Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	904,732	C→A	S47Y (TCC→TAC)	ybjQ →	putative heavy metal binding protein YbjQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	904,732	0	C	A	100.0%	140.3 / NA	40	S47Y (TCC→TAC)	ybjQ	putative heavy metal binding protein YbjQ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/22); total (18/22)

CAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACGGCAG  >  NC_000913/904596‑904867

cAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACgg                                                                                                                                      >  1:614153/1‑140 (MQ=255)

      tCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTa                                                                                                                                <  1:284033/140‑1 (MQ=255)

      tCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTa                                                                                                                                <  1:433395/140‑1 (MQ=255)

             ccccAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGaaaaa                                                                                                                         >  2:796192/1‑140 (MQ=255)

                cAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGaa                                                                                                                      <  2:14574/140‑1 (MQ=255)

                cAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGaa                                                                                                                      <  2:792369/140‑1 (MQ=255)

                cAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGaa                                                                                                                      <  1:474858/140‑1 (MQ=255)

                     ctGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCg                                                                                                                 <  2:578230/140‑1 (MQ=255)

                     ctGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCg                                                                                                                 >  1:724168/1‑140 (MQ=255)

                       ggAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTa                                                                                                               <  1:796192/140‑1 (MQ=255)

                        gAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTaa                                                                                                              >  1:611246/1‑140 (MQ=255)

                             ccAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCAc                                                                                                         <  2:437390/140‑1 (MQ=255)

                             ccAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCAc                                                                                                         <  1:571046/140‑1 (MQ=255)

                                            atatTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTg                                                                                          >  1:539275/1‑140 (MQ=255)

                                            atatTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTg                                                                                          <  2:45035/140‑1 (MQ=255)

                                                   ggTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAAtt                                                                                   <  2:22804/140‑1 (MQ=255)

                                                     tgtgGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAg                                                                                 >  1:535810/1‑140 (MQ=255)

                                                            aCCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCa                                                                          >  1:779959/1‑140 (MQ=255)

                                                            aCCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCa                                                                          >  2:584090/1‑140 (MQ=255)

                                                               ggCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGgc                                                                       >  1:846656/1‑140 (MQ=255)

                                                                gCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGgcg                                                                      <  2:295390/140‑1 (MQ=255)

                                                                         ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTg                                                             <  2:846656/140‑1 (MQ=255)

                                                                                   cAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACTTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATg                                                   >  2:209448/1‑140 (MQ=255)

                                                                                   cAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATg                                                   >  1:15481/1‑140 (MQ=255)

                                                                                   cAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATg                                                   >  2:457174/1‑140 (MQ=255)

                                                                                   cAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATg                                                   >  1:644290/1‑140 (MQ=255)

                                                                                   cAATATTTTCCG‑GATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGc                                                  >  2:175004/1‑140 (MQ=255)

                                                                                          ttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCcgtcgt                                            <  2:292116/140‑1 (MQ=255)

                                                                                          ttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCcgtcgt                                            <  2:12331/140‑1 (MQ=255)

                                                                                          ttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCcgtcgt                                            <  2:539275/140‑1 (MQ=255)

                                                                                          ttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCcgtcgt                                            <  1:584090/140‑1 (MQ=255)

                                                                                           tCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCgtcgtc                                           <  1:830857/140‑1 (MQ=255)

                                                                                             cGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGtcgtcgt                                         >  2:92606/1‑139 (MQ=255)

                                                                                                         gCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAGCTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGa                             <  2:759581/140‑1 (MQ=255)

                                                                                                                          cGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGc            >  2:381231/1‑140 (MQ=255)

                                                                                                                          cGTTGGCGGACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGc            >  1:492139/1‑140 (MQ=255)

                                                                                                                                 ggACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACgg     <  2:163316/140‑1 (MQ=255)

                                                                                                                                 ggACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACgg     <  1:457174/140‑1 (MQ=255)

                                                                                                                                 ggACGTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACgg     <  2:611246/140‑1 (MQ=255)

                                                                                                                                    cGTTACGGTGCGCATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACGGCAg  >  1:656982/1‑140 (MQ=255)

                                                                                                                                    agTTACGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACGGCAg  <  2:724168/139‑1 (MQ=255)

CAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATATCGACTACGAAACGGTCGGGCAAAACGGCAG  >  NC_000913/904596‑904867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A