Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F48 I1 R1	79	90.7	3162666	99.0%	3131039	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	705,290	G→C	V449V (GTG→GTC)	nagE →	N‑acetylglucosamine specific PTS enzyme IIABC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	705,290	0	G	C	100.0%	295.8 / NA	76	V449V (GTG→GTC)	nagE	N‑acetylglucosamine specific PTS enzyme IIABC component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (38/38); total (38/38)

ACAACCTGAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAACG  >  NC_000913/705154‑705426

aCAACCTGAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCAtt                                                                                                                                       >  2:170362/1‑140 (MQ=255)

   aCCTGAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCAttgtt                                                                                                                                    <  2:1233380/140‑1 (MQ=255)

    ccTGAAAGCGATTGACGCC‑GTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTgg                                                                                                                                  <  1:106164/140‑1 (MQ=255)

     cTGAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTgg                                                                                                                                  >  1:1149173/1‑140 (MQ=255)

       gAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGgcg                                                                                                                                <  1:170362/140‑1 (MQ=255)

       gAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGgcg                                                                                                                                <  2:78318/140‑1 (MQ=255)

        aaaGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGgcgc                                                                                                                               >  2:1532086/1‑140 (MQ=255)

          aGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGa                                                                                                                             <  2:692103/140‑1 (MQ=255)

          aGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGa                                                                                                                             <  2:958930/140‑1 (MQ=255)

              aTTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGc                                                                                                                         >  2:142216/1‑140 (MQ=255)

                       tGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCAt                                                                                                                <  1:1267885/140‑1 (MQ=255)

                          aTCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCgg                                                                                                             <  2:309545/140‑1 (MQ=255)

                             aCCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGgcga                                                                                                          <  1:180762/140‑1 (MQ=255)

                              cccGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGgcgat                                                                                                         >  2:679708/1‑140 (MQ=255)

                              cccGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGAATATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGgcgat                                                                                                         <  2:252469/140‑1 (MQ=255)

                              accGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGgcgat                                                                                                         <  2:1394377/139‑1 (MQ=255)

                                 gTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGgcgatgcg                                                                                                      <  1:1255301/140‑1 (MQ=255)

                                   cTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGgcgatgcgat                                                                                                    >  2:60710/1‑140 (MQ=255)

                                   cTGCGCCTTACAGTGGCTGACTCCGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTAAACAAAAAGACTATTCAGATCCTTGTTGGCGCGAAAGCAGAATCCATCGgcgatgcgat                                                                                                    >  2:1579804/1‑140 (MQ=255)

                                        ccTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATgaaga                                                                                               <  1:702276/140‑1 (MQ=255)

                                               gTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGt                                                                                        <  1:575133/140‑1 (MQ=255)

                                                  gCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGc                                                                                     >  1:429946/1‑140 (MQ=255)

                                                  gCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGc                                                                                     >  2:611167/1‑140 (MQ=255)

                                                   cTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGcc                                                                                    <  2:1149173/140‑1 (MQ=255)

                                                     gACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCg                                                                                  >  1:1101593/1‑140 (MQ=255)

                                                      aCTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGt                                                                                 <  2:81132/140‑1 (MQ=255)

                                                       ctctGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTg                                                                                <  1:1532086/140‑1 (MQ=255)

                                                         ctGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGt                                                                              <  1:502355/140‑1 (MQ=255)

                                                          tGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTc                                                                             >  1:213421/1‑140 (MQ=255)

                                                                      cgatacgatGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTg                                                                 <  2:1158686/140‑1 (MQ=255)

                                                                               gtgtAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTg                                                        >  1:956193/1‑140 (MQ=255)

                                                                                tgtAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGa                                                       >  1:964530/1‑140 (MQ=255)

                                                                                       gTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACt                                                <  2:964530/140‑1 (MQ=255)

                                                                                        tCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTc                                               >  2:653706/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  1:1013/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  1:820875/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  1:1194704/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  2:482576/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  2:1120963/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  2:1124895/1‑140 (MQ=255)

                                                                                          tGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCg                                             >  1:1254734/1‑140 (MQ=255)

                                                                                            ggTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGc                                           >  2:656133/1‑140 (MQ=255)

                                                                                              tGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCaa                                         <  1:60710/140‑1 (MQ=255)

                                                                                                 ttCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTg                                      >  2:42188/1‑140 (MQ=255)

                                                                                                  tCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGc                                     >  2:1037098/1‑140 (MQ=255)

                                                                                                    tGGGGTAGTGAAGCTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCTGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCcg                                   <  2:956193/140‑1 (MQ=255)

                                                                                                    tGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCAAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCcg                                   <  2:492294/140‑1 (MQ=255)

                                                                                                      gggTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCcgcg                                 >  1:564056/1‑140 (MQ=255)

                                                                                                      gggTAGTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCcgcg                                 >  1:940993/1‑140 (MQ=255)

                                                                                                           gTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGTTGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCTGCTGAAGCAACTTCGGCAACTGCCGCGCCTGt                            >  2:66439/1‑140 (MQ=255)

                                                                                                           gTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCcg                                   <  1:1507941/133‑1 (MQ=255)

                                                                                                           gTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCcg                                   >  2:1507941/1‑133 (MQ=255)

                                                                                                           gTGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGt                            >  2:910292/1‑140 (MQ=255)

                                                                                                            tGAAACTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTa                           >  2:1423591/1‑140 (MQ=255)

                                                                                                               aaCTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCa                        <  1:577455/140‑1 (MQ=255)

                                                                                                                aCTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCaa                       >  2:1328958/1‑140 (MQ=255)

                                                                                                                aCTGAACAAACAGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCaa                       >  2:1417028/1‑140 (MQ=255)

                                                                                                                    aacaaacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTTCGGCAACTGCCGCGCCTGTAGCAAAAcc                   >  2:1364492/1‑140 (MQ=255)

                                                                                                                     acaaacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCg                  <  2:1013/140‑1 (MQ=255)

                                                                                                                     acaaacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCg                  <  1:482576/140‑1 (MQ=255)

                                                                                                                     acaaacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAAAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCg                  <  1:910292/140‑1 (MQ=255)

                                                                                                                     acaaacaGACTATTAAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCAATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCg                  <  2:489006/140‑1 (MQ=255)

                                                                                                                      caaacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGc                 >  2:1178311/1‑140 (MQ=255)

                                                                                                                       aaacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCa                <  2:564056/140‑1 (MQ=255)

                                                                                                                        aacaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAg               >  2:1210229/1‑140 (MQ=255)

                                                                                                                         acaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAgg              >  1:760596/1‑140 (MQ=255)

                                                                                                                         acaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAgg              <  1:142216/140‑1 (MQ=255)

                                                                                                                         acaGACTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAgg              <  1:1423591/140‑1 (MQ=255)

                                                                                                                             aCTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGt          <  1:42188/140‑1 (MQ=255)

                                                                                                                              cTATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTa         >  2:1182979/1‑140 (MQ=255)

                                                                                                                               tATTCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTAc        <  1:611167/140‑1 (MQ=255)

                                                                                                                                 ttCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCa      >  1:325144/1‑140 (MQ=255)

                                                                                                                                 ttCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCa      <  2:1532061/140‑1 (MQ=255)

                                                                                                                                  tCAGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCaa     >  2:1472954/1‑140 (MQ=255)

                                                                                                                                    aGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCaatc   <  1:656133/140‑3 (MQ=255)

                                                                                                                                    aGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAAc   <  1:1234810/140‑1 (MQ=255)

                                                                                                                                    aGGTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAAc   <  1:741718/140‑1 (MQ=255)

                                                                                                                                     ggTCATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAAcg  <  1:478528/140‑1 (MQ=255)

ACAACCTGAAAGCGATTGACGCCTGTATCACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAACG  >  NC_000913/705154‑705426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A