Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F48 I1 R1 61 131.2 4447032 99.1% 4407008 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 425,791 C→A L261M (CTG→ATG)  queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913425,7910CA100.0% 535.4 / NA 152L261M (CTG→ATG) queAtRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (82/70);  total (82/70)

Only 100 of 152 total aligned reads displayed.

CGCGTCGACACCATTGAAGATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGCTGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCAGAGTCGAC  >  NC_000913/425656‑425927
                                                                                                                                       |                                                                                                                                        
cgcgTCGACACCATTGAAGATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGa                                                                                                                                      <  1:718093/140‑1 (MQ=255)
                aaGATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCa                                                                                                                      >  2:1681855/1‑140 (MQ=255)
                  gATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGc                                                                                                                    <  2:2095353/140‑1 (MQ=255)
                  gATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGc                                                                                                                    >  1:544598/1‑140 (MQ=255)
                     cacaTCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaa                                                                                                                 >  2:204553/1‑140 (MQ=255)
                     cacaTCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaa                                                                                                                 >  2:2047245/1‑140 (MQ=255)
                     cacaTCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaa                                                                                                                 >  1:270699/1‑140 (MQ=255)
                     cacaTCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaa                                                                                                                 >  2:360826/1‑140 (MQ=255)
                      acaTCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaaa                                                                                                                >  1:1282250/1‑140 (MQ=255)
                       catcatGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaaaa                                                                                                               >  1:842888/1‑140 (MQ=255)
                        atcatGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGaaa                                                                                                                >  1:527855/1‑138 (MQ=255)
                          catGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACg                                                                                                            >  2:923999/1‑140 (MQ=255)
                          catGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACg                                                                                                            >  1:1855330/1‑140 (MQ=255)
                          catGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACg                                                                                                            >  2:655154/1‑140 (MQ=255)
                           atGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGTGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGTCAGCGAAAAACGa                                                                                                           >  1:671489/1‑140 (MQ=255)
                           atGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGa                                                                                                           <  1:1711071/140‑1 (MQ=255)
                            tGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGAt                                                                                                          >  2:1550931/1‑140 (MQ=255)
                             gCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGAtc                                                                                                         >  1:1145757/1‑140 (MQ=255)
                              cACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGAtct                                                                                                        >  1:1311236/1‑140 (MQ=255)
                               aCTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGAtctc                                                                                                       >  1:731176/1‑140 (MQ=255)
                                cTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCa                                                                                                      <  2:24932/140‑1 (MQ=255)
                                cTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCa                                                                                                      >  2:1618460/1‑140 (MQ=255)
                                 tCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCAt                                                                                                     >  1:1722449/1‑140 (MQ=255)
                                  cGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCAtt                                                                                                    >  1:430368/1‑140 (MQ=255)
                                  cGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCAtt                                                                                                    >  2:585029/1‑140 (MQ=255)
                                    gAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGa                                                                                                  >  1:1268988/1‑140 (MQ=255)
                                     aaTACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGaa                                                                                                 <  1:1550931/140‑1 (MQ=255)
                                              aaGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGttcttc                                                                                        >  2:1497478/1‑140 (MQ=255)
                                              aaGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGttcttc                                                                                        >  2:310368/1‑140 (MQ=255)
                                              aaGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGttcttc                                                                                        >  2:469663/1‑140 (MQ=255)
                                               aGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATTGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTcg                                                                                       >  1:1764712/1‑140 (MQ=255)
                                                  aCCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTcgacg                                                                                    <  2:430368/140‑1 (MQ=255)
                                                   ccGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTcgacga                                                                                   <  2:1417171/140‑1 (MQ=255)
                                                   ccGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTcgacga                                                                                   <  1:923999/140‑1 (MQ=255)
                                                    cGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAAc                                                                                                <  2:184294/126‑1 (MQ=255)
                                                      cAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAc                                                                                >  1:333703/1‑140 (MQ=255)
                                                      cAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAc                                                                                >  1:1318140/1‑140 (MQ=255)
                                                      cAGGATGTGGTAGACGCGGTACTGGCGGCAAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAc                                                                                >  1:1963282/1‑140 (MQ=255)
                                                      cAGGATGTGGTAGACGCGGTACTGGCAGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAc                                                                                >  1:1453910/1‑140 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  2:270699/140‑1 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  1:968700/140‑1 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  2:544598/140‑1 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              >  2:754244/1‑140 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  2:952983/140‑1 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  1:360826/140‑1 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  1:469663/140‑1 (MQ=255)
                                                        ggATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATAccc                                                                              <  1:1497478/140‑1 (MQ=255)
                                                              ggTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCt                                                                        <  1:585029/140‑1 (MQ=255)
                                                                tAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCttt                                                                      <  1:773938/140‑1 (MQ=255)
                                                                  gACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtat                                                                    <  2:1319559/140‑1 (MQ=255)
                                                                   aCGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatc                                                                   >  2:1994839/1‑140 (MQ=255)
                                                                   aCGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatc                                                                   >  2:1303882/1‑140 (MQ=255)
                                                                   aCGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatc                                                                   >  1:1987060/1‑140 (MQ=255)
                                                                   aCGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatc                                                                   >  1:762160/1‑140 (MQ=255)
                                                                     gcgGTACTGGCGGCGAAAGCGCGCGGGAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatcta                                                                 >  2:1555036/1‑140 (MQ=255)
                                                                       ggcaCTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatctatc                                                               >  1:625088/4‑140 (MQ=255)
                                                                       ggTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatctatc                                                               <  2:1988545/140‑1 (MQ=255)
                                                                       ggTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatctatc                                                               >  2:1864923/1‑140 (MQ=255)
                                                                       ggTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatctatc                                                               >  2:715749/1‑140 (MQ=255)
                                                                       ggTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTtatctatc                                                               >  2:2061076/1‑140 (MQ=255)
                                                                          aCTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCgg                                                            <  1:1362484/140‑1 (MQ=255)
                                                                          aCTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCgg                                                            <  2:1257659/140‑1 (MQ=255)
                                                                          aCTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCgg                                                            <  1:1994839/140‑1 (MQ=255)
                                                                          aCTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCgg                                                            <  2:762160/140‑1 (MQ=255)
                                                                          aCTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCgg                                                            <  1:204553/140‑1 (MQ=255)
                                                                            tGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGc                                                          <  1:655154/140‑1 (MQ=255)
                                                                              gcggcgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCtt                                                        <  2:1145757/140‑1 (MQ=255)
                                                                              gcggcgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCtt                                                        >  1:1227941/1‑140 (MQ=255)
                                                                               cggcgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGCTACCCAAACCTTTATCTATCCGGGCTTc                                                       <  1:2089043/140‑1 (MQ=255)
                                                                               cggcgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTc                                                       <  1:267423/140‑1 (MQ=255)
                                                                               cggcgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTc                                                       <  2:249296/140‑1 (MQ=255)
                                                                               cggcgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTc                                                       <  1:1151716/140‑1 (MQ=255)
                                                                                  cgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGc                                                          <  2:1323931/134‑1 (MQ=255)
                                                                                  cgAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGc                                                          >  1:1323931/1‑134 (MQ=255)
                                                                                   gAAAGCGCGCGGTAACCGGGGGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCTGGCTTCCAGt                                                   >  1:605758/1‑140 (MQ=255)
                                                                                      aGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACa                                                <  2:1963282/140‑1 (MQ=255)
                                                                                        cgcgcgGTAACCGGGTGATTGCGGTCGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACaaa                                              >  2:186483/1‑140 (MQ=255)
                                                                                         gcgcgGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAg                                             <  1:1681855/140‑1 (MQ=255)
                                                                                          cgcgGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGt                                            >  1:1846942/1‑140 (MQ=255)
                                                                                             ggTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGt                                         <  2:1106062/140‑1 (MQ=255)
                                                                                                aaCCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCATCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGa                                      <  2:671489/140‑1 (MQ=255)
                                                                                                 aCCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGAt                                     <  2:375763/140‑1 (MQ=255)
                                                                                                 aCCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGAt                                     <  2:1292527/140‑1 (MQ=255)
                                                                                                    gggTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATgcg                                  <  1:205414/140‑1 (MQ=255)
                                                                                                    gggTGATTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATgcg                                  <  1:1555036/140‑1 (MQ=255)
                                                                                                         aTTGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACACAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGt                             >  2:1256904/1‑140 (MQ=255)
                                                                                                           tGCGGTTGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGa                           >  1:999571/1‑140 (MQ=255)
                                                                                                                ttGGCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAAc                      >  1:895861/1‑140 (MQ=255)
                                                                                                                  ggCACCACTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACtt                    <  2:731176/140‑1 (MQ=255)
                                                                                                                    caccacTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTcc                  >  2:1086592/1‑140 (MQ=255)
                                                                                                                     accacTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCa                 <  2:1764712/140‑1 (MQ=255)
                                                                                                                     accacTTCAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCa                 <  2:916252/140‑1 (MQ=255)
                                                                                                                            cAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGCACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCa          >  2:1734064/1‑140 (MQ=255)
                                                                                                                            cAGTACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCa          >  2:1844044/1‑140 (MQ=255)
                                                                                                                               tACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCagag       >  1:636631/1‑140 (MQ=255)
                                                                                                                               tACGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCagag       <  1:1023937/140‑1 (MQ=255)
                                                                                                                                 cGTTCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCAGAGTc     >  1:1224812/1‑140 (MQ=255)
                                                                                                                                    tCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCAGAGTCGAc  >  1:1081749/1‑140 (MQ=255)
                                                                                                                                    tCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCAGAGTCGAc  <  2:1215403/140‑1 (MQ=255)
                                                                                                                                    tCGATGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCAGAGTCGAc  >  1:2106077/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CGCGTCGACACCATTGAAGATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTACTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGCTGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTCCACTTGCCAGAGTCGAC  >  NC_000913/425656‑425927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A