Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	743,216	G→T	W130C (TGG→TGT)	ybgI →	radiation resistance protein YbgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	743,216	0	G	T	100.0%	105.4 / NA	32	W130C (TGG→TGT)	ybgI	radiation resistance protein YbgI
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/13); total (19/13)

ATATCAACCTGTATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGGGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGG  >  NC_000913/743095‑743347

atatCAACCTGTATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCt                                                                                                                   <  2:192007/140‑1 (MQ=255)

          gTATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGAc                                                                                                         <  2:735238/140‑1 (MQ=255)

           tATGGCTGGCATTTGCCGCTTGACGCACATCTTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACt                                                                                                        >  1:618439/1‑140 (MQ=255)

           tATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACt                                                                                                        >  2:656157/1‑140 (MQ=255)

           tATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACt                                                                                                        >  2:43854/1‑140 (MQ=255)

           tATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACt                                                                                                        >  2:512001/1‑140 (MQ=255)

                 tggcATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCt                                                                                                  >  1:578005/1‑140 (MQ=255)

                  ggcATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTg                                                                                                 <  1:618184/140‑1 (MQ=255)

                  ggcATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTg                                                                                                 >  1:99697/1‑140 (MQ=255)

                     aTTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCt                                                                                              >  2:83676/1‑140 (MQ=255)

                         gCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCt                                                                                          >  1:293479/1‑140 (MQ=255)

                                gACGCACATCCTGAGCT‑GGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGACATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGaa                                                                                  >  2:380208/1‑140 (MQ=255)

                                               cTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACg                                                                    >  1:340191/1‑140 (MQ=255)

                                                 gggCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTa                                                                  <  2:483616/140‑1 (MQ=255)

                                                 gggCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTa                                                                  >  1:330425/1‑140 (MQ=255)

                                                  ggCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTaa                                                                 >  1:97853/1‑140 (MQ=255)

                                                   gCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAg                                                                <  2:97853/140‑1 (MQ=255)

                                                   gCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAg                                                                <  2:618439/140‑1 (MQ=255)

                                                   gCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAg                                                                <  1:43854/140‑1 (MQ=255)

                                                      aTAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCg                                                             <  2:293479/140‑1 (MQ=255)

                                                       aagcGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGt                                                            <  2:800388/137‑1 (MQ=255)

                                                            cacaGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATgt                                                       >  2:50238/1‑139 (MQ=255)

                                                              aaGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGgtg                                                     <  2:69300/139‑1 (MQ=255)

                                                                   ggcggcGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCg                                                >  1:208998/1‑140 (MQ=255)

                                                                     cggcgTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGAt                                              >  2:182266/1‑140 (MQ=255)

                                                                             atGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGAcc                                      <  2:331320/139‑1 (MQ=255)

                                                                                 ggATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAg                                  <  2:208998/140‑1 (MQ=255)

                                                                                     cACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCTGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAggtgg                              >  2:488280/1‑140 (MQ=255)

                                                                                               ggCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGt                    >  2:404345/1‑140 (MQ=255)

                                                                                               ggCGAAATTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGt                    >  1:430410/1‑140 (MQ=255)

                                                                                                   aaaTTGAGCCGTTGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGcc                <  2:262705/140‑1 (MQ=255)

                                                                                                              ttGGTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACggg     >  1:182688/1‑140 (MQ=255)

                                                                                                                 gTGCCGTGTGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGATTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGggcgg  <  1:380208/140‑1 (MQ=255)

ATATCAACCTGTATGGCTGGCATTTGCCGCTTGACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTCATGGGCGAAATTGAGCCGTTGGTGCCGTGGGGCGAACTGACCATGCCTGTGCCGGGACTGGAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGG  >  NC_000913/743095‑743347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A