Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F48 I1 R1 70 76.3 2572620 98.7% 2539175 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 693,687 G→T V423V (GTC→GTA)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913693,6870GT98.3% 209.7 / ‑2.8 60V423V (GTC→GTA) miaBisopentenyl‑adenosine A37 tRNA methylthiolase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (28/31);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGC  >  NC_000913/693550‑693823
                                                                                                                                         |                                                                                                                                        
aCGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctac                                                                                                                                        >  2:328375/1‑140 (MQ=255)
aCGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctac                                                                                                                                        >  2:718287/1‑140 (MQ=255)
aCGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctac                                                                                                                                        >  1:725606/1‑140 (MQ=255)
 cGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctacg                                                                                                                                       >  1:1037828/1‑140 (MQ=255)
 cGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctacg                                                                                                                                       >  1:920452/1‑140 (MQ=255)
 cGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctacg                                                                                                                                       >  1:211108/1‑140 (MQ=255)
  gCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATctacga                                                                                                                                      >  1:494159/1‑140 (MQ=255)
       ggTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTa                                                                                                                                 >  2:421752/1‑140 (MQ=255)
        gTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTAc                                                                                                                                <  2:165975/140‑1 (MQ=255)
        gTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTAc                                                                                                                                <  2:920452/140‑1 (MQ=255)
        gTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTAc                                                                                                                                <  2:1061870/140‑1 (MQ=255)
            ttttCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGAt                                                                                                                            >  2:934560/1‑140 (MQ=255)
            ttttCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGAt                                                                                                                            >  1:323019/1‑140 (MQ=255)
            ttttCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGAt                                                                                                                            >  1:386454/1‑140 (MQ=255)
                       ggTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCg                                                                                                                 <  2:548090/140‑1 (MQ=255)
                            gggCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCt                                                                                                            <  2:1233066/140‑1 (MQ=255)
                                 aTCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGaa                                                                                                       >  1:728282/1‑140 (MQ=255)
                                  tCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGaat                                                                                                      >  2:524278/1‑139 (MQ=255)
                                  tCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAg                                                                                                      >  1:366647/1‑140 (MQ=255)
                                   cACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGt                                                                                                     <  1:718287/140‑1 (MQ=255)
                                        aTTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGAcc                                                                                                >  2:1277648/1‑140 (MQ=255)
                                           ccGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcg                                                                                             <  2:144654/140‑1 (MQ=255)
                                           ccGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcg                                                                                             <  1:204975/140‑1 (MQ=255)
                                              gtgtTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCgg                                                                                          <  1:864323/140‑1 (MQ=255)
                                                gtTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGtt                                                                                        <  2:125602/140‑1 (MQ=255)
                                                    cTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTAttt                                                                                    >  1:658477/1‑140 (MQ=255)
                                                         aCGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGt                                                                               <  1:777014/140‑1 (MQ=255)
                                                          cgcgCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTa                                                                              <  2:494159/140‑1 (MQ=255)
                                                           gcgcAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTAc                                                                             >  1:272539/1‑140 (MQ=255)
                                                                 aCCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCgg                                                                       >  2:359789/1‑140 (MQ=255)
                                                                  cccATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGa                                                                      <  2:1037828/140‑1 (MQ=255)
                                                                     aTTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAg                                                                   <  1:1277648/140‑1 (MQ=255)
                                                                     aTTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAg                                                                   <  2:725606/140‑1 (MQ=255)
                                                                      tttCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGc                                                                  >  2:552343/1‑140 (MQ=255)
                                                                       ttCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCt                                                                 >  1:34427/1‑140 (MQ=255)
                                                                          aTCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCa                                                              <  2:950378/140‑1 (MQ=255)
                                                                           tCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCat                                                             >  1:372305/1‑140 (MQ=255)
                                                                             ttCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCatga                                                           <  1:328375/140‑1 (MQ=255)
                                                                             ttCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCatga                                                           >  1:332453/1‑140 (MQ=255)
                                                                                aGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGc                                                        >  2:23417/1‑140 (MQ=255)
                                                                                aGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGc                                                        >  2:622549/1‑140 (MQ=255)
                                                                                    cGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCtt                                                    <  1:1121899/140‑1 (MQ=255)
                                                                                     gCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTa                                                   <  1:1256388/140‑1 (MQ=255)
                                                                                       accacTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTAcg                                                 <  1:934560/140‑1 (MQ=255)
                                                                                            tttACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCTTGCCTTCGAAGTTGACCACGCGGTTATTTTCCTTACGCCCGGAATGTTCCATGATGTTCTTACGGGAtt                                            >  2:8852/1‑139 (MQ=255)
                                                                                            tttACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATg                                            <  1:622549/140‑1 (MQ=255)
                                                                                              tACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTa                                          <  1:421752/140‑1 (MQ=255)
                                                                                              tACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGATATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTa                                          >  2:127198/1‑140 (MQ=255)
                                                                                                ccGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTAcc                                        <  2:366647/140‑1 (MQ=255)
                                                                                                 cgcgCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCt                                       >  2:708986/1‑140 (MQ=255)
                                                                                                   cgcAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCTCGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCttt                                     >  2:969362/1‑139 (MQ=255)
                                                                                                   cgcAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGGAAGCTCCATGATGCTCTTACGCGATGTACCTTc                                     >  2:715008/1‑140 (MQ=255)
                                                                                                   cgcAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTc                                     >  2:1250463/1‑140 (MQ=255)
                                                                                                   cgcAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTc                                     >  2:1159866/1‑140 (MQ=255)
                                                                                                     cAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTa                                   <  1:442407/140‑1 (MQ=255)
                                                                                                     cAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTa                                   >  1:127761/1‑140 (MQ=255)
                                                                                                     cAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTa                                   >  1:847318/1‑140 (MQ=255)
                                                                                                      agagagTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGGAAGCTCCATGATGCTCTTACGCGATGTACCTTCTAc                                  <  1:715008/140‑1 (MQ=255)
                                                                                                         gagTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAg                               <  2:728282/140‑1 (MQ=255)
                                                                                                         gagTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTGCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCAACCAg                               <  1:127198/140‑1 (MQ=255)
                                                                                                          agTTCGGGTAGACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGa                              <  2:272539/140‑1 (MQ=255)
                                                                                                          agTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGa                              <  2:446821/140‑1 (MQ=255)
                                                                                                                    gACGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTggg                    <  1:1159866/140‑1 (MQ=255)
                                                                                                                      cGTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGggtg                  >  2:978831/1‑140 (MQ=255)
                                                                                                                       gTCGGTAATTTCTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGggtgg                 <  1:569179/140‑1 (MQ=255)
                                                                                                                                  cTACATCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATc      >  1:317263/1‑140 (MQ=255)
                                                                                                                                      aTCTACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCcggc  <  2:332453/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGC  >  NC_000913/693550‑693823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A