Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F48 I1 R1	62	93.4	3176452	99.2%	3151040	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,895	C→A	A37S (GCC→TCC)	yaeF ←	peptidase C92 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,895	0	C	A	100.0%	337.0 / NA	95	A37S (GCC→TCC)	yaeF	peptidase C92 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (50/45); total (50/45)

CAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGG  >  NC_000913/216760‑217029

cAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCt                                                                                                                                    <  1:693786/140‑1 (MQ=255)

cAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCt                                                                                                                                    <  1:1318735/140‑1 (MQ=255)

      aGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTActgct                                                                                                                              >  1:303964/1‑140 (MQ=255)

         ttACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCtgtg                                                                                                                           <  1:612731/140‑1 (MQ=255)

          tACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTgg                                                                                                                          >  1:747422/1‑140 (MQ=255)

            ccccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCt                                                                                                                        >  2:656903/1‑140 (MQ=255)

             cccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTg                                                                                                                       <  1:1190428/140‑1 (MQ=255)

                 aGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGTTgg                                                                                                                   >  2:76516/1‑140 (MQ=255)

                 aGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCT‑GAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATggg                                                                                                                  >  2:942672/1‑140 (MQ=255)

                  ggCTTGAGGAGAACAGCAAATCGCCGGGTATGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATggg                                                                                                                  >  1:1404301/1‑140 (MQ=255)

                      tGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctg                                                                                                              >  1:290472/1‑140 (MQ=255)

                       gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTGTTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTTGGAATCTACTGCTGTGGCTGATGGGTctgg                                                                                                             >  1:324249/1‑140 (MQ=255)

                       gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctgg                                                                                                             <  1:1248610/140‑1 (MQ=255)

                       gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctgg                                                                                                             >  2:1568595/1‑140 (MQ=255)

                       gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctgg                                                                                                             >  1:1115635/1‑140 (MQ=255)

                       gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctgg                                                                                                             >  2:1176045/1‑140 (MQ=255)

                       gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctgg                                                                                                             >  2:856528/1‑140 (MQ=255)

                        aggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctggc                                                                                                            <  1:1248493/140‑1 (MQ=255)

                        aggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctggc                                                                                                            <  1:1494105/140‑1 (MQ=255)

                           agaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTGTTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTTGGAATCTACTGCTGTGGCTGATGGGTctggctgg                                                                                                         <  2:324249/140‑1 (MQ=255)

                           agaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctggctgg                                                                                                         <  2:1115635/140‑1 (MQ=255)

                           agaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctggctgg                                                                                                         <  1:656903/140‑1 (MQ=255)

                           agaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTctggctgg                                                                                                         <  2:899588/140‑1 (MQ=255)

                             aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTggctggct                                                                                                       >  1:996214/1‑140 (MQ=255)

                             aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTggctggct                                                                                                       >  2:436878/1‑140 (MQ=255)

                             aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTggctggct                                                                                                       >  2:1014626/1‑140 (MQ=255)

                             aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTggctggct                                                                                                       >  2:210703/1‑140 (MQ=255)

                             aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTggctggct                                                                                                       <  1:1154854/140‑1 (MQ=255)

                                aGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGat                                                                                                    <  2:1255120/140‑1 (MQ=255)

                                  cAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGatat                                                                                                  >  2:1285767/1‑140 (MQ=255)

                                     aTCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCaa                                                                                               <  1:948530/140‑1 (MQ=255)

                                     aTCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCaa                                                                                               >  2:543987/1‑140 (MQ=255)

                                     aTCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCaa                                                                                               <  2:402757/140‑1 (MQ=255)

                                       cGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCaa                                                                                               >  2:1443570/1‑138 (MQ=255)

                                       cGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCaa                                                                                               <  1:1443570/138‑1 (MQ=255)

                                       cGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACg                                                                                             >  2:491552/1‑140 (MQ=255)

                                       cGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACg                                                                                             >  1:204565/1‑140 (MQ=255)

                                                tGAGATCAGGTGCCGTTTTTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGTCAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATAATCGGTGCAAGcc                                                                                    <  2:1008924/140‑1 (MQ=255)

                                                tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCT‑GAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCg                                                                                   <  1:942672/140‑1 (MQ=255)

                                                  agaTCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGa                                                                                  >  2:1137627/1‑140 (MQ=255)

                                                     tCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAg                                                                               <  2:1404301/140‑1 (MQ=255)

                                                       aGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTa                                                                             >  1:493412/1‑140 (MQ=255)

                                                        ggTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTaa                                                                            >  2:478370/1‑140 (MQ=255)

                                                        ggTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTaa                                                                            >  1:1411164/1‑140 (MQ=255)

                                                         gTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTaaa                                                                           >  2:168028/1‑140 (MQ=255)

                                                            ccGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAaaaga                                                                        >  2:1124499/1‑140 (MQ=255)

                                                               ttATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAaaagaaag                                                                     <  2:906776/140‑1 (MQ=255)

                                                               ttATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAaaagaaag                                                                     <  2:303964/140‑1 (MQ=255)

                                                                tATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGc                                                                    <  1:1124499/140‑1 (MQ=255)

                                                                tATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGc                                                                    <  1:448240/140‑1 (MQ=255)

                                                                 aTTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCt                                                                   >  1:1485570/1‑140 (MQ=255)

                                                                 aTTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCt                                                                   >  2:1068956/1‑140 (MQ=255)

                                                                   ttCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTgg                                                                 >  1:161859/1‑140 (MQ=255)

                                                                    tCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTggg                                                                >  2:1133742/1‑140 (MQ=255)

                                                                       tttATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGgag                                                             >  1:599204/1‑140 (MQ=255)

                                                                                tgTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAggcg                                                    >  1:1005351/1‑140 (MQ=255)

                                                                                tgTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAggcg                                                    >  1:22155/1‑140 (MQ=255)

                                                                                tgTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAggcg                                                    >  1:260359/1‑140 (MQ=255)

                                                                                tgTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAggcg                                                    >  1:656113/1‑140 (MQ=255)

                                                                                  ttCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAggcggc                                                  >  1:293230/1‑140 (MQ=255)

                                                                                  ttCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAggcggc                                                  >  2:521126/1‑140 (MQ=255)

                                                                                     ggTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGt                                               >  1:1294421/1‑140 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATTTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  1:896494/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  2:88915/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  2:161859/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  2:996214/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  1:1176045/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  1:325659/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  1:491552/140‑1 (MQ=255)

                                                                                      gTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTa                                              <  2:1144850/140‑1 (MQ=255)

                                                                                       tGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTAc                                             >  2:818193/1‑140 (MQ=255)

                                                                                           agagCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCt                                         <  1:76516/140‑1 (MQ=255)

                                                                                           agagCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCt                                         <  1:776054/140‑1 (MQ=255)

                                                                                            gagCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCtt                                        >  2:813801/1‑140 (MQ=255)

                                                                                                tttGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGt                                    <  1:1133742/140‑1 (MQ=255)

                                                                                                 ttGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGtt                                   >  2:377627/1‑140 (MQ=255)

                                                                                                 ttGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGtt                                   >  2:57761/1‑140 (MQ=255)

                                                                                                 ttGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGtt                                   >  1:1567402/1‑140 (MQ=255)

                                                                                                 ttGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGtt                                   >  2:175501/1‑140 (MQ=255)

                                                                                                 ttGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGtt                                   >  2:1408358/1‑140 (MQ=255)

                                                                                                    aTGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAt                                <  2:1005351/140‑1 (MQ=255)

                                                                                                    aTGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAt                                <  2:1221571/140‑1 (MQ=255)

                                                                                                    aTGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAt                                <  2:493412/140‑1 (MQ=255)

                                                                                                    aTGCTGGAATTTGACAGCCAATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGTTGGCTGATATCCGCGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAt                                <  2:175880/140‑1 (MQ=255)

                                                                                                        tGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAt                                <  2:1402326/136‑1 (MQ=255)

                                                                                                        tGGAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAt                                >  1:1402326/1‑136 (MQ=255)

                                                                                                         ggAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCAtt                           <  1:168028/140‑1 (MQ=255)

                                                                                                         ggAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCAtt                           <  1:987807/140‑1 (MQ=255)

                                                                                                         ggAATTTGACAGCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCAtt                           <  2:260359/140‑1 (MQ=255)

                                                                                                         ggAATTTGACAGCCCATGTTTTTGCCTCGAAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCAtt                           <  2:429392/140‑1 (MQ=255)

                                                                                                                    gCCCATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATcc                >  1:1529610/1‑140 (MQ=255)

                                                                                                                     cccATGTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTTGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACATGCTTCAGTACGCCTTTGGTTTATCTATTTTATTCAAtgct               >  2:68601/1‑137 (MQ=255)

                                                                                                                          gTTTTTGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTaa          >  1:1067557/1‑140 (MQ=255)

                                                                                                                               tGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTaaaaaag     <  1:1014626/140‑2 (MQ=255)

                                                                                                                               tGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTaaaaaaa     >  2:1290022/1‑140 (MQ=255)

                                                                                                                               tGCCTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTaaaaaaa     <  2:1294421/140‑1 (MQ=255)

                                                                                                                                  cTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAggg  <  2:747422/140‑1 (MQ=255)

                                                                                                                                  cTCGGAATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAggg  <  1:521126/140‑1 (MQ=255)

CAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGG  >  NC_000913/216760‑217029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A