Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	311,086	G→T	A84E (GCG→GAG)	ecpR ←	DNA‑binding transcriptional dual regulator MatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	311,086	0	G	T	100.0%	413.8 / NA	115	A84E (GCG→GAG)	ecpR	DNA‑binding transcriptional dual regulator MatA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (68/47); total (68/47)

Only 100 of 116 total aligned reads displayed.

CATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATA  >  NC_000913/310949‑311221

  tAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGATTCCCCTTTACCTCGGa                                                                                                                                     <  1:329281/140‑1 (MQ=255)

     aCCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATaa                                                                                                                                  >  2:824590/1‑140 (MQ=255)

       cAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAAcc                                                                                                                                >  2:764864/1‑140 (MQ=255)

       cAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAAcc                                                                                                                                >  1:507356/1‑140 (MQ=255)

        aGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACca                                                                                                                               <  1:735143/140‑1 (MQ=255)

        aGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACca                                                                                                                               >  2:93991/1‑140 (MQ=255)

          ttCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACcatt                                                                                                                             <  2:46514/140‑1 (MQ=255)

           tCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACcattc                                                                                                                            >  2:286130/1‑140 (MQ=255)

               ttCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTa                                                                                                                        >  1:284066/1‑140 (MQ=255)

               ttCTAATATCACGACTCAGTCCAGCGCATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTa                                                                                                                        >  2:957826/1‑140 (MQ=255)

                  tAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCa                                                                                                                     <  2:1051167/140‑1 (MQ=255)

                    atatCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACg                                                                                                                   >  1:593579/1‑140 (MQ=255)

                    atatCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACg                                                                                                                   >  1:1245008/1‑140 (MQ=255)

                    atatCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACg                                                                                                                   >  2:1285968/1‑140 (MQ=255)

                     tatCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACgg                                                                                                                  <  1:1078278/140‑1 (MQ=255)

                         aCGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTta                                                                                                              >  1:1237046/1‑140 (MQ=255)

                         aCGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTta                                                                                                              <  2:1177091/140‑1 (MQ=255)

                            aCTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATaa                                                                                                           >  1:717470/1‑140 (MQ=255)

                               cAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTTCCTCGGATAACCATTCATTATCACGGTTTATAAAtt                                                                                                        <  1:30738/140‑1 (MQ=255)

                                aGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTc                                                                                                       >  1:222361/1‑140 (MQ=255)

                                aGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTc                                                                                                       >  2:731405/1‑140 (MQ=255)

                                      gCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATaa                                                                                                 <  1:798824/140‑1 (MQ=255)

                                       cGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATaaa                                                                                                >  2:444417/1‑140 (MQ=255)

                                        gTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAg                                                                                               <  1:731405/140‑1 (MQ=255)

                                           taCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAAt                                                                                            <  2:1020226/140‑1 (MQ=255)

                                           taCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAAt                                                                                            <  1:957826/140‑1 (MQ=255)

                                           taCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAAt                                                                                            <  2:1245008/140‑1 (MQ=255)

                                           taCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAAt                                                                                            <  2:284066/140‑1 (MQ=255)

                                           aaCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAAt                                                                                            <  2:15980/139‑1 (MQ=255)

                                            aCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATaa                                                                                                 <  1:797850/134‑1 (MQ=255)

                                            aCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATc                                                                                           >  1:499518/1‑140 (MQ=255)

                                            aCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATc                                                                                           >  2:560766/1‑140 (MQ=255)

                                                cGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTa                                                                                       >  1:800806/1‑140 (MQ=255)

                                                cGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTa                                                                                       >  2:487865/1‑140 (MQ=255)

                                                 gCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAg                                                                                               <  1:97795/131‑1 (MQ=255)

                                                 gCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAg                                                                                               >  2:97795/1‑131 (MQ=255)

                                                   cccTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTg                                                                                    >  2:188292/1‑140 (MQ=255)

                                                   cccTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTg                                                                                    >  1:302846/1‑140 (MQ=255)

                                                      tAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTgaga                                                                                 >  1:1050521/1‑140 (MQ=255)

                                                      tAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTgaga                                                                                 <  1:176579/140‑1 (MQ=255)

                                                            tGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATa                                                                           <  2:1237046/140‑1 (MQ=255)

                                                             gCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATaa                                                                          >  2:735154/1‑140 (MQ=255)

                                                             gCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATaa                                                                          >  2:119311/1‑140 (MQ=255)

                                                               tATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAac                                                                        >  1:990294/1‑140 (MQ=255)

                                                                 ttGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAacac                                                                      >  1:522860/1‑140 (MQ=255)

                                                                  tGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACg                                                                     >  2:292611/1‑140 (MQ=255)

                                                                    tAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGaa                                                                   <  1:1285968/140‑1 (MQ=255)

                                                                      aTACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCTGTACTGAGATTGATAAACACGAAAt                                                                 >  1:562747/1‑140 (MQ=255)

                                                                      aTACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAAt                                                                 >  2:233494/1‑140 (MQ=255)

                                                                         ccAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCtt                                                              >  1:594246/1‑140 (MQ=255)

                                                                           aataatTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCtttt                                                            >  2:889125/1‑140 (MQ=255)

                                                                            ataatTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCttttt                                                           >  2:760769/1‑139 (MQ=255)

                                                                            ataatTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTc                                                           >  1:795281/1‑140 (MQ=255)

                                                                            ataatTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTc                                                           >  1:147131/1‑140 (MQ=255)

                                                                              aatTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTa                                                         >  1:725351/1‑140 (MQ=255)

                                                                                  tGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAAttt                                                     >  1:470481/1‑140 (MQ=255)

                                                                                  tGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAAttt                                                     >  2:226947/1‑140 (MQ=255)

                                                                                   gCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAAtttt                                                    >  1:947434/1‑140 (MQ=255)

                                                                                    cTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAAttttt                                                   >  2:533033/1‑139 (MQ=255)

                                                                                    cTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTtc                                                   >  1:454499/1‑140 (MQ=255)

                                                                                    cTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTtc                                                   >  1:157370/1‑140 (MQ=255)

                                                                                       aGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTcc                                                >  2:488596/1‑140 (MQ=255)

                                                                                               gACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACgatag                                        <  2:604829/140‑1 (MQ=255)

                                                                                               gACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACgatag                                        <  1:292611/140‑1 (MQ=255)

                                                                                               gACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACgatag                                        <  2:1246626/140‑1 (MQ=255)

                                                                                               gACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACgatag                                        <  1:829905/140‑1 (MQ=255)

                                                                                               gACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACgatag                                        <  1:950288/140‑1 (MQ=255)

                                                                                                   ttCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATCACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAAACTTTTCTAATTTTCTCCAACgatag                                        <  1:1183040/136‑1 (MQ=255)

                                                                                                   ttCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACgatag                                        >  2:1183040/1‑136 (MQ=255)

                                                                                                    tCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATaaa                                   >  1:409087/1‑140 (MQ=255)

                                                                                                     cTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGATTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAAt                                  >  2:395981/1‑140 (MQ=255)

                                                                                                        gCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTat                               >  2:377798/1‑139 (MQ=255)

                                                                                                        gCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTaa                               >  1:399500/1‑140 (MQ=255)

                                                                                                        gCCGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTaa                               >  1:1313933/1‑140 (MQ=255)

                                                                                                         ccGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAAt                              <  1:281498/140‑1 (MQ=255)

                                                                                                          cGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATc                             <  1:889125/140‑1 (MQ=255)

                                                                                                          cGCAATCAATACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATc                             >  1:939769/1‑140 (MQ=255)

                                                                                                                caatACGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGa                       <  2:776018/140‑1 (MQ=255)

                                                                                                                     cGACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATcc                  <  2:399500/140‑1 (MQ=255)

                                                                                                                     agACCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATcc                  <  2:391568/139‑1 (MQ=255)

                                                                                                                       aCCTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCcac                >  2:460506/1‑140 (MQ=255)

                                                                                                                        acTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCcaca               <  2:470481/139‑1 (MQ=255)

                                                                                                                         cTGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACaa              >  1:872120/1‑140 (MQ=255)

                                                                                                                          tGTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAAt             <  1:226947/140‑1 (MQ=255)

                                                                                                                           gTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATc            <  2:499518/140‑1 (MQ=255)

                                                                                                                           gTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATc            <  1:764864/140‑1 (MQ=255)

                                                                                                                           gTTTCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATc            >  1:1161872/1‑140 (MQ=255)

                                                                                                                            tttCCCCTTTACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCa           >  2:411099/1‑140 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    >  1:702319/1‑140 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  2:947434/140‑1 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  1:513412/140‑1 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  1:1199839/140‑1 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  2:795281/140‑1 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  2:717470/140‑1 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  2:594246/140‑1 (MQ=255)

                                                                                                                                   tttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGa    <  1:188292/140‑1 (MQ=255)

                                                                                                                                    ttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGAt   >  2:1194444/1‑140 (MQ=255)

                                                                                                                                    ttACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAACCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGAt   >  2:380577/1‑140 (MQ=255)

                                                                                                                                     tACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATa  <  2:157370/140‑1 (MQ=255)

                                                                                                                                     tACCTCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATa  <  2:634817/140‑1 (MQ=255)

CATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATA  >  NC_000913/310949‑311221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A