Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F48 I1 R1 61 131.2 4447032 99.1% 4407008 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 368,178 T→C E81E (GAA→GAG)  mhpR ← DNA‑binding transcriptional activator MhpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913368,1780TC99.2% 521.4 / ‑4.7 133E81E (GAA→GAG) mhpRDNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (76/56);  total (77/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 134 total aligned reads displayed.

GAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGC  >  NC_000913/368048‑368313
                                                                                                                                  |                                                                                                                                       
gAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAAt                                                                                                                                >  1:1384549/1‑140 (MQ=255)
     gTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCg                                                                                                                           >  1:375020/1‑140 (MQ=255)
     gTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCg                                                                                                                           <  1:78822/140‑1 (MQ=255)
     gTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCg                                                                                                                           <  1:1063505/140‑1 (MQ=255)
      tttCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGc                                                                                                                          <  2:1186857/140‑1 (MQ=255)
       ttCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCa                                                                                                                         <  2:1919458/140‑1 (MQ=255)
       ttCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCa                                                                                                                         <  2:1895312/140‑1 (MQ=255)
       ttCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCa                                                                                                                         <  1:894846/140‑1 (MQ=255)
        tCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCAc                                                                                                                        >  1:2097110/1‑140 (MQ=255)
           cgTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACttt                                                                                                                     >  2:526111/1‑140 (MQ=255)
           cgTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACttt                                                                                                                     >  1:1671356/1‑140 (MQ=255)
             tACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCATTGTTAGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGa                                                                                                                   >  1:726360/1‑140 (MQ=255)
              accaccATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGAt                                                                                                                  <  2:245711/140‑1 (MQ=255)
                caccaTTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATgg                                                                                                                >  1:1307901/1‑140 (MQ=255)
                 accaTTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGt                                                                                                               <  1:2205794/140‑1 (MQ=255)
                        cATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGa                                                                                                        >  2:2223089/1‑140 (MQ=255)
                        cATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGa                                                                                                        >  1:377739/1‑140 (MQ=255)
                          tCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaaa                                                                                                      >  1:1129203/1‑140 (MQ=255)
                          tCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaaa                                                                                                      >  1:369462/1‑140 (MQ=255)
                          tCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCAGCACTTTGATGGTCAGTCGaaa                                                                                                      >  1:914884/1‑140 (MQ=255)
                           cAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaaaa                                                                                                     <  2:249393/140‑1 (MQ=255)
                            aaCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaaaa                                                                                                     <  1:647397/139‑1 (MQ=255)
                            aaCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAAc                                                                                                    <  2:1307901/140‑1 (MQ=255)
                            aaCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGCTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaaaat                                                                                                    >  1:808880/1‑139 (MQ=255)
                             aCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACt                                                                                                   >  1:901658/1‑140 (MQ=255)
                             aCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACt                                                                                                   >  1:1023087/1‑140 (MQ=255)
                             aCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACt                                                                                                   >  2:375045/1‑140 (MQ=255)
                                tCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatc                                                                                                >  1:2058625/1‑140 (MQ=255)
                                tCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatc                                                                                                >  2:1949210/1‑140 (MQ=255)
                                tCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatc                                                                                                >  2:2108700/1‑140 (MQ=255)
                                    gCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCg                                                                                            >  1:1336249/1‑140 (MQ=255)
                                     cGTGGACACATCTGTCGGCCATACCTATTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCgg                                                                                           <  1:2004457/140‑1 (MQ=255)
                                     cGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCgg                                                                                           >  1:334440/1‑140 (MQ=255)
                                      gTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGa                                                                                          >  1:1202620/1‑140 (MQ=255)
                                         gACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAggg                                                                                       >  2:1551309/1‑140 (MQ=255)
                                          acacATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAgggg                                                                                      <  2:1805330/140‑1 (MQ=255)
                                           aacaTCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCATTGTTAGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGc                                                                                     <  2:726360/139‑1 (MQ=255)
                                           aacaTCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGc                                                                                     <  2:702254/139‑1 (MQ=255)
                                               tCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcg                                                                                 <  2:375020/140‑1 (MQ=255)
                                               tCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcg                                                                                 <  1:1461100/140‑1 (MQ=255)
                                               tCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcg                                                                                 <  2:726654/140‑1 (MQ=255)
                                               tCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcg                                                                                 >  2:1379710/1‑140 (MQ=255)
                                               tCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcg                                                                                 <  2:384846/140‑1 (MQ=255)
                                                cTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcgg                                                                                >  1:1854017/1‑140 (MQ=255)
                                                cTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcgg                                                                                <  1:1218478/140‑1 (MQ=255)
                                                 tGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcggc                                                                               <  1:1306293/140‑1 (MQ=255)
                                                 tGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcggc                                                                               <  2:1958276/140‑1 (MQ=255)
                                                       ccATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCACCGGAGGGGCTACGGCGGACat                                                                         <  2:402090/140‑1 (MQ=255)
                                                          tACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcggc                                                                               >  1:924813/1‑131 (MQ=255)
                                                          tACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcggc                                                                               <  2:924813/131‑1 (MQ=255)
                                                          tACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATc                                                                      >  1:2022255/1‑140 (MQ=255)
                                                               cTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCctct                                                                 <  1:526111/140‑1 (MQ=255)
                                                               cTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCctct                                                                 >  1:291934/1‑140 (MQ=255)
                                                                ttCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCtt                                                                >  2:1244257/1‑140 (MQ=255)
                                                                 tCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTc                                                               >  2:431465/1‑140 (MQ=255)
                                                                    cgcAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCtt                                                            >  2:862546/1‑139 (MQ=255)
                                                                    cgcAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTg                                                            >  1:2201826/1‑140 (MQ=255)
                                                                      aaaCAGAGCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCTTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCa                                                          <  2:808880/139‑1 (MQ=255)
                                                                        aCAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGc                                                        <  1:1553914/140‑1 (MQ=255)
                                                                         cAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCg                                                       <  1:303824/140‑1 (MQ=255)
                                                                         cAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCg                                                       >  2:1651475/1‑140 (MQ=255)
                                                                            aTCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGtct                                                    >  1:666814/1‑140 (MQ=255)
                                                                            aTCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGtct                                                    >  1:935020/1‑140 (MQ=255)
                                                                            aTCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGtct                                                    >  2:1668514/1‑140 (MQ=255)
                                                                            aTCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGtct                                                    <  1:1949210/140‑1 (MQ=255)
                                                                               gCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCca                                                 >  2:1511996/1‑140 (MQ=255)
                                                                                 ccAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCcagc                                               >  1:1508878/1‑140 (MQ=255)
                                                                                 ccAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCcagc                                               <  1:2108700/140‑1 (MQ=255)
                                                                                 acagcagTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGTCATATCCCTCTTCCTGCAGCGTCTCcagc                                               <  2:914884/139‑1 (MQ=255)
                                                                                     cagTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTc                                           >  2:385926/1‑140 (MQ=255)
                                                                                     cagTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTc                                           >  2:1969202/1‑140 (MQ=255)
                                                                                      agTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTcg                                          <  2:1880179/140‑1 (MQ=255)
                                                                                      agTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTcg                                          <  2:474689/140‑1 (MQ=255)
                                                                                      agTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTcg                                          <  2:2179036/140‑1 (MQ=255)
                                                                                        tGGGGCCGCCAGTGCAGAAATCCACTGTTCGTAACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTcgcc                                        >  1:545622/1‑140 (MQ=255)
                                                                                         ggggCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCAACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTcgccg                                       >  2:1825635/1‑140 (MQ=255)
                                                                                          gggCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCTTCTCCAGCCGTcgccgc                                      >  2:167103/1‑140 (MQ=255)
                                                                                          gggCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTcgccgc                                      >  2:340040/1‑140 (MQ=255)
                                                                                           ggCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCTCTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTATTCCTGCAGCGTCTCCAGCAGTCGCCGca                                     <  1:1609266/140‑1 (MQ=255)
                                                                                           ggCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGca                                     <  1:1969202/140‑1 (MQ=255)
                                                                                           ggCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGca                                     <  2:1475137/140‑1 (MQ=255)
                                                                                              cgccAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAg                                  <  1:1825635/140‑1 (MQ=255)
                                                                                              cgccAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAg                                  <  1:1511996/140‑1 (MQ=255)
                                                                                               gccAGTGCATAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAgt                                 >  2:627322/1‑140 (MQ=255)
                                                                                               gccAGTGCAGAAATCCACTGTTCGTCAAGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAgt                                 >  1:1804617/1‑140 (MQ=255)
                                                                                                      cAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGa                          >  2:453878/1‑140 (MQ=255)
                                                                                                       aGAAATCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGAt                         <  2:1384549/140‑1 (MQ=255)
                                                                                                            tCCACTGTTCGTCACGAAATCCCTCGCTTAATTGCCACACTTTGATGGTCAGTCGCAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAgg                    >  1:1454068/1‑140 (MQ=255)
                                                                                                              cACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGcc                  >  2:1718928/1‑140 (MQ=255)
                                                                                                              cACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGcc                  >  2:503260/1‑140 (MQ=255)
                                                                                                              cACTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGcc                  >  2:785147/1‑140 (MQ=255)
                                                                                                                cTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGc                >  1:1482621/1‑140 (MQ=255)
                                                                                                                cTGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGc                <  2:2054160/140‑1 (MQ=255)
                                                                                                                 tGTTCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCt               >  1:1184074/1‑140 (MQ=255)
                                                                                                                   ttCGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGa             >  1:2218647/1‑140 (MQ=255)
                                                                                                                     cGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGt           >  1:591753/1‑140 (MQ=255)
                                                                                                                     cGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGt           >  1:2020010/1‑140 (MQ=255)
                                                                                                                     cGTCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGt           <  1:763863/140‑1 (MQ=255)
                                                                                                                       tCACGAAATCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGCGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGCGGTGCGATGCAGGCCGCTGAGGTc         >  1:1942252/1‑140 (MQ=255)
                                                                                                                              aTCCCTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCcagc  <  2:1202620/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
GAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCCAGCAGTCGCCGCACAGTGGTGCGATGCAGGCCGCTGAGTTCCGCCAGC  >  NC_000913/368048‑368313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A