Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F48 I1 R1	56	123.4	4244010	99.1%	4205813	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,409	G→T	intergenic (‑76/‑184)	yaiY ← / → yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,409	0	G	T	100.0%	367.9 / NA	103	intergenic (‑76/‑184)	yaiY/yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (44/59); total (44/59)

Only 100 of 103 total aligned reads displayed.

TTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGAC  >  NC_000913/399273‑399543

ttGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATAtt                                                                                                                                     >  1:466172/1‑140 (MQ=255)

  gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGTAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGt                                                                                                                                   >  2:196702/1‑140 (MQ=255)

  gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGt                                                                                                                                   <  1:1845587/140‑1 (MQ=255)

  gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGt                                                                                                                                   >  2:1151779/1‑140 (MQ=255)

  gAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGt                                                                                                                                   >  1:1532912/1‑140 (MQ=255)

   aaGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTc                                                                                                                                  <  1:855490/140‑1 (MQ=255)

    agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCa                                                                                                                                 <  1:276154/140‑1 (MQ=255)

    agagGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCa                                                                                                                                 <  2:442029/140‑1 (MQ=255)

        gCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAatat                                                                                                                             >  2:1281737/1‑140 (MQ=255)

        gCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAatat                                                                                                                             >  2:1743112/1‑140 (MQ=255)

        gCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAatat                                                                                                                             <  2:2036222/140‑1 (MQ=255)

        gCATTGCGATATTTTCCGCGAAAAAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAatat                                                                                                                             <  2:1667128/140‑1 (MQ=255)

           ttGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAg                                                                                                                          <  1:685303/140‑1 (MQ=255)

           ttGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAg                                                                                                                          >  2:1535748/1‑140 (MQ=255)

             gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGaa                                                                                                                        >  1:1682090/1‑140 (MQ=255)

             gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGaa                                                                                                                        <  2:220692/140‑1 (MQ=255)

             gCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGaa                                                                                                                        <  2:1525724/140‑1 (MQ=255)

             gCGATATTTTCCGCTAAACAGCGATTTTGACA‑GGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAAt                                                                                                                       >  2:651843/1‑140 (MQ=255)

              cGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATGTCAGAAt                                                                                                                       <  1:1273421/140‑1 (MQ=255)

              cGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAAt                                                                                                                       <  1:1541228/140‑1 (MQ=255)

               gATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATc                                                                                                                      <  2:152125/140‑1 (MQ=255)

                atatTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCa                                                                                                                     <  2:1604708/140‑1 (MQ=255)

                  atTTTCCGTTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATg                                                                                                                   <  1:1929862/140‑1 (MQ=255)

                   ttttCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATgg                                                                                                                  >  1:1466826/1‑140 (MQ=255)

                   ttttCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATgg                                                                                                                  <  2:600814/140‑1 (MQ=255)

                   ttttCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATgg                                                                                                                  <  2:1532912/140‑1 (MQ=255)

                      tCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTaa                                                                                                               >  1:6740/1‑140 (MQ=255)

                      tCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTaa                                                                                                               >  1:641279/1‑140 (MQ=255)

                      tCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTaa                                                                                                               >  2:21053/1‑140 (MQ=255)

                      tCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTaa                                                                                                               >  1:82374/1‑140 (MQ=255)

                        cGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAAt                                                                                                             >  2:2098752/1‑140 (MQ=255)

                        cGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAAt                                                                                                             <  2:1335303/140‑1 (MQ=255)

                        cGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAAt                                                                                                             >  2:566458/1‑140 (MQ=255)

                          cTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTg                                                                                                           >  1:881920/1‑140 (MQ=255)

                            aaaCAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGAt                                                                                                         <  1:175476/140‑1 (MQ=255)

                             aaCAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATg                                                                                                        >  1:1047756/1‑140 (MQ=255)

                             aaCAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATg                                                                                                        >  2:1320359/1‑140 (MQ=255)

                               cAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGtt                                                                                                      <  2:1030905/140‑1 (MQ=255)

                               aaGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGtt                                                                                                      <  2:1115105/139‑1 (MQ=255)

                                 gCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTgg                                                                                                    <  2:1466826/140‑1 (MQ=255)

                                 gCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTgg                                                                                                    <  2:6740/140‑1 (MQ=255)

                                    aTTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGaa                                                                                                 <  2:1298802/140‑1 (MQ=255)

                                    aTTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGaa                                                                                                 <  2:346476/140‑1 (MQ=255)

                                    aTTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGaa                                                                                                 <  2:868547/140‑1 (MQ=255)

                                            aGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCg                                                                                         <  1:830094/140‑1 (MQ=255)

                                               gTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATA‑CAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGc                                                                                     >  1:306264/1‑140 (MQ=255)

                                                  aaaTCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATA‑CAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTg                                                                                  <  2:306264/140‑1 (MQ=255)

                                                        gCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCt                                                                             >  1:714272/1‑140 (MQ=255)

                                                        gCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCt                                                                             >  2:484548/1‑140 (MQ=255)

                                                         ccATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCtt                                                                            >  2:1179646/1‑140 (MQ=255)

                                                         ccATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCtt                                                                            >  2:235573/1‑140 (MQ=255)

                                                               aaaCCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTtgt                                                                      <  1:1281737/140‑1 (MQ=255)

                                                                   cTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGt                                                                  <  1:2098752/140‑1 (MQ=255)

                                                                    tCCGGAAGTCATCTGCTTCTCTTCTGGGGTGATACCAGCGGCTTATGGCAAGGCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTc                                                                 >  1:2004204/1‑140 (MQ=255)

                                                                             cATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATgg                                                        <  1:242505/140‑1 (MQ=255)

                                                                                  gCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAAcc                                                   <  1:1703424/140‑1 (MQ=255)

                                                                                  gCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAAcc                                                   <  1:1100449/140‑1 (MQ=255)

                                                                                  gCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAAcc                                                   <  1:1695999/140‑1 (MQ=255)

                                                                                     tctcttctGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTa                                                >  1:945699/1‑140 (MQ=255)

                                                                                        cttctGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGt                                             >  2:2012946/1‑140 (MQ=255)

                                                                                        cttctGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGt                                             >  2:688939/1‑140 (MQ=255)

                                                                                          tctGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTa                                           <  2:1409286/140‑1 (MQ=255)

                                                                                          tctGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTa                                           <  2:1145085/140‑1 (MQ=255)

                                                                                           ctGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTaa                                          >  2:172196/1‑140 (MQ=255)

                                                                                             ggTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAAt                                        >  2:202483/1‑140 (MQ=255)

                                                                                                gtgATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATtta                                     >  2:2053921/1‑140 (MQ=255)

                                                                                                   aTACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATttatta                                  >  2:1459518/1‑140 (MQ=255)

                                                                                                   aTACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATttatta                                  >  2:1252576/1‑140 (MQ=255)

                                                                                                   aTACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATttatta                                  >  1:1231276/1‑140 (MQ=255)

                                                                                                   aTACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAAATGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATttatta                                  <  1:1005614/140‑1 (MQ=255)

                                                                                                     aCCAGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAAc                                <  1:2053921/140‑1 (MQ=255)

                                                                                                      ccAGCGGCTTATGGCAAGTCTGACAGGTTATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACa                               <  2:1995545/140‑1 (MQ=255)

                                                                                                        aGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAAt                             <  1:952559/140‑1 (MQ=255)

                                                                                                        aGCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAAt                             >  1:497966/1‑140 (MQ=255)

                                                                                                         gCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAAtt                            >  2:1247675/1‑140 (MQ=255)

                                                                                                         gCGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAAtt                            >  2:357299/1‑140 (MQ=255)

                                                                                                          cGGCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTa                           >  2:855301/1‑140 (MQ=255)

                                                                                                           ggCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTAc                          >  2:2094682/1‑140 (MQ=255)

                                                                                                            gCTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACg                         <  2:227442/140‑1 (MQ=255)

                                                                                                             cTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGa                        <  1:357299/140‑1 (MQ=255)

                                                                                                             cTTATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGa                        >  2:515763/1‑140 (MQ=255)

                                                                                                               tATGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGaaa                      <  2:1231276/140‑1 (MQ=255)

                                                                                                                 tGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAt                    <  1:196702/140‑1 (MQ=255)

                                                                                                                 tGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAt                    <  1:1252576/140‑1 (MQ=255)

                                                                                                                 tGGCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATATTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAt                    <  1:1719666/140‑1 (MQ=255)

                                                                                                                  ggCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAtg                   <  1:1459518/140‑1 (MQ=255)

                                                                                                                  ggCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAtg                   <  2:641279/140‑1 (MQ=255)

                                                                                                                  ggCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAtg                   <  1:1084515/140‑1 (MQ=255)

                                                                                                                  ggCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAtg                   <  2:80566/140‑1 (MQ=255)

                                                                                                                  ggCAAGTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAAtg                   <  2:82374/140‑1 (MQ=255)

                                                                                                                       gTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTc              >  1:363345/1‑140 (MQ=255)

                                                                                                                       gTCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTc              <  2:1074440/140‑1 (MQ=255)

                                                                                                                       gTCTGACAGGTCATTTATAGTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTc              <  1:305744/140‑1 (MQ=255)

                                                                                                                        tCTGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCAGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCt             <  1:1419263/140‑1 (MQ=255)

                                                                                                                          tGACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTac           <  1:984695/140‑1 (MQ=255)

                                                                                                                           gACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTaca          <  2:484912/140‑1 (MQ=255)

                                                                                                                           gACAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTaca          <  1:1743112/140‑1 (MQ=255)

                                                                                                                            aCAGGTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTacac         >  2:1057585/1‑140 (MQ=255)

                                                                                                                                gTCATTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACcaca     <  1:484548/140‑1 (MQ=255)

                                                                                                                                   aTTTATATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGAc  <  1:1105110/140‑1 (MQ=255)

TTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATTGTCAATATCAGAATCATGGTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGAC  >  NC_000913/399273‑399543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A