Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F48 I1 R1
|
82 |
50.4 |
1709896 |
99.0% |
1692797 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
989,846 |
G→T |
L171M (CTG→ATG) |
pncB ← |
nicotinate phosphoribosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 989,846 | 0 | G | T | 100.0%
| 78.1
/ NA
| 23 | L171M (CTG→ATG) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/12); total (11/12) |
CATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTT > NC_000913/989712‑989965
|
cATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGa < 1:751368/140‑1 (MQ=255)
ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAgcg > 1:417577/1‑140 (MQ=255)
gCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAgcgc < 1:247235/140‑1 (MQ=255)
cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGcgcg > 2:666435/1‑140 (MQ=255)
gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGa < 2:336075/140‑1 (MQ=255)
gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATCGAAGCGCGa < 2:766300/140‑1 (MQ=255)
tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGAc > 1:249773/1‑140 (MQ=255)
gAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGCCGGGTGCCAAAATCCATCATATGGAAGCGCGACATATc < 2:414584/140‑1 (MQ=255)
aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAg > 2:305652/1‑140 (MQ=255)
gcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGt < 2:417577/140‑1 (MQ=255)
cTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTc < 1:666435/140‑1 (MQ=255)
ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGGTCACGCCCAGAAGTCgg > 1:834955/1‑139 (MQ=255)
ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGGTAACGCCGAGAAGTCgc > 1:319056/1‑139 (MQ=255)
ccACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGACTACTAAt > 1:375316/1‑140 (MQ=255)
gATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGGCGACTAATTTTCTTTTCAg > 1:223919/1‑140 (MQ=255)
aTTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGc < 1:305652/140‑1 (MQ=255)
cTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTgcg < 1:56058/140‑1 (MQ=255)
aTGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTc < 2:249773/140‑1 (MQ=255)
tttCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCGAGGGCTTGCGCAACGGCGGcc > 2:436031/1‑140 (MQ=255)
gTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGACTAATTTTCTTTTCAGCGTGTCGAGGGGTTGCGCAACGTCGGCCTGCGGt > 2:772951/1‑140 (MQ=255)
gagaAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag < 1:692971/140‑1 (MQ=255)
gaAAAACGGCGACGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTTCTTTCCAGCGGGTCGAGGGCTTGCGCAACGTCGGCCTGCGGGGagcgatagcg > 1:821064/1‑140 (MQ=255)
aCGGGTGCCAAAATCCATCATATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCAttt < 2:396871/140‑1 (MQ=255)
|
CATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTT > NC_000913/989712‑989965
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
