Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,082	C→A	D150Y (GAT→TAT)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,082	0	C	A	100.0%	377.4 / NA	105	D150Y (GAT→TAT)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (43/62); total (43/62)

Only 100 of 109 total aligned reads displayed.

GATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATG  >  NC_000913/82948‑83212

gATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCaa                                                                                                                               <  1:624758/140‑1 (MQ=255)

 aTGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGTACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAAt                                                                                                                              <  1:98462/140‑1 (MQ=255)

 aTGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAAt                                                                                                                              <  1:1288511/140‑1 (MQ=255)

 aTGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAAt                                                                                                                              <  2:283089/140‑1 (MQ=255)

 aTGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAAt                                                                                                                              <  2:1000369/140‑1 (MQ=255)

      aGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGgtg                                                                                                                         >  1:1444896/1‑140 (MQ=255)

            gcgTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGcc                                                                                                                   >  1:874353/1‑140 (MQ=255)

             cgTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGccc                                                                                                                  >  1:89063/1‑140 (MQ=255)

             cgTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGccc                                                                                                                  >  1:439422/1‑140 (MQ=255)

             cgTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGccc                                                                                                                  >  1:141636/1‑140 (MQ=255)

             cgTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGccc                                                                                                                  >  1:1042746/1‑140 (MQ=255)

                        gCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGc                                                                                                       >  2:654873/1‑140 (MQ=255)

                        gCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGc                                                                                                       >  2:808143/1‑140 (MQ=255)

                        gCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGc                                                                                                       >  1:707042/1‑140 (MQ=255)

                         ccGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCa                                                                                                      >  2:652891/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  1:344774/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  1:683507/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  2:52682/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  2:1273992/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  1:1101988/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  2:760065/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  2:277794/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  1:1310666/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  2:878097/1‑140 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGCGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCat                                                                                                     >  2:632580/1‑139 (MQ=255)

                          cGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAACCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCaa                                                                                                     >  1:499782/1‑140 (MQ=255)

                            cTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGa                                                                                                   >  2:1548298/1‑140 (MQ=255)

                            cTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGa                                                                                                   <  1:62232/140‑1 (MQ=255)

                            cTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGa                                                                                                   >  2:1399599/1‑140 (MQ=255)

                            cTGATGATCCCGGCGCACTCAAGCGGCAGGCCGTAGCCCACGGTGTGCGGAATGTTGGTGGTGGTGGCACCGGCATTAATCGCCTCTTCGACCACTCGCGCCAGATAGTCACTGAGTGTACGCCCTCCATCTTCGCAAGa                                                                                                   >  2:1459148/1‑140 (MQ=255)

                                     ccGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAc                                                                                          <  2:344774/140‑1 (MQ=255)

                                      cGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAca                                                                                         >  1:60516/1‑140 (MQ=255)

                                          gAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcatcat                                                                                     <  1:1470997/140‑1 (MQ=255)

                                          gAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcatcat                                                                                     <  1:845712/140‑1 (MQ=255)

                                          gAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcatcat                                                                                     <  1:52682/140‑1 (MQ=255)

                                          gAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcatcat                                                                                     <  1:1210634/140‑1 (MQ=255)

                                          gAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcatcat                                                                                     <  1:13102/140‑1 (MQ=255)

                                          gAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcatcat                                                                                     <  2:691557/140‑1 (MQ=255)

                                            aCTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCg                                                                                   <  1:552241/140‑1 (MQ=255)

                                            aCTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCg                                                                                   >  1:382358/1‑140 (MQ=255)

                                                         ggTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACggg                                                                      <  2:1387605/140‑1 (MQ=255)

                                                         ggTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACggg                                                                      <  2:439422/140‑1 (MQ=255)

                                                          gtgtAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgc                                                                     <  1:570365/140‑1 (MQ=255)

                                                           tgtAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgcg                                                                    >  1:526876/1‑140 (MQ=255)

                                                             tAGCCGACGGTGTCCGGAATGTTGATGGAGGCGCCTCCGACATTAACCGTCGCTTCGACCACTCGAACAAGGTAGGCAATGGGTGTGCGCCCGGCATCTTCGAAAGAAAATTCAACAGCATCGGTGTAATTACGGGcgcg                                                                  <  2:1492382/140‑1 (MQ=255)

                                                             tAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGcgcg                                                                  <  1:44505/140‑1 (MQ=255)

                                                             tAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGcgcg                                                                  <  1:325845/140‑1 (MQ=255)

                                                               gCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGtt                                                                <  2:1560724/140‑1 (MQ=255)

                                                               gCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGtt                                                                <  2:1444896/140‑1 (MQ=255)

                                                               gCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGtt                                                                <  2:707042/140‑1 (MQ=255)

                                                                cccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGttt                                                               <  2:486940/140‑1 (MQ=255)

                                                                cccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGttt                                                               <  1:1495775/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  1:654873/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:89063/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:141636/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:1408580/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  1:1205733/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  1:1273992/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:874353/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:1182886/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  1:1548298/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:382358/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  1:652891/140‑1 (MQ=255)

                                                                 ccACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                                                              <  2:526876/140‑1 (MQ=255)

                                                                  cACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCa                                                             >  1:1148845/1‑140 (MQ=255)

                                                                   aCGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCAc                                                            <  1:931526/140‑1 (MQ=255)

                                                                   aCGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCAc                                                            <  2:173651/140‑1 (MQ=255)

                                                                    cGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCAcc                                                           <  2:60516/140‑1 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGtt                                                                <  1:197230/134‑1 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGtt                                                                >  2:197230/1‑134 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCAcct                                                          >  2:893794/1‑139 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCa                                                          >  2:1182524/1‑140 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCa                                                          >  1:82331/1‑140 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCa                                                          >  1:816386/1‑140 (MQ=255)

                                                                     ggTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCa                                                          >  1:693839/1‑140 (MQ=255)

                                                                         tCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCAtata                                                      >  1:306180/1‑140 (MQ=255)

                                                                                tGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAgcg                                               <  2:82331/140‑1 (MQ=255)

                                                                                   tGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGt                                            >  2:1572302/1‑140 (MQ=255)

                                                                                         tggtggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATc                                      <  1:1572302/140‑1 (MQ=255)

                                                                                           gtggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAc                                    <  1:1395421/140‑1 (MQ=255)

                                                                                           gtggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAc                                    <  1:277794/140‑1 (MQ=255)

                                                                                            tggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAcc                                   >  2:291922/1‑140 (MQ=255)

                                                                                            tggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAcc                                   >  1:1402971/1‑140 (MQ=255)

                                                                                            tggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAcc                                   >  2:195404/1‑140 (MQ=255)

                                                                                              gCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTc                                 <  1:632580/140‑1 (MQ=255)

                                                                                              gCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTc                                 <  1:1182524/140‑1 (MQ=255)

                                                                                                 ccGGCATTAATCGCCGCTTCTACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATACATAGCGCGTTCGATCACCTCTTc                              >  2:705819/1‑140 (MQ=255)

                                                                                                     cATTAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGc                          >  2:1372105/1‑140 (MQ=255)

                                                                                                        tAATCGCCGCTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGtg                       <  2:487075/140‑1 (MQ=255)

                                                                                                             gccgcTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTgcg                  <  2:727624/140‑1 (MQ=255)

                                                                                                             gccgcTTCGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTgcg                  <  2:1042746/140‑1 (MQ=255)

                                                                                                                    cGACCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTtg           >  1:972872/1‑140 (MQ=255)

                                                                                                                      aCCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGtg                       >  1:270928/1‑126 (MQ=255)

                                                                                                                      aCCACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGtg                       <  2:270928/126‑1 (MQ=255)

                                                                                                                       ccACTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTtggtg        <  1:1095979/140‑1 (MQ=255)

                                                                                                                          cTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCg     <  1:808143/140‑1 (MQ=255)

                                                                                                                          cTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCg     <  1:127080/140‑1 (MQ=255)

                                                                                                                          cTCGCGCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACACCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCg     <  1:1580487/140‑1 (MQ=255)

                                                                                                                            cgcgCCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCGTGGTGGCGAt   <  2:306180/140‑1 (MQ=255)

                                                                                                                             gcgcCAGATAGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGAtg  <  1:1399599/140‑1 (MQ=255)

GATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATG  >  NC_000913/82948‑83212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A