Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	298,595	C→A	V44V (GTG→GTT)	paoD ←	molybdenum cofactor insertion chaperone for PaoABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	298,595	0	C	A	100.0%	162.0 / NA	46	V44V (GTG→GTT)	paoD	molybdenum cofactor insertion chaperone for PaoABC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/25); total (21/25)

TCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGACATTGTTC  >  NC_000913/298459‑298731

tcatcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAAcc                                                                                                                                       >  1:563971/1‑140 (MQ=255)

  atcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAg                                                                                                                                     <  1:383268/140‑1 (MQ=255)

    ctcCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCa                                                                                                                                   >  1:4973/1‑140 (MQ=255)

       cAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCg                                                                                                                                <  2:1321600/140‑1 (MQ=255)

       cAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCg                                                                                                                                >  1:1249745/1‑140 (MQ=255)

          cgcTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTc                                                                                                                             >  2:240336/1‑140 (MQ=255)

          cgcTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTc                                                                                                                             >  2:598481/1‑140 (MQ=255)

          cgcTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTc                                                                                                                             <  1:805993/140‑1 (MQ=255)

            cTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCg                                                                                                                                <  1:706994/135‑1 (MQ=255)

            cTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCg                                                                                                                                >  2:706994/1‑135 (MQ=255)

            cTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCt                                                                                                                           >  2:549285/1‑140 (MQ=255)

              tCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCttt                                                                                                                         >  1:746638/1‑140 (MQ=255)

              tCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCttt                                                                                                                         >  1:1265243/1‑140 (MQ=255)

                   agcggcagcggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCgg                                                                                                                    <  1:497444/140‑1 (MQ=255)

                       gcagcggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACAt                                                                                                                >  1:154060/1‑140 (MQ=255)

                          gcggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAg                                                                                                             <  2:1265243/140‑1 (MQ=255)

                                     gCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACgg                                                                                                  >  1:485662/1‑140 (MQ=255)

                                         ccgccAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGaa                                                                                              >  2:547970/1‑140 (MQ=255)

                                            ccAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAgcg                                                                                           >  2:1237326/1‑140 (MQ=255)

                                                     aaaCCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGt                                                                                  <  1:556416/140‑1 (MQ=255)

                                                         cGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCgg                                                                              <  2:190588/140‑1 (MQ=255)

                                                          gCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCggt                                                                             >  2:709286/1‑140 (MQ=255)

                                                             gTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCggtggt                                                                          <  2:563971/140‑1 (MQ=255)

                                                               aaCGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCAGCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCa                                                                        <  1:424174/140‑1 (MQ=255)

                                                                 cGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCACCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATc                                                                      <  2:934993/140‑1 (MQ=255)

                                                                  gACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCg                                                                     <  1:1250582/140‑1 (MQ=255)

                                                                        cTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTgaga                                                               <  2:756370/140‑1 (MQ=255)

                                                                           cgcgCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAAc                                                            >  1:1024615/1‑140 (MQ=255)

                                                                             cgcACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCTCCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGc                                                          <  1:859338/140‑1 (MQ=255)

                                                                             cgcACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGc                                                          <  2:1253825/140‑1 (MQ=255)

                                                                                   cccaTCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGctc                                                    <  1:744294/139‑1 (MQ=255)

                                                                                   accaTCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGctc                                                    <  2:56434/140‑1 (MQ=255)

                                                                                   accaTCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGctc                                                    <  1:60986/140‑1 (MQ=255)

                                                                                            gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                           >  2:1320652/1‑140 (MQ=255)

                                                                                            gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                           >  2:960731/1‑140 (MQ=255)

                                                                                             cgccGAGCGGGCGCGCCGCGTCGCCGCGTATCTCCACCAGCGCAAACAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAACGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGc                                          <  2:781959/138‑1 (MQ=255)

                                                                                             ccccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGc                                          <  1:329884/140‑1 (MQ=255)

                                                                                               ccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCa                                        <  2:708797/140‑1 (MQ=255)

                                                                                                  aGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAgtg                                     >  1:778799/1‑140 (MQ=255)

                                                                                                      ggCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTAATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCg                                 >  1:514386/1‑140 (MQ=255)

                                                                                                         gcgccgcgccGCCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCt                              <  2:369335/140‑1 (MQ=255)

                                                                                                            ccgcgccgcCGCGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTg                           >  1:801276/1‑140 (MQ=255)

                                                                                                               cgccgccgcGTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCa                        <  1:240336/140‑1 (MQ=255)

                                                                                                                     cgcgTATCTCCACCAGCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGc                  <  1:1320652/140‑1 (MQ=255)

                                                                                                                                    gCGTAACCAGCACCGCTCCTTTACTGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGACATTGtt   >  1:1225282/1‑140 (MQ=255)

                                                                                                                                    gCGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGTTATGACATTGtt   <  1:1237326/140‑1 (MQ=255)

                                                                                                                                     cGTAACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGACATTGTtc  <  1:151958/140‑1 (MQ=255)

TCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGACATTGTTC  >  NC_000913/298459‑298731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A