Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	409,944	G→T	Q26K (CAG→AAG)	rdgC ←	nucleoid‑associated protein RdgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	409,944	0	G	T	100.0%	113.5 / NA	33	Q26K (CAG→AAG)	rdgC	nucleoid‑associated protein RdgC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/17); total (16/17)

CTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGG  >  NC_000913/409809‑410077

cttTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCtttttt                                                                                                                                   <  2:845706/140‑1 (MQ=255)

               tAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCa                                                                                                                    <  1:834794/140‑1 (MQ=255)

                aaCAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCAc                                                                                                                   <  2:26028/140‑1 (MQ=255)

                aaCAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCAc                                                                                                                   <  1:127146/140‑1 (MQ=255)

                 aCAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACg                                                                                                                  <  1:613188/140‑1 (MQ=255)

                   aaTTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCa                                                                                                                <  1:245310/140‑1 (MQ=255)

                        gACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACg                                                                                                                  <  2:364730/133‑1 (MQ=255)

                        gACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACg                                                                                                                  >  1:364730/1‑133 (MQ=255)

                        gACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGaa                                                                                                           >  1:199006/1‑140 (MQ=255)

                        gACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGaa                                                                                                           >  1:230279/1‑140 (MQ=255)

                        gACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGaa                                                                                                           >  1:458578/1‑140 (MQ=255)

                              tGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTcg                                                                                                     >  2:697560/1‑140 (MQ=255)

                               ggCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGACATCTcgc                                                                                                    >  2:5078/1‑140 (MQ=255)

                                     gTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTaa                                                                                              <  2:513331/140‑1 (MQ=255)

                                        cGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGAc                                                                                           >  2:189600/1‑140 (MQ=255)

                                                    gCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTa                                                                               <  1:387732/140‑1 (MQ=255)

                                                                 aTCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACca                                                                  <  2:639656/140‑1 (MQ=255)

                                                                            ccAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATaa                                                       <  1:144106/140‑1 (MQ=255)

                                                                                  cATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCa                                                 <  2:837505/140‑1 (MQ=255)

                                                                                   aTCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCAc                                                <  1:5078/140‑1 (MQ=255)

                                                                                    tCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACg                                               <  2:199006/140‑1 (MQ=255)

                                                                                                         cGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                          >  1:740482/1‑140 (MQ=255)

                                                                                                         cGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                          >  2:754468/1‑140 (MQ=255)

                                                                                                         cGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCg                          >  1:53663/1‑140 (MQ=255)

                                                                                                         cGCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGCAAACCATTAAATTTTTGACCCACATCATGTTAATTTCCACGGCCTTGTCGTTAAATTTatct                          >  2:15599/1‑137 (MQ=255)

                                                                                                          gCATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCgg                         <  1:422162/140‑1 (MQ=255)

                                                                                                           cATGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCggg                        <  1:183452/140‑1 (MQ=255)

                                                                                                             tGGGGTAAATGCCATCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                      <  2:458578/140‑1 (MQ=255)

                                                                                                                          aTCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                      >  2:165746/1‑127 (MQ=255)

                                                                                                                          aTCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCa                      <  1:165746/127‑1 (MQ=255)

                                                                                                                          aTCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAAttt         >  2:312184/1‑139 (MQ=255)

                                                                                                                           tCGAGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGt        >  2:384841/1‑140 (MQ=255)

                                                                                                                              aGGCTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcgg     >  1:573333/1‑140 (MQ=255)

                                                                                                                                 cTAGCTTTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTcggcgg  >  1:625333/1‑140 (MQ=255)

CTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAATGCCATCGAGGCTAGCTGTTTTTCCATCTCTTCTGCACGCAGCGAAATCTCGCGGCTAAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCCTTGTCGTTAAATTTAGCGGGCATGATAACGAATTGTCGGCGG  >  NC_000913/409809‑410077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A