Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	432,891	G→T	intergenic (‑40/‑111)	yajI ← / → nrdR	putative lipoprotein YajI/NrdR transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	432,891	0	G	T	100.0%	193.3 / NA	55	intergenic (‑40/‑111)	yajI/nrdR	putative lipoprotein YajI/NrdR transcriptional repressor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (24/31); total (24/31)

CTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGAGGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGACGCCGTATGCATTGCCCATTCTGTTTCG  >  NC_000913/432757‑433023

cTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTaa                                                                                                                                 >  1:269741/1‑140 (MQ=255)

cTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTaa                                                                                                                                 >  1:1546324/1‑140 (MQ=255)

  gtgtTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTaaaa                                                                                                                               >  2:1321335/1‑140 (MQ=255)

     tttCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAagca                                                                                                                            >  1:551375/1‑140 (MQ=255)

          tCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGaa                                                                                                                       <  1:1268711/140‑1 (MQ=255)

           cTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCGTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGaaa                                                                                                                      >  1:1172597/1‑140 (MQ=255)

             gTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGc                                                                                                                    <  1:325295/140‑1 (MQ=255)

               cGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCAt                                                                                                                  >  2:1176799/1‑140 (MQ=255)

                gCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATc                                                                                                                 <  2:742132/140‑1 (MQ=255)

                 cacttcacttTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCg                                                                                                                <  1:216373/140‑1 (MQ=255)

                     tcacttTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGCTAAAAGCAGCGAAAGCATCGATcc                                                                                                            >  2:1451173/1‑140 (MQ=255)

                              tgcACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCa                                                                                                   >  2:531466/1‑140 (MQ=255)

                                 acacaGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCaaaa                                                                                                >  2:1221869/1‑140 (MQ=255)

                                     aGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGt                                                                                            >  2:1127041/1‑140 (MQ=255)

                                     aGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGt                                                                                            >  1:717425/1‑140 (MQ=255)

                                     aGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGt                                                                                            >  1:1187885/1‑140 (MQ=255)

                                       gcgcTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTg                                                                                          <  2:416422/140‑1 (MQ=255)

                                       gcgcTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTg                                                                                          <  2:182724/140‑1 (MQ=255)

                                       gcgcTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTg                                                                                          >  2:1035126/1‑140 (MQ=255)

                                          cTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTgcgc                                                                                       <  2:269741/140‑1 (MQ=255)

                                          cTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTgcgc                                                                                       <  1:131463/140‑1 (MQ=255)

                                          cTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTgcgc                                                                                       <  2:524729/140‑1 (MQ=255)

                                          cTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTgcgc                                                                                       <  2:1108370/140‑1 (MQ=255)

                                                   aaCAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTtgttgt                                                                              <  2:1504741/140‑1 (MQ=255)

                                                   aaCAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTtgttgt                                                                              <  1:1176799/140‑1 (MQ=255)

                                                   aaCAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTtgttgt                                                                              <  2:551375/140‑1 (MQ=255)

                                                        gCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGt                                                                         <  2:1546324/140‑1 (MQ=255)

                                                             ccAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGtt                                                                    <  1:1035126/140‑1 (MQ=255)

                                                             ccAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGtt                                                                    <  2:1172597/140‑1 (MQ=255)

                                                             ccAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGtt                                                                    <  2:949999/140‑1 (MQ=255)

                                                             ccAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGtt                                                                    >  1:1304560/1‑140 (MQ=255)

                                                                  agGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTtgttgt                                                                              <  1:1114860/125‑1 (MQ=255)

                                                                  agGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTtgttgt                                                                              >  2:1114860/1‑125 (MQ=255)

                                                                  agGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAggg                                                               >  1:388820/1‑140 (MQ=255)

                                                                  agGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAggg                                                               <  1:1171520/140‑1 (MQ=255)

                                                                   ggAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGt                                                              >  2:613542/1‑140 (MQ=255)

                                                                      gCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTaaa                                                           >  1:1344637/1‑140 (MQ=255)

                                                                       cAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTaaaa                                                          <  1:552412/140‑1 (MQ=255)

                                                                          tCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAg                                                       >  2:1025163/1‑140 (MQ=255)

                                                                                    ttAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTa                                             <  2:578228/140‑1 (MQ=255)

                                                                                       gTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAacc                                          <  2:1448060/140‑1 (MQ=255)

                                                                                         aTTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAaccac                                        <  1:1127041/140‑1 (MQ=255)

                                                                                         aTTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAaccac                                        <  1:531466/140‑1 (MQ=255)

                                                                                         aTTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAaccac                                        <  1:1221869/140‑1 (MQ=255)

                                                                                              tAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGt                                   >  2:300230/1‑140 (MQ=255)

                                                                                                 gtcgtcCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAg                                >  2:332180/1‑140 (MQ=255)

                                                                                                 gtcgtcCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAg                                >  2:1470062/1‑140 (MQ=255)

                                                                                                  tcgtcCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAgg                               >  2:349167/1‑140 (MQ=255)

                                                                                                   cgtcCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGa                              <  2:388820/140‑1 (MQ=255)

                                                                                                    gtcCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTATAATAGATTTCCGTTAACCACCTGGTCAGGAc                             >  2:579349/1‑140 (MQ=255)

                                                                                                    gtcCCTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGAc                             <  2:717425/140‑1 (MQ=255)

                                                                                                       ccTTTCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCA‑CTGGTCAGGACGCCg                         <  1:360903/140‑1 (MQ=255)

                                                                                                         tttCTCGTCGCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGACGCCGt                        <  1:613542/140‑1 (MQ=255)

                                                                                                             tcgtcgCAATTAGCCTAATGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGACGCCGTATGc                    <  1:1470062/140‑1 (MQ=255)

                                                                                                                               tGGTAGATGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGACGCCGTATGCATTGCCCATTCTGTTTCg  <  2:1059463/140‑1 (MQ=255)

CTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAGAGGGTAAAAGCAGCGAAAGCATCGATCCTTTATCTCAAAAGCGTTGCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACCACCTGGTCAGGACGCCGTATGCATTGCCCATTCTGTTTCG  >  NC_000913/432757‑433023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A