Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F48 I1 R1	55	131.7	4456318	99.2%	4420667	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,789	G→A	intergenic (‑12/+352)	ykgM ← / ← ykgR	putative ribosomal protein/putative membrane protein YkgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,789	0	G	A	99.4%	561.1 / ‑4.6	158	intergenic (‑12/+352)	ykgM/ykgR	putative ribosomal protein/putative membrane protein YkgR
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (86/71); total (87/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 177 total aligned reads displayed.

ATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCA  >  NC_000913/312652‑312925

atccGTTACCCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtatt                                                                                                                                        <  1:1131522/137‑1 (MQ=255)

aTACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtatt                                                                                                                                        >  1:1989077/1‑140 (MQ=255)

aTACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtatt                                                                                                                                        <  2:341320/140‑1 (MQ=255)

aTACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtatt                                                                                                                                        >  1:1167619/1‑140 (MQ=255)

 tACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtatta                                                                                                                                       <  2:1167619/140‑1 (MQ=255)

    gTTGCGACATCCAGCTCAATTTCACAGTCTGTTTTGATGGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtattatgt                                                                                                                                    <  2:1837280/140‑1 (MQ=255)

    gTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtattatgt                                                                                                                                    <  1:219178/140‑1 (MQ=255)

      tACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATATTTACCtattatgttt                                                                                                                                  >  2:1764379/1‑139 (MQ=255)

       aCGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtatt                                                                                                                                        >  2:50675/1‑133 (MQ=255)

        cGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTtata                                                                                                                                >  2:334234/1‑140 (MQ=255)

        cGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTtata                                                                                                                                >  1:1515493/1‑140 (MQ=255)

         gCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCtattatgt                                                                                                                                    <  2:324010/135‑1 (MQ=255)

         gCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataa                                                                                                                               >  1:1540463/1‑140 (MQ=255)

            aTCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacat                                                                                                                            <  2:1891623/140‑1 (MQ=255)

            aTCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacat                                                                                                                            <  1:1146770/140‑1 (MQ=255)

            aTCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacat                                                                                                                            <  2:1322786/140‑1 (MQ=255)

            aTCCAGCTAAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacat                                                                                                                            <  2:661854/140‑1 (MQ=255)

             tCCAGCTCGATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacata                                                                                                                           >  1:1873858/1‑140 (MQ=255)

             tCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacata                                                                                                                           <  2:1378989/140‑1 (MQ=255)

             tCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacata                                                                                                                           >  1:2211716/1‑140 (MQ=255)

             tCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacata                                                                                                                           >  1:337348/1‑140 (MQ=255)

              ccAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTataacataa                                                                                                                          >  2:1397642/1‑140 (MQ=255)

                aGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAAcc                                                                                                                        <  1:1633081/140‑1 (MQ=255)

                  cTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCAt                                                                                                                      >  1:2009811/1‑140 (MQ=255)

                   tCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATa                                                                                                                     >  2:1622407/1‑140 (MQ=255)

                   tCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATa                                                                                                                     <  1:812507/140‑1 (MQ=255)

                   tCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACACAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATa                                                                                                                     <  2:842575/140‑1 (MQ=255)

                    cAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATaa                                                                                                                    <  2:541112/140‑1 (MQ=255)

                     aaTCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATaaa                                                                                                                   >  1:1745007/1‑140 (MQ=255)

                        gcagctgTCTTTATTCATAGCCGAGCCAATTTTAAAGTACTCATAAACACCGGTGTCGTGAAACACCAAAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTTCCTATTATGTTATAACATAACCATAAAGAt                                                                                                                <  2:1456542/134‑1 (MQ=255)

                         tcACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATa                                                                                                               >  1:569271/1‑140 (MQ=255)

                             ggTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATg                                                                                                           <  1:1622407/140‑1 (MQ=255)

                             ggTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATg                                                                                                           <  2:22267/140‑1 (MQ=255)

                               tCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCt                                                                                                         >  1:2137445/1‑140 (MQ=255)

                               tCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCt                                                                                                         >  1:1577105/1‑140 (MQ=255)

                                   ttttGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATc                                                                                                     >  1:1532317/1‑140 (MQ=255)

                                       gATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGa                                                                                                 <  1:597177/140‑1 (MQ=255)

                                        aTAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGAt                                                                                                <  1:506876/140‑1 (MQ=255)

                                        aTAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGAt                                                                                                <  2:1577105/140‑1 (MQ=255)

                                        aTAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGAt                                                                                                <  1:907703/140‑1 (MQ=255)

                                         tAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATc                                                                                               >  2:805532/1‑140 (MQ=255)

                                          aGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCa                                                                                              <  2:1056007/140‑1 (MQ=255)

                                            tCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCAtt                                                                                            >  1:304108/1‑140 (MQ=255)

                                             cGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCAttt                                                                                           >  1:1330558/1‑140 (MQ=255)

                                              gAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTc                                                                                          <  1:1397642/140‑1 (MQ=255)

                                               aGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCa                                                                                         <  1:741280/140‑1 (MQ=255)

                                                gCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCaa                                                                                        <  1:714389/140‑1 (MQ=255)

                                                      tttAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCa                                                                                  <  1:305438/140‑1 (MQ=255)

                                                             tACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCtg                                                                           <  2:905913/140‑1 (MQ=255)

                                                               cTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCtgtt                                                                         >  2:151784/1‑139 (MQ=255)

                                                               cTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCtgtg                                                                         >  1:627431/1‑140 (MQ=255)

                                                               cTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCtgtg                                                                         >  2:1420929/1‑140 (MQ=255)

                                                                tcatcaACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGa                                                                        <  2:304108/140‑1 (MQ=255)

                                                                tcatcaACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGa                                                                        <  1:662153/140‑1 (MQ=255)

                                                                tcatcaACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGa                                                                        <  2:1989077/140‑1 (MQ=255)

                                                                  atcaACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGAct                                                                      >  2:704639/1‑140 (MQ=255)

                                                                          tGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtgg                                                              >  2:548571/1‑140 (MQ=255)

                                                                          tGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTGtgtgg                                                              >  2:1360010/1‑140 (MQ=255)

                                                                            gtgtCGTGTAACACCACAGTACGATACACAGGATGGATATTGGGCTTCATAATTTTTACCTATTATGTTATAACATAACCGTAAAGATACATGTTCAACCTGATCATTTTAACATTCAATCCCTGTGACTCTTtttggat                                                            >  2:626424/1‑140 (MQ=255)

                                                                            gtgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggat                                                            >  2:2112897/1‑140 (MQ=255)

                                                                            gtgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggat                                                            >  2:204277/1‑140 (MQ=255)

                                                                            gtgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggat                                                            >  1:755980/1‑140 (MQ=255)

                                                                            gtgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggat                                                            >  1:152201/1‑140 (MQ=255)

                                                                                 gTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggatgtgga                                                       >  2:680140/1‑140 (MQ=255)

                                                                                 gTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtggatgtgga                                                       >  2:942255/1‑140 (MQ=255)

                                                                                        accacAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATACCCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCg                                                <  1:266421/140‑1 (MQ=255)

                                                                                          cacaGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCa                                              >  2:1012596/1‑140 (MQ=255)

                                                                                          cacaGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCa                                              <  1:180579/140‑1 (MQ=255)

                                                                                              gTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACgg                                          >  2:670543/1‑140 (MQ=255)

                                                                                               tACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACggg                                         <  2:152201/140‑1 (MQ=255)

                                                                                               tACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGATATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTATGGATGTGGAATTTTCGCAACggt                                         >  2:559516/1‑139 (MQ=255)

                                                                                                 cGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGa                                       >  2:1946947/1‑140 (MQ=255)

                                                                                                    tACTCAGGTTGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAAct                                    >  2:987783/1‑140 (MQ=255)

                                                                                                    tACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAAct                                    >  1:54267/1‑140 (MQ=255)

                                                                                                    tACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAAct                                    >  2:470081/1‑140 (MQ=255)

                                                                                                    tACTCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAAct                                    <  2:1551031/140‑1 (MQ=255)

                                                                                                       tCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTg                                 >  1:1002396/1‑140 (MQ=255)

                                                                                                       tCAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTg                                 >  1:1473375/1‑140 (MQ=255)

                                                                                                       acAGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTg                                 <  2:1305078/139‑1 (MQ=255)

                                                                                                         aGGATGGATATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGc                               >  2:792648/1‑140 (MQ=255)

                                                                                                           gatggatATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCAt                             <  1:1012596/140‑1 (MQ=255)

                                                                                                           gatggatATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCAt                             >  1:144548/1‑140 (MQ=255)

                                                                                                           gatggatATTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCAt                             >  2:1613117/1‑140 (MQ=255)

                                                                                                                atatTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTa                        >  1:405254/1‑140 (MQ=255)

                                                                                                                atatTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTa                        >  2:260434/1‑140 (MQ=255)

                                                                                                                 tatTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTag                       >  1:2092961/1‑140 (MQ=255)

                                                                                                                 tatTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTag                       <  1:1727605/140‑1 (MQ=255)

                                                                                                                  atTGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTagc                      <  1:673451/140‑1 (MQ=255)

                                                                                                                   ttGGGCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTagca                     >  2:1905696/1‑140 (MQ=255)

                                                                                                                     gggCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTagcagc                   >  2:215149/1‑140 (MQ=255)

                                                                                                                       gCTTCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCa                 <  1:948598/140‑1 (MQ=255)

                                                                                                                         ttCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCAtt               >  1:217111/1‑140 (MQ=255)

                                                                                                                         ttCATCATTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCAtt               >  1:1061998/1‑140 (MQ=255)

                                                                                                                           catcatTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTTACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTgg             >  1:550449/1‑140 (MQ=255)

                                                                                                                             tcatTTTTACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTg           >  2:1246640/1‑140 (MQ=255)

                                                                                                                                tttttACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACg        <  2:2137445/140‑1 (MQ=255)

                                                                                                                                tttttACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACg        <  1:1246640/140‑1 (MQ=255)

                                                                                                                                 ttttACCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGc       <  1:470081/140‑1 (MQ=255)

                                                                                                                                     aCCTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTccc   >  2:612642/1‑140 (MQ=255)

                                                                                                                                      ccTATTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCa  >  2:366587/1‑140 (MQ=255)

ATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCA  >  NC_000913/312652‑312925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A