Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	420,869	G→T	D427Y (GAT→TAT)	brnQ →	branched chain amino acid transporter BrnQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	420,869	0	G	T	98.7%	255.2 / NA	76	D427Y (GAT→TAT)	brnQ	branched chain amino acid transporter BrnQ
Reads supporting (aligned to +/- strand): ref base G (0/1); major base T (32/42); minor base C (1/0); total (33/43)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAA  >  NC_000913/420738‑420996

ttCTCGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATcgcgcg                                                                                                                         >  1:450103/1‑140 (MQ=255)

ttCTCGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATcgcgcg                                                                                                                         >  2:579704/1‑140 (MQ=255)

 tctcGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCgg                                                                                                                        >  1:1369776/1‑140 (MQ=255)

  ctcGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGc                                                                                                                       <  1:631428/140‑1 (MQ=255)

  ctcGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGc                                                                                                                       <  2:1676160/140‑1 (MQ=255)

  ctcGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGc                                                                                                                       >  2:150803/1‑140 (MQ=255)

  ctcGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGc                                                                                                                       <  1:690311/140‑1 (MQ=255)

   tcGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCa                                                                                                                      >  2:521420/1‑140 (MQ=255)

       cGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGgtc                                                                                                                  <  1:579704/140‑1 (MQ=255)

          gATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGgtcgtc                                                                                                               >  2:1410263/1‑140 (MQ=255)

          gATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGgtcgtc                                                                                                               >  1:1681093/1‑140 (MQ=255)

           aTCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCa                                                                                                              >  1:277558/1‑140 (MQ=255)

               aaGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTg                                                                                                          <  2:1122847/140‑1 (MQ=255)

                    tCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTc                                                                                                     <  2:450103/140‑1 (MQ=255)

                    tCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTc                                                                                                     <  2:590411/140‑1 (MQ=255)

                    tCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTc                                                                                                     >  1:203889/1‑140 (MQ=255)

                     cTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTcc                                                                                                    >  1:811226/1‑140 (MQ=255)

                      tGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCa                                                                                                   >  2:647729/1‑140 (MQ=255)

                      tGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCa                                                                                                   >  1:295098/1‑140 (MQ=255)

                         aTTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAgcg                                                                                                <  1:263582/140‑1 (MQ=255)

                         aTTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAgcg                                                                                                <  2:1596436/140‑1 (MQ=255)

                         aTTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAgcg                                                                                                <  2:288659/140‑1 (MQ=255)

                         aTTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAgcg                                                                                                <  2:1369776/140‑1 (MQ=255)

                           tCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCt                                                                                              <  2:1710912/140‑1 (MQ=255)

                           tCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCt                                                                                              <  2:203889/140‑1 (MQ=255)

                           tCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCt                                                                                              <  2:1255184/140‑1 (MQ=255)

                           tCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCt                                                                                              <  1:720978/140‑1 (MQ=255)

                            cAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTc                                                                                             >  2:44099/1‑140 (MQ=255)

                             aGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCa                                                                                            >  2:1279326/1‑140 (MQ=255)

                                gATATCCTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTa                                                                                         <  1:1557905/140‑1 (MQ=255)

                                  tatCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTa                                                                                         <  1:456426/138‑1 (MQ=255)

                                  tatCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTa                                                                                         >  2:456426/1‑138 (MQ=255)

                                        aCCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTg                                                                                 <  2:621403/140‑1 (MQ=255)

                                            tCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACa                                                                             <  1:1466900/140‑1 (MQ=255)

                                             ccTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACAt                                                                            <  2:9846/140‑1 (MQ=255)

                                             ccTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACAt                                                                            <  2:1681093/140‑1 (MQ=255)

                                             ccTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACAt                                                                            <  1:647729/140‑1 (MQ=255)

                                              cTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCAc                                                                                   >  2:270407/1‑132 (MQ=255)

                                              cTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCAc                                                                                   <  1:270407/132‑1 (MQ=255)

                                                  gcgcAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGtt                                                                       <  1:1247433/140‑1 (MQ=255)

                                                      aGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTaa                                                                   <  2:811226/140‑1 (MQ=255)

                                                      aGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAAGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTaa                                                                   >  2:195346/1‑140 (MQ=255)

                                                      aGCGTTTACCGCTAGCCGAAAAAGGTCTGGCGTGGTTAATGCCAACCGTGGGGATGGTGGTTCTGGCCTTTATCTGTTCTCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGACCATTTGTTTTaa                                                                   >  2:693713/1‑140 (MQ=255)

                                                        cGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAAcc                                                                 >  1:1593315/1‑140 (MQ=255)

                                                         gTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCa                                                                <  1:521420/140‑1 (MQ=255)

                                                          tttACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAAGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCAc                                                               <  1:195346/140‑1 (MQ=255)

                                                              ccGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACgggg                                                           <  1:1407304/140‑1 (MQ=255)

                                                                   ggCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCg                                                      <  2:1212451/140‑1 (MQ=255)

                                                                    gCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGa                                                     >  2:278137/1‑140 (MQ=255)

                                                                    gCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGa                                                     >  2:1409443/1‑140 (MQ=255)

                                                                      cGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATg                                                   >  2:5910/1‑140 (MQ=255)

                                                                        aaCAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcc                                                 <  2:277558/140‑1 (MQ=255)

                                                                           aaGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCg                                              <  2:17929/140‑1 (MQ=255)

                                                                             ggTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTg                                            <  1:1410263/140‑1 (MQ=255)

                                                                                  ggCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGtttt                                       >  1:593349/1‑140 (MQ=255)

                                                                                   gCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGCACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGttttt                                      >  1:992768/1‑140 (MQ=255)

                                                                                   gCGTGGTTAATGCCAACAGTGGTGATAGTGGTTCTGGCCATTATCTGGTATCGCGCGGCATGTCGTCAGGTGACCTCCAGCGCTCACTAAATTATTTAACATTTGTTTTAACTATGGGGCTGCGATGCCCCGTGGttttt                                      >  2:616661/1‑140 (MQ=255)

                                                                                       ggTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                  <  2:43/140‑1 (MQ=255)

                                                                                       ggTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAtt                                  <  1:44099/140‑1 (MQ=255)

                                                                                        gTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAttt                                 >  2:69302/1‑139 (MQ=255)

                                                                                        gTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATtg                                 >  1:1210182/1‑140 (MQ=255)

                                                                                        gTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATtg                                 >  2:1648177/1‑140 (MQ=255)

                                                                                             tGCCAACAGTGGTGATTGTGGTTCTGGCCATTATCTGGCATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTg                            >  1:390924/1‑140 (MQ=255)

                                                                                             tGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTg                            >  1:1466046/1‑140 (MQ=255)

                                                                                              gCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAGCATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGa                           <  1:1648177/140‑1 (MQ=255)

                                                                                              gCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGa                           <  1:150803/140‑1 (MQ=255)

                                                                                               ccAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAt                          <  2:1436446/140‑1 (MQ=255)

                                                                                               ccAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTCGAt                          <  2:295098/140‑1 (MQ=255)

                                                                                                   cAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGTTGGGt                      >  1:1459939/1‑140 (MQ=255)

                                                                                                   cAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt                      >  1:1294871/1‑140 (MQ=255)

                                                                                                   cAGTGGTGATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt                      >  2:133944/1‑140 (MQ=255)

                                                                                                       ggtgATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAgg                  <  2:1294871/140‑1 (MQ=255)

                                                                                                        gtgATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGa                 >  2:381048/1‑140 (MQ=255)

                                                                                                         tgATGGTGGTTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGaa                <  1:5910/140‑1 (MQ=255)

                                                                                                             ggtggtTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGa            <  1:669902/140‑1 (MQ=255)

                                                                                                                ggtTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATgg         <  1:1279326/140‑1 (MQ=255)

                                                                                                                 gtTCTGGCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGa        <  1:1409443/140‑1 (MQ=255)

                                                                                                                       gCCATTATCTGGTATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTaa  >  2:1473582/1‑140 (MQ=255)

TTCTCGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAA  >  NC_000913/420738‑420996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A