Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F48 I1 R1
|
56 |
123.4 |
4244010 |
99.1% |
4205813 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
823,340 |
C→A |
E134* (GAG→TAG) |
clsB ← |
cardiolipin synthase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 823,340 | 0 | C | A | 100.0%
| 98.0
/ NA
| 30 | E134* (GAG→TAG) | clsB | cardiolipin synthase B |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/3); total (27/3) |
GCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGC > NC_000913/823208‑823474
|
gCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAg > 2:881388/1‑140 (MQ=255)
cgtcgtGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑ATAGCTGGACATATGCTAGGCGGAGTAATTCAg > 2:2065672/1‑140 (MQ=255)
gCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGAAATATGCTAGGCGGAGTAATTCAGCCCGcc > 2:2067441/1‑140 (MQ=255)
tgcTCTGTGCAGGCCGTTTTCCCACCTCAAAATGGAAATTATCTTCGACAGTCGGCCCTTAATGGCGTCCCGGGTAATCCTTTTTA‑GCCTCT‑GAACC‑TTAGCTGGACATGTGTTAGGCGGTTTAATTCAGCCCGCCAATa < 2:91305/139‑1 (MQ=255)
cgcTCTGTCAAGGCAGGTTTTCCAGCAAAAACGGAAGAATATCTCCGCCAATCGGCCTTTAAGGGCGTCCCGCGTAACCTTGTTTG‑GCCTTT‑GGCCC‑GTAGCTGGCCATATGCTGGGGGGAGTAATTCAGCCCGCCAATa < 2:1363345/140‑1 (MQ=255)
tGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGc > 1:2000171/1‑140 (MQ=255)
ttttCCAGCTCAAACTGGAGAATATCTTCGGCAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGTCC‑GAAGCTGGACATATGCTAGGCGGAGCAATTCAGCCCGCCAATATATGCTATACGCGCGTc > 2:578047/1‑140 (MQ=255)
ttttCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTc > 2:630346/1‑140 (MQ=255)
ttttCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTc > 2:2120317/1‑140 (MQ=255)
ttttCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTc > 2:1190783/1‑140 (MQ=255)
ttttCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTc > 1:1716357/1‑140 (MQ=255)
ttttCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGGAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTc > 1:1840334/1‑140 (MQ=255)
ccAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATc > 1:820983/1‑140 (MQ=255)
agaATATCTTCGACAACCGCCCCTTCAAGGCGTACCGCGTAATCTTG‑TTA‑ACCTCTGGGACG‑GAACCTATACATATTCAGGGCTGCTTATCTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCCCCACAATTTTGcc < 2:2000171/140‑2 (MQ=255)
atatCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATg > 2:1764037/1‑140 (MQ=255)
cTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCTTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATc > 1:280553/1‑140 (MQ=255)
cTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATc > 2:129529/1‑140 (MQ=255)
cTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATc > 1:1546081/1‑140 (MQ=255)
tCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCg > 2:981076/1‑140 (MQ=255)
caaCAATCGGCCCTCAAAGGCGTCCCGCGAAATCCTGTTTA‑GCCCCT‑GGACC‑GTAGCTGGACATATGCTGGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCgg < 2:151806/138‑1 (MQ=255)
aCAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCg < 1:881388/140‑1 (MQ=255)
aCAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCg > 1:579224/1‑140 (MQ=255)
aCAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCg > 1:365386/1‑140 (MQ=255)
aCAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCg > 2:382661/1‑140 (MQ=255)
aCAATCGGCCCTTAAAGGCGTCCCGCGTAATCTTGTTTA‑GCCTCT‑GGACC‑GTAGCTGAACATATGCTGGGGGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCg < 2:1460707/140‑1 (MQ=255)
tCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGCTGCATCCGGCGAAac > 2:1304937/1‑140 (MQ=255)
gCCCTTAAAGGCGCGCCGCGTAATCCTTTTTA‑GCCTC‑‑GGACCTGTAGGGGGACATATGCTAGGCGGAGTATGTCACCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAAcaca < 2:1840334/140‑1 (MQ=255)
cgcgTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCaa > 2:2029052/1‑140 (MQ=255)
cgTAAATCTTTTTAGGCCTCG‑GGACC‑TTAGCTGGATTTATGCTAGGGGGAGAAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCaaa < 2:579224/140‑1 (MQ=255)
tAATCCTGTTTA‑GCCTCT‑GGTCC‑GTAGCTGGACATATGCTAGGCGGAGTATTTCAGCCCGCCAATAAAGGCTATACGCGCGTCTATCACTACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAg > 2:1251674/1‑140 (MQ=255)
acccTGTTTA‑GCCTCT‑GGACC‑GTAGCTGAACATATGCTAGGGGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGc < 1:1957910/138‑1 (MQ=255)
aTCCTGTTTA‑GCTCCT‑GGCCC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGc < 2:732347/140‑1 (MQ=255)
tcttttttA‑GCCTCG‑GGACC‑GTGGCGGGACATATGCTGGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCg < 2:1549202/135‑1 (MQ=255)
ttA‑GCCTCT‑GGCCG‑GTAGCTGGACATATGCTAGGGGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCg < 1:310388/140‑1 (MQ=255)
ttA‑GCCTCT‑GGCCC‑GTAGCTGGACATATGCTGGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCg < 1:305451/140‑1 (MQ=255)
ttA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCg > 2:88535/1‑140 (MQ=255)
ttA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCg < 1:981076/140‑1 (MQ=255)
ccTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGa > 1:456834/1‑140 (MQ=255)
ccTCT‑GGACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGa > 1:1017606/1‑140 (MQ=255)
ccTCT‑GGACC‑GTAGCTGGACATATGCCAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACTAAAAAGGCGAGGGCGGGGa > 1:1482808/1‑140 (MQ=255)
ctct‑GGACC‑ATAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGAt < 1:2065672/140‑1 (MQ=255)
gggACC‑GTAGCTGGACATATGCTAGGGGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgt < 1:126281/139‑1 (MQ=255)
ggACC‑GTAGCTGGACATATGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgta > 1:96685/1‑140 (MQ=255)
gACC‑GTAGCTGGACATATGCTAGGGGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgtag < 1:443498/140‑1 (MQ=255)
caCC‑GTAGGTGAACATATGAGGGGGGGTGTAATCCGGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgtag < 1:1071915/139‑1 (MQ=255)
agtagaaCATATCATGGGGGGAGTAATTCGGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCgg < 2:1739172/134‑1 (MQ=255)
gtagACAAAGGCTGGGGGAAGAAATTAAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACC‑AAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac < 2:820983/137‑1 (MQ=255)
gggAAATATGCTGGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac < 1:665079/139‑1 (MQ=255)
atGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGc > 2:1909859/1‑140 (MQ=255)
atGCTAGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGc > 2:1613563/1‑140 (MQ=255)
|
GCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTA‑GCCTCT‑GGACC‑GTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGC > NC_000913/823208‑823474
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
