Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F48 I1 R1 56 123.4 4244010 99.1% 4205813 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 205,699 C→A R192S (CGC→AGC)  dnaE → DNA polymerase III subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913205,6990CA100.0% 431.1 / NA 122R192S (CGC→AGC) dnaEDNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (71/51);  total (71/51)

Only 100 of 131 total aligned reads displayed.

GCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGC  >  NC_000913/205568‑205834
                                                                                                                                   |                                                                                                                                       
gcgcgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTg                                                                                                                                 >  2:1561246/1‑140 (MQ=255)
 cgcgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGc                                                                                                                                >  1:181900/1‑140 (MQ=255)
   cgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGccc                                                                                                                              >  1:1754277/1‑140 (MQ=255)
   cgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGccc                                                                                                                              >  2:1113922/1‑140 (MQ=255)
   cgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGccc                                                                                                                              >  2:883788/1‑140 (MQ=255)
   cgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGccc                                                                                                                              >  2:1253439/1‑140 (MQ=255)
   cgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGccc                                                                                                                              >  1:1150072/1‑140 (MQ=255)
   cgcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGccc                                                                                                                              <  2:1622468/140‑1 (MQ=255)
    gcTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCcg                                                                                                                             >  2:2062268/1‑140 (MQ=255)
                gtgtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCa                                                                                                                 >  1:707006/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGATCTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  1:1090975/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  1:780430/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  1:498116/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  2:458132/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  1:331229/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  2:383944/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  2:1841271/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  1:1073156/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  2:302858/1‑140 (MQ=255)
                  gtgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAac                                                                                                               >  2:1142569/1‑140 (MQ=255)
                   tgtCGCGTTTTATGAAGAACACTTCCTGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAacg                                                                                                              >  1:1085490/1‑140 (MQ=255)
                   tgtCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAacg                                                                                                              >  1:131496/1‑140 (MQ=255)
                     tCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACAGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAacgac                                                                                                            >  1:1184433/1‑140 (MQ=255)
                      cgcgTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAacgacg                                                                                                           >  1:2007102/1‑140 (MQ=255)
                       gcgTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGt                                                                                                          <  2:181900/140‑1 (MQ=255)
                         gTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACTGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTgc                                                                                                        <  2:1974179/140‑1 (MQ=255)
                         gTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTgc                                                                                                        <  2:1445943/140‑1 (MQ=255)
                             tATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCtt                                                                                                    >  1:1375575/1‑140 (MQ=255)
                               tGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTa                                                                                                  >  2:1172946/1‑140 (MQ=255)
                                  agaaCACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCg                                                                                               <  2:2007102/140‑1 (MQ=255)
                                    aaCACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGAc                                                                                             >  1:369167/1‑140 (MQ=255)
                                    aaCACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGAc                                                                                             <  1:302858/140‑1 (MQ=255)
                                     acacTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGAca                                                                                            <  2:1972369/140‑1 (MQ=255)
                                         ttCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCt                                                                                                     <  1:1924533/127‑1 (MQ=255)
                                         ttCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCt                                                                                                     >  2:1924533/1‑127 (MQ=255)
                                         ttCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGAcagcag                                                                                        >  1:972163/1‑140 (MQ=255)
                                               gATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACtt                                                                                  <  1:1253439/140‑1 (MQ=255)
                                                    cTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACg                                                                             <  1:1095250/140‑1 (MQ=255)
                                                     tATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGc                                                                            >  1:1594493/1‑140 (MQ=255)
                                                      aTTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGca                                                                           <  2:579637/140‑1 (MQ=255)
                                                              gAGCTGATCCGCACCGGCAGGCCGGATGTAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAAt                                                                   <  2:235151/140‑1 (MQ=255)
                                                               aGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATc                                                                  >  1:1177585/1‑140 (MQ=255)
                                                               aGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATc                                                                  >  1:2038500/1‑140 (MQ=255)
                                                               aGCTAATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGATTGCCCGTTGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATc                                                                  >  2:1848226/1‑140 (MQ=255)
                                                                    aTCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGt                                                             >  1:441666/1‑140 (MQ=255)
                                                                    aTCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGt                                                             >  1:697240/1‑140 (MQ=255)
                                                                    aTCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGt                                                             >  1:1271224/1‑140 (MQ=255)
                                                                    aTCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGt                                                             >  1:1741612/1‑140 (MQ=255)
                                                                      ccGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTcg                                                           <  1:458132/140‑1 (MQ=255)
                                                                      ccGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTcg                                                           <  1:883788/140‑1 (MQ=255)
                                                                      ccGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTcg                                                           <  2:1085490/140‑1 (MQ=255)
                                                                           ccGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATc                                                      <  2:697240/140‑1 (MQ=255)
                                                                            cGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATcc                                                     >  1:1209214/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTcg                                                           <  2:1365452/132‑1 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  1:924891/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  2:624026/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  2:328906/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  2:2106275/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  2:1138499/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  1:1907014/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCac                                                   >  1:1600327/1‑140 (MQ=255)
                                                                              gCAGGCCGGATGAAGAAAGCAATCTGCACGCGGCGGTGGAACTGGCGGAAGCGGGCGGCATGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGTGACTTTGACGCACACGAACTCCGCGTCCCGATCCat                                                   >  2:637993/1‑139 (MQ=255)
                                                                               cAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCacg                                                  <  1:2106275/140‑1 (MQ=255)
                                                                                aGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCacga                                                 <  1:1990056/140‑1 (MQ=255)
                                                                                aGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCacga                                                 <  2:1096108/140‑1 (MQ=255)
                                                                                aGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCacga                                                 <  2:1687412/140‑1 (MQ=255)
                                                                                     ggATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCt                                            <  1:985075/140‑1 (MQ=255)
                                                                                     ggATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCt                                            <  1:2119416/140‑1 (MQ=255)
                                                                                            gaaAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCt                                     <  1:1138499/140‑1 (MQ=255)
                                                                                               aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcga                                  <  2:621206/140‑1 (MQ=255)
                                                                                               aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcga                                  <  1:1971033/140‑1 (MQ=255)
                                                                                               aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcga                                  <  2:1782533/140‑1 (MQ=255)
                                                                                               aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcga                                  <  2:1714972/140‑1 (MQ=255)
                                                                                               aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcga                                  <  1:905513/140‑1 (MQ=255)
                                                                                                gCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcgac                                 <  1:1488143/140‑1 (MQ=255)
                                                                                                 cTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcgacg                                >  1:793587/1‑140 (MQ=255)
                                                                                                 cTATCTGCACGCGGCGGGGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTcgacg                                >  1:963204/1‑140 (MQ=255)
                                                                                                    tCTGCACGCGGCGGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATc                             >  2:2117488/1‑140 (MQ=255)
                                                                                                           gcggcgGTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACg                      <  1:1894812/140‑1 (MQ=255)
                                                                                                            cggcggTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGc                     <  1:1172946/140‑1 (MQ=255)
                                                                                                              gcggTGGAACTGGCGGAAGCGAGTGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGccc                   >  1:1677675/1‑140 (MQ=255)
                                                                                                              gcggTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGccc                   >  2:853933/1‑140 (MQ=255)
                                                                                                              gcggTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGccc                   >  1:1164539/1‑140 (MQ=255)
                                                                                                               cggTGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCcg                  <  2:1594493/140‑1 (MQ=255)
                                                                                                                  tGGAACTGGCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGt               >  1:1124110/1‑140 (MQ=255)
                                                                                                                         ggCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGc                     <  2:647130/127‑1 (MQ=255)
                                                                                                                         ggCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGc                     >  1:647130/1‑127 (MQ=255)
                                                                                                                         ggCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTAtt        >  1:1281430/1‑140 (MQ=255)
                                                                                                                          gCGGAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTc       >  1:1374611/1‑140 (MQ=255)
                                                                                                                            ggAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgc     <  2:966089/140‑1 (MQ=255)
                                                                                                                            ggAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgc     <  1:1264475/140‑1 (MQ=255)
                                                                                                                            ggAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgc     <  2:441666/140‑1 (MQ=255)
                                                                                                                            ggAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgc     <  2:1375575/140‑1 (MQ=255)
                                                                                                                            ggAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgc     >  2:1160837/1‑140 (MQ=255)
                                                                                                                             gAAGCGAGCGGTTTGCCCGTCGTGGCGGCCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgcc    <  2:131496/140‑1 (MQ=255)
                                                                                                                             gAAGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgcc    <  2:707006/140‑1 (MQ=255)
                                                                                                                              aaGCGAGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccg   <  1:624026/140‑1 (MQ=255)
                                                                                                                               agcgagcgGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACTGCAGCGACTTTGACGCACAAGAAATCAGCGTCGCGATTCACGACGGCTTTACCCTCGACGATCCTAAACACCCGCGTAACTACTcgccgt  <  2:1998653/140‑2 (MQ=255)
                                                                                                                               agcgagcgGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccgc  <  2:331229/140‑1 (MQ=255)
                                                                                                                               agcgagcgGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccgc  <  1:2117488/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTATTTTCTCGAGCTGATCCGCACCGGCAGGCCGGATGAAGAAAGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGC  >  NC_000913/205568‑205834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A