Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,923	A→G	intergenic (‑104/‑166)	ybjL ← / → ybjM	putative transport protein YbjL/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,923	0	A	G	100.0%	193.0 / NA	50	intergenic (‑104/‑166)	ybjL/ybjM	putative transport protein YbjL/putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/31); total (19/31)

CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAA  >  NC_000913/889788‑890053

ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                >  1:887047/1‑140 (MQ=255)

    ttAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagta                                                                                                                            >  1:694676/1‑140 (MQ=255)

     tAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaa                                                                                                                           <  1:430537/140‑1 (MQ=255)

          aTTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagtaa                                                                                                                      <  2:117965/140‑1 (MQ=255)

           ttCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAAc                                                                                                                     >  2:800196/1‑140 (MQ=255)

           ttCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAAc                                                                                                                     >  2:199012/1‑140 (MQ=255)

           ttCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGCAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAAc                                                                                                                     >  2:1264358/1‑140 (MQ=255)

            tCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACa                                                                                                                    <  2:457622/140‑1 (MQ=255)

            tCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACa                                                                                                                    <  1:700657/140‑1 (MQ=255)

             cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                   <  1:1073619/140‑1 (MQ=255)

             cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                   <  2:112257/140‑1 (MQ=255)

             cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                   <  1:308606/140‑1 (MQ=255)

                cGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatat                                                                                                                >  2:985924/1‑140 (MQ=255)

                     tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATAtttt                                                                                                           >  2:1002782/1‑140 (MQ=255)

                      ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                          <  2:227173/140‑1 (MQ=255)

                       tataTTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                         <  1:199012/140‑1 (MQ=255)

                       tataATCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAACGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                         <  1:444590/140‑1 (MQ=255)

                              aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGATAGAAATGGTAATTTACGATAATGGTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAgtgaa                                                                                                  >  1:925179/1‑138 (MQ=255)

                              aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                  <  2:5766/140‑1 (MQ=255)

                              aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                  <  2:694676/140‑1 (MQ=255)

                              aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                  >  1:428956/1‑140 (MQ=255)

                                ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                >  2:936140/1‑140 (MQ=255)

                                 gAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATc                                                                                               <  1:1271900/140‑1 (MQ=255)

                                    gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATcaca                                                                                            <  1:781040/140‑1 (MQ=255)

                                     cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACAt                                                                                           <  2:587922/140‑1 (MQ=255)

                                          ttcttGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGt                                                                                      <  1:1207534/140‑1 (MQ=255)

                                             ttGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATc                                                                                   <  2:513744/140‑1 (MQ=255)

                                               gTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCaa                                                                                 <  2:887047/140‑1 (MQ=255)

                                                          aGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCtatgaat                                                                      >  1:411726/1‑136 (MQ=255)

                                                          aGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGa                                                                      >  1:1140277/1‑140 (MQ=255)

                                                                tGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                <  2:427026/140‑1 (MQ=255)

                                                                tGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                <  1:936140/140‑1 (MQ=255)

                                                                   aagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTg                                                             <  2:1140277/140‑1 (MQ=255)

                                                                    agaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTgg                                                            <  1:1264358/140‑1 (MQ=255)

                                                                     gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                           <  1:985924/140‑1 (MQ=255)

                                                                      aaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                          <  1:120456/140‑1 (MQ=255)

                                                                      aaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                          >  2:870239/1‑140 (MQ=255)

                                                                             aaTTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTa                                                   >  2:880567/1‑140 (MQ=255)

                                                                                              tttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCg                                  <  2:38128/140‑1 (MQ=255)

                                                                                                 aCCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCt                               <  1:800196/140‑1 (MQ=255)

                                                                                                    aGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTa                            <  1:1199253/140‑1 (MQ=255)

                                                                                                           aGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACt                     <  2:260842/140‑1 (MQ=255)

                                                                                                             ggCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGa                   >  1:1057792/1‑140 (MQ=255)

                                                                                                             ggCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGa                   >  1:250000/1‑140 (MQ=255)

                                                                                                                     ttcattcaGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCcaca           <  2:479255/140‑1 (MQ=255)

                                                                                                                     ttcattcaGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCcaca           <  1:1002782/140‑1 (MQ=255)

                                                                                                                       cattcaGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGC‑‑TATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTgg       <  2:411726/140‑1 (MQ=255)

                                                                                                                            aGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGa    >  2:520449/1‑140 (MQ=255)

                                                                                                                             gcgcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaa   >  2:391616/1‑140 (MQ=255)

                                                                                                                              cgcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaa  >  2:309128/1‑140 (MQ=255)

CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAA  >  NC_000913/889788‑890053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A