Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,730	C→A	W159L (TGG→TTG)	ecpE ←	putative fimbrial chaperone EcpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,730	0	C	A	99.1%	380.0 / ‑3.1	106	W159L (TGG→TTG)	ecpE	putative fimbrial chaperone EcpE
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (60/45); total (60/46)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

Only 100 of 108 total aligned reads displayed.

GCTGATGAACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCC  >  NC_000913/304593‑304862

gCTGATGAACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAAt                                                                                                                                    >  2:851122/1‑140 (MQ=255)

gCTGATGAACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAAt                                                                                                                                    >  2:1358673/1‑140 (MQ=255)

 cTGATGAACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAAtt                                                                                                                                   >  1:909946/1‑140 (MQ=255)

    atgaACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTaa                                                                                                                                >  1:421595/1‑140 (MQ=255)

    atgaACCACGTCTCCCGGACGTAGATACCAGACATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTaa                                                                                                                                >  2:935636/1‑140 (MQ=255)

     tgaACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATTCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTaaa                                                                                                                               >  2:1088777/1‑140 (MQ=255)

      gaACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAAc                                                                                                                              <  1:1088777/140‑1 (MQ=255)

        aCCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTg                                                                                                                            <  1:179021/140‑1 (MQ=255)

              tctcCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTg                                                                                                                      >  2:1065030/1‑140 (MQ=255)

              tctcCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTg                                                                                                                      >  1:54817/1‑140 (MQ=255)

               ctcCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGa                                                                                                                     <  1:910657/140‑1 (MQ=255)

                 cccGGACGTAGATACCAGGCATCTCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACg                                                                                                                   <  2:818629/140‑1 (MQ=255)

                 cccGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACg                                                                                                                   >  2:1239379/1‑140 (MQ=255)

                  ccGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGt                                                                                                                  >  2:1076972/1‑140 (MQ=255)

                      aCGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTc                                                                                                              >  1:18756/1‑140 (MQ=255)

                       cGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTct                                                                                                             >  2:1208859/1‑139 (MQ=255)

                       cGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCg                                                                                                             <  2:992328/140‑1 (MQ=255)

                         tAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCACGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGta                                                                                                           <  1:489266/140‑1 (MQ=255)

                          aGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGtac                                                                                                          <  2:712104/140‑1 (MQ=255)

                          aGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGtac                                                                                                          <  1:841711/140‑1 (MQ=255)

                          aGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGtac                                                                                                          <  2:909946/140‑1 (MQ=255)

                           gATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTg                                                                                                                 <  1:478955/132‑1 (MQ=255)

                           gATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTg                                                                                                                 >  2:478955/1‑132 (MQ=255)

                            aTACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGtacta                                                                                                        <  2:421595/140‑1 (MQ=255)

                             tACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGtactac                                                                                                       <  1:1313161/140‑1 (MQ=255)

                                cAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTTTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAg                                                                                                    >  1:244911/1‑140 (MQ=255)

                                 aGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGa                                                                                                   >  2:1133936/1‑140 (MQ=255)

                                 aGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGa                                                                                                   <  2:1196901/140‑1 (MQ=255)

                                   gCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAAt                                                                                                 >  2:360851/1‑140 (MQ=255)

                                    cATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATg                                                                                                >  2:941683/1‑140 (MQ=255)

                                     aTCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGtac                                                                                                          >  1:995284/1‑129 (MQ=255)

                                     aTCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATgg                                                                                               >  1:522064/1‑140 (MQ=255)

                                     aTCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATgg                                                                                               <  2:1120461/140‑1 (MQ=255)

                                     aTCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATgg                                                                                               <  2:192783/140‑1 (MQ=255)

                                      tCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGt                                                                                              >  1:317523/1‑140 (MQ=255)

                                       cGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTa                                                                                             >  1:1066459/1‑140 (MQ=255)

                                       cGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTa                                                                                             >  2:1132437/1‑140 (MQ=255)

                                         ccTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGTTATc                                                                                           >  2:1120776/1‑140 (MQ=255)

                                             ccTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATc                                                                                       >  1:1373437/1‑140 (MQ=255)

                                             ccTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATc                                                                                       >  1:675340/1‑140 (MQ=255)

                                                ctTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATcacc                                                                                    <  2:18756/140‑1 (MQ=255)

                                                     gTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATcaccaccac                                                                               <  2:1172925/140‑1 (MQ=255)

                                                     gTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATcaccaccac                                                                               <  2:1101542/140‑1 (MQ=255)

                                                     gTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATcaccaccac                                                                               <  1:975804/140‑1 (MQ=255)

                                                      tCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCaccaccacc                                                                              >  2:771633/1‑140 (MQ=255)

                                                         aaTCACATCCTGGCTTAATCAGTAGCTTAAACCATATGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGc                                                                           >  1:880232/1‑140 (MQ=255)

                                                          aTCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                                                          <  1:851122/140‑1 (MQ=255)

                                                             acaTCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGAATCCATCACCACCACCGGCTcct                                                                       >  2:819530/1‑139 (MQ=255)

                                                               aTCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTg                                                                     <  1:506621/140‑1 (MQ=255)

                                                                  cTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTg                                                                  <  1:1208859/140‑1 (MQ=255)

                                                                         aaTCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgc                                                           >  2:826908/1‑140 (MQ=255)

                                                                         aaTCAGTAGCTTAAACCATGTGTTGCCAGAGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgc                                                           >  1:171776/1‑140 (MQ=255)

                                                                          aTCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                                                          >  1:751168/1‑140 (MQ=255)

                                                                          aTCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                                                          <  1:1228872/140‑1 (MQ=255)

                                                                               tAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCgg                                                     >  2:380427/1‑140 (MQ=255)

                                                                               tAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCgg                                                     >  2:949646/1‑140 (MQ=255)

                                                                                 gCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGtt                                                   <  2:13077/140‑1 (MQ=255)

                                                                                    tAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTggg                                                >  2:1155630/1‑140 (MQ=255)

                                                                                      aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt                                              <  2:244911/140‑1 (MQ=255)

                                                                                       aCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTg                                             >  2:378142/1‑140 (MQ=255)

                                                                                        ccATATGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc                                            <  2:880232/140‑1 (MQ=255)

                                                                                         cATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCg                                           <  1:378142/140‑1 (MQ=255)

                                                                                         cATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCg                                           <  2:1373437/140‑1 (MQ=255)

                                                                                         cATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTAAGGCTGCg                                           <  2:331698/140‑1 (MQ=255)

                                                                                           tgtgTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTc                                         >  1:515396/1‑140 (MQ=255)

                                                                                           tgtgTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTc                                         >  1:299445/1‑140 (MQ=255)

                                                                                           tgtgTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTc                                         >  1:1032910/1‑140 (MQ=255)

                                                                                           tgtgTTGCCAGTGTTACTGACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTc                                         >  1:1245469/1‑140 (MQ=255)

                                                                                            gtgtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                        >  2:171599/1‑140 (MQ=255)

                                                                                            gtgtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                        >  1:538039/1‑140 (MQ=255)

                                                                                            gtgtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                        >  2:511080/1‑140 (MQ=255)

                                                                                               ttGCCAGTGTTACTTACCGTTCCTGTCCCCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTTCTACCAGAATGGTATCCATCTCCCCCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGt                                     <  1:1358673/140‑1 (MQ=255)

                                                                                               ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGt                                     <  1:1098574/140‑1 (MQ=255)

                                                                                               ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGt                                     <  2:317523/140‑1 (MQ=255)

                                                                                                tGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTa                                    <  2:751168/140‑1 (MQ=255)

                                                                                                tGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTa                                    <  1:182698/140‑1 (MQ=255)

                                                                                                 gcgaGTGTTATTTACCGTTCCTGTTACCTTGTCGGAGGACAATTTAAACTGAACCTGACGTGGCCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGGTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt                                   >  2:1001147/4‑140 (MQ=255)

                                                                                                 gCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt                                   >  2:53569/1‑140 (MQ=255)

                                                                                                 gCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt                                   >  2:635444/1‑140 (MQ=255)

                                                                                                     gtgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGtt                               >  2:1368101/1‑140 (MQ=255)

                                                                                                      tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTa                              <  1:826908/140‑1 (MQ=255)

                                                                                                      tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTa                              <  1:890403/140‑1 (MQ=255)

                                                                                                      tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTa                              <  1:1239379/140‑1 (MQ=255)

                                                                                                      tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCAGGTTGGGCTGCGTCTTGTATGGTTa                              >  1:1012585/1‑140 (MQ=255)

                                                                                                         tacttacCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGa                           >  1:1006912/1‑140 (MQ=255)

                                                                                                                  ttCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAcc                  >  1:694716/1‑140 (MQ=255)

                                                                                                                    ccTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                >  1:873487/1‑140 (MQ=255)

                                                                                                                    ccTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                >  1:684349/1‑140 (MQ=255)

                                                                                                                    ccTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                >  2:950805/1‑140 (MQ=255)

                                                                                                                        tCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGa            >  2:1021539/1‑140 (MQ=255)

                                                                                                                        tCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGa            >  2:1292108/1‑140 (MQ=255)

                                                                                                                          aCCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGaaa          >  1:1021015/1‑140 (MQ=255)

                                                                                                                             ttGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATga       <  1:511080/140‑1 (MQ=255)

                                                                                                                              tGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATgag      >  2:911785/1‑140 (MQ=255)

                                                                                                                               gTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATgaga     <  1:535715/140‑1 (MQ=255)

                                                                                                                               gTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATgaga     <  1:171599/140‑1 (MQ=255)

                                                                                                                               gTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATgaga     >  2:227905/1‑140 (MQ=255)

                                                                                                                                 cGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGAcc   <  2:445720/140‑1 (MQ=255)

                                                                                                                                 cGAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGAcc   <  2:442913/140‑1 (MQ=255)

                                                                                                                                  gAAGGACAATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGAccc  >  1:1205922/1‑140 (MQ=255)

GCTGATGAACCACGTCTCCCGGACGTAGATACCAGGCATCGCCTTCCTCTTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCC  >  NC_000913/304593‑304862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A