127737/1‑140 (MQ=255)

                                          gCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCa                                                                                          >  2:1205829/1‑140 (MQ=255)

                                          gCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCa                                                                                          <  1:833602/140‑1 (MQ=255)

                                              tCAACATCATAATTTGTCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACtgt                                                                                      >  1:896058/1‑140 (MQ=255)

                                              tCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACtgt                                                                                      >  1:780702/1‑140 (MQ=255)

                                                   atcatAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCgg                                                                                 >  2:627138/1‑140 (MQ=255)

                                                     catAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTc                                                                               <  1:895924/140‑1 (MQ=255)

                                                       tAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCaa                                                                             <  1:676248/140‑1 (MQ=255)

                                                       tAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCaa                                                                             <  2:1253739/140‑1 (MQ=255)

                                                             gCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGccc                                                                       >  2:15015/1‑140 (MQ=255)

                                                               aaccaccGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTc                                                                               <  2:723788/129‑1 (MQ=255)

                                                               aaccaccGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTc                                                                               >  1:723788/2‑130 (MQ=255)

                                                                   accGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGt                                                                 <  1:1205829/140‑1 (MQ=255)

                                                                     cGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCa                                                               >  2:619361/1‑140 (MQ=255)

                                                                      gAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCAc                                                              <  1:1107585/140‑1 (MQ=255)

                                                                      gAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCAc                                                              <  2:701594/140‑1 (MQ=255)

                                                                       aaCCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCAcc                                                             >  2:761890/1‑140 (MQ=255)

                                                                        aCCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCa                                                            >  2:1197133/1‑140 (MQ=255)

                                                                           aaTGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAAt                                                         <  2:896058/140‑1 (MQ=255)

                                                                            aTGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAAtc                                                        >  1:38456/1‑140 (MQ=255)

                                                                             tGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAAtct                                                       >  1:616218/1‑140 (MQ=255)

                                                                                   ggACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAgtg                                                 >  1:456272/1‑140 (MQ=255)

                                                                                          gTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGc                                          <  1:127737/140‑1 (MQ=255)

                                                                                               cAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGttt                                     >  2:70706/1‑140 (MQ=255)

                                                                                                 aaGGTGGTTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTc                                   >  1:1160313/1‑140 (MQ=255)

                                                                                                   ggtggtTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCaa                                 <  1:761890/140‑1 (MQ=255)

                                                                                                   ggtggtTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCaa                                 >  2:94724/1‑140 (MQ=255)

                                                                                                   ggtggtTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCaa                                 <  2:563134/140‑1 (MQ=255)

                                                                                                     tggtTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAAc                               >  2:1143165/1‑140 (MQ=255)

                                                                                                     tggtTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAAc                               >  1:851563/1‑140 (MQ=255)

                                                                                                       gtTATCGAAGACCACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTg                             <  1:627138/140‑1 (MQ=255)

                                                                                                                  ccACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATTccc                  <  2:780702/140‑1 (MQ=255)

                                                                                                                   cACGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAcccc                 <  2:38456/140‑1 (MQ=255)

                                                                                                                     cGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCgt               >  2:369421/1‑139 (MQ=255)

                                                                                                                     cGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCgg               >  1:642504/1‑140 (MQ=255)

                                                                                                                     cGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCg                <  2:338923/139‑1 (MQ=255)

                                                                                                                     cGGAATGATGTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCg                >  1:338923/1‑139 (MQ=255)

                                                                                                                           gatgTGCTACACACCGGTATCATGTCGTACCCCCACGGATTGAATCGCGATCAGTTCCACTGTGCACCGCACCGGCCCGATAGTCACCAAATCCCCAGTGTCCATGCCGTTTTCAGGCTAAATCAATACCCCGGTGataa         <  2:1233663/140‑1 (MQ=255)

                                                                                                                           gatgTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGataa         <  1:619361/140‑1 (MQ=255)

                                                                                                                           aatgTGATACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGACACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGataa         <  2:99844/139‑1 (MQ=255)

                                                                                                                                  tACGCACCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCttt  >  1:7404/1‑140 (MQ=255)

CGCATCTTTCAGTGCCTGATTGGCTTTATCAGCATCTTCATGGCGATCAACATCATAATTTGCCACCACCGAACCAATGCCGCGGACAAAGTTAGCAAAGGTGGTTATCGAAGACCACGGAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTT  >  NC_000913/843534‑843803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A