Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	402,964	G→T	P406P (CCG→CCT)	phoA →	alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	402,964	0	G	T	100.0%	284.6 / NA	80	P406P (CCG→CCT)	phoA	alkaline phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (36/44); total (36/44)

TTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCGGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTT  >  NC_000913/402828‑403099

ttGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGc                                                                                                                                      <  1:1487316/140‑1 (MQ=255)

tcggCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGc                                                                                                                                      <  2:1334077/138‑1 (MQ=255)

       gaCGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCAccc                                                                                                                               <  2:1505965/140‑1 (MQ=255)

        aCGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCa                                                                                                                              >  1:190047/1‑140 (MQ=255)

         cGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAg                                                                                                                             <  1:124133/140‑1 (MQ=255)

              gATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCt                                                                                                                        <  1:1069028/140‑1 (MQ=255)

              gATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCt                                                                                                                        <  2:316831/140‑1 (MQ=255)

                tctcGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTaa                                                                                                                      <  2:1411110/140‑1 (MQ=255)

                tctcGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGACGGATACCAAAGCTCCTAGCCTCGCCCAGGCGCTaa                                                                                                                      <  2:116010/140‑1 (MQ=255)

                tctcGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGACACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTaa                                                                                                                      <  2:1475470/140‑1 (MQ=255)

                 ctcGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTaaa                                                                                                                     >  2:620643/1‑140 (MQ=255)

                 ctcGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTaaa                                                                                                                     <  2:190047/140‑1 (MQ=255)

                    gATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATAc                                                                                                                  >  2:1081420/1‑140 (MQ=255)

                      tGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCa                                                                                                                >  1:470619/1‑140 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            >  2:148679/1‑140 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            <  1:915457/140‑1 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            <  1:850831/140‑1 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            >  2:1481463/1‑140 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            <  1:620643/140‑1 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            >  1:451981/1‑140 (MQ=255)

                          gCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            >  1:1342307/1‑140 (MQ=255)

                          accGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTAATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGa                                                                                                            <  2:514913/139‑1 (MQ=255)

                              tACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGgc                                                                                                        >  1:1492781/1‑140 (MQ=255)

                              tACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGgc                                                                                                        >  2:269890/1‑140 (MQ=255)

                               aCAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGgcg                                                                                                       <  2:186377/140‑1 (MQ=255)

                                  aCGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAg                                                                                                    <  2:695590/140‑1 (MQ=255)

                                     ggCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtga                                                                                                 <  2:451981/140‑1 (MQ=255)

                                     ggCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtga                                                                                                 <  1:95394/140‑1 (MQ=255)

                                     ggCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtga                                                                                                 >  1:1471840/1‑140 (MQ=255)

                                      gcgcTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgat                                                                                                >  2:910028/1‑140 (MQ=255)

                                        gcTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGgcg                                                                                                       >  1:1554099/1‑131 (MQ=255)

                                        gcTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGgcg                                                                                                       <  2:1554099/131‑1 (MQ=255)

                                          tGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtg                                                                                            <  1:20793/140‑1 (MQ=255)

                                            gAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtgat                                                                                          >  1:536067/1‑140 (MQ=255)

                                            gAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtgat                                                                                          <  2:740243/140‑1 (MQ=255)

                                            gAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtgat                                                                                          <  2:17458/140‑1 (MQ=255)

                                            gAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtgat                                                                                          <  1:153196/140‑1 (MQ=255)

                                            gAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtgat                                                                                          <  2:536067/140‑1 (MQ=255)

                                            gAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAgtgatggtgat                                                                                          <  2:1159709/140‑1 (MQ=255)

                                                  gCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTa                                                                                    >  2:1497953/1‑140 (MQ=255)

                                                    tAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACg                                                                                  >  1:1293459/1‑140 (MQ=255)

                                                       aaaGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGa                                                                               <  2:1228954/140‑1 (MQ=255)

                                                       aaaGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGa                                                                               >  2:1168489/1‑140 (MQ=255)

                                                        aaGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGACGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGaa                                                                              <  2:1116537/140‑1 (MQ=255)

                                                              ggTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGa                                                                        >  1:1462487/1‑140 (MQ=255)

                                                               gTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGaa                                                                       <  2:1342307/140‑1 (MQ=255)

                                                               gTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGaa                                                                       <  2:1471840/140‑1 (MQ=255)

                                                                     cGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGa                                                                               >  1:685196/1‑126 (MQ=255)

                                                                     cGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGa                                                                               <  2:685196/126‑1 (MQ=255)

                                                                     cGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGAt                                                                 >  1:100012/1‑140 (MQ=255)

                                                                     cGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGAt                                                                 >  1:564950/1‑140 (MQ=255)

                                                                        tGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTca                                                              <  2:1293459/140‑1 (MQ=255)

                                                                        tGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTca                                                              <  1:910028/140‑1 (MQ=255)

                                                                          gTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCATCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAaga                                                                     <  2:716825/131‑1 (MQ=255)

                                                                          gTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAaga                                                                     >  1:716825/1‑131 (MQ=255)

                                                                                   aCCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATAc                                                   <  1:1081420/140‑1 (MQ=255)

                                                                                    ccGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATAcc                                                  >  1:1422321/1‑140 (MQ=255)

                                                                                    ccGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATAcc                                                  >  1:151013/1‑140 (MQ=255)

                                                                                     cGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCg                                                 >  1:987823/1‑140 (MQ=255)

                                                                                       cTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGc                                               <  2:470619/140‑1 (MQ=255)

                                                                                           tCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGcagtc                                           >  2:1579796/1‑140 (MQ=255)

                                                                                               gcccacgccAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGtt                                       <  2:1492781/140‑1 (MQ=255)

                                                                                               gcccacgccAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGtt                                       >  1:414920/1‑140 (MQ=255)

                                                                                               gcccacgccAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGtt                                       >  2:195027/1‑140 (MQ=255)

                                                                                               gcccacgccAGCCAGATTGTTGCGCCGGAGACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGtt                                       >  1:1400319/1‑140 (MQ=255)

                                                                                                    cgccAGCCAGATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTa                                  >  1:372176/1‑140 (MQ=255)

                                                                                                        agccagATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTgc                              <  2:334975/140‑1 (MQ=255)

                                                                                                         gccagATTGTTGCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTgcg                             >  1:1539331/1‑140 (MQ=255)

                                                                                                               ttgttgCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTAt                       <  1:269890/140‑1 (MQ=255)

                                                                                                                   tgCGCCGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGcc                   >  1:340966/1‑140 (MQ=255)

                                                                                                                       ccGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCa               <  1:469923/140‑1 (MQ=255)

                                                                                                                       ccGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCa               <  1:813093/140‑1 (MQ=255)

                                                                                                                       ccGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCa               <  2:467800/140‑1 (MQ=255)

                                                                                                                       ccGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCa               >  1:148399/1‑140 (MQ=255)

                                                                                                                       ccGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCa               >  1:286654/1‑140 (MQ=255)

                                                                                                                        cGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCAt              <  1:1168489/140‑1 (MQ=255)

                                                                                                                        cGGATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCACAt              <  2:414920/140‑1 (MQ=255)

                                                                                                                          gATACCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATgc            >  2:122381/1‑140 (MQ=255)

                                                                                                                             aCCAAAGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATgccgc         <  1:122381/140‑1 (MQ=255)

                                                                                                                                aaaGCTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCaa      <  2:1462487/140‑1 (MQ=255)

                                                                                                                                    cTCCTGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAAtgtt  >  2:98408/1‑140 (MQ=255)

TTGGCGAGACGGTCGATCTCGATGAAGCCGTACAACGGGCGCTGGAATTCGCTAAAAAGGAGGGTAACACGCTGGTCATAGTCACCGCTGATCACGCCCACGCCAGCCAGATTGTTGCGCCGGATACCAAAGCTCCGGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAACTCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTT  >  NC_000913/402828‑403099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A