Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F48 I1 R1	70	151.6	5072932	98.7%	5006983	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,827	A→T	D81E (GAT→GAA)	yaeI ←	phosphodiesterase YaeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,827	0	A	T	100.0%	372.2 / NA	104	D81E (GAT→GAA)	yaeI	phosphodiesterase YaeI
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (55/49); total (55/49)

Only 100 of 106 total aligned reads displayed.

TACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGA  >  NC_000913/184691‑184962

tACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCg                                                                                                                                       <  1:568248/140‑1 (MQ=255)

tACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCg                                                                                                                                       <  1:211377/140‑1 (MQ=255)

tACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCg                                                                                                                                       <  1:862139/140‑1 (MQ=255)

tACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCg                                                                                                                                       <  1:400310/140‑1 (MQ=255)

 aCCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCgg                                                                                                                                      >  2:1220048/1‑140 (MQ=255)

  ccAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCggg                                                                                                                                     <  2:2481224/140‑1 (MQ=255)

     aCAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCtt                                                                                                                                  >  2:1149694/1‑140 (MQ=255)

      cAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATATGTAATCGCCGCCCAGTAATATCAATTCGGGCttt                                                                                                                                 <  2:1449814/140‑1 (MQ=255)

        gggCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCttttt                                                                                                                               >  2:787219/1‑139 (MQ=255)

         ggCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGt                                                                                                                              <  2:932654/140‑1 (MQ=255)

          gCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGtt                                                                                                                             >  1:524142/1‑140 (MQ=255)

          gCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGtt                                                                                                                             >  1:115176/1‑140 (MQ=255)

          gCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGtt                                                                                                                             >  1:94155/1‑140 (MQ=255)

          gCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGtt                                                                                                                             >  1:1498045/1‑140 (MQ=255)

          gCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGtt                                                                                                                             >  1:1651494/1‑140 (MQ=255)

           cGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTc                                                                                                                            <  2:524142/140‑1 (MQ=255)

            gATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCt                                                                                                                           <  1:1149694/140‑1 (MQ=255)

            gATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCt                                                                                                                           >  1:1831153/1‑140 (MQ=255)

               cGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATg                                                                                                                        <  1:993912/140‑1 (MQ=255)

                 tGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGcc                                                                                                                      <  2:1104095/140‑1 (MQ=255)

                 tGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGcc                                                                                                                      <  2:1672454/140‑1 (MQ=255)

                   gTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCaa                                                                                                                    >  2:1772338/1‑140 (MQ=255)

                          aaGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAAt                                                                                                            <  1:360141/140‑1 (MQ=255)

                             cAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATcgc                                                                                                         >  1:755792/1‑140 (MQ=255)

                                gCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTc                                                                                                      >  1:240699/1‑140 (MQ=255)

                                       tCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATc                                                                                               <  2:240699/140‑1 (MQ=255)

                                        cGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCa                                                                                              <  1:2216266/140‑1 (MQ=255)

                                         ggCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAg                                                                                             <  2:1390606/140‑1 (MQ=255)

                                         ggCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAg                                                                                             >  1:670840/1‑140 (MQ=255)

                                          gcgcACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAgt                                                                                            >  2:1929427/1‑139 (MQ=255)

                                          gcgcACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAgg                                                                                            >  2:1268794/1‑140 (MQ=255)

                                          gcgcACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAgg                                                                                            <  1:787219/140‑1 (MQ=255)

                                          gcgcACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAgg                                                                                            >  1:131708/1‑140 (MQ=255)

                                            gcACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCt                                                                                          <  2:1234614/140‑1 (MQ=255)

                                                aTTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTaaa                                                                                      <  1:570557/140‑1 (MQ=255)

                                                    ggCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGaa                                                                                  <  2:94155/140‑1 (MQ=255)

                                                    ggCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGaa                                                                                  <  1:360418/140‑1 (MQ=255)

                                                    ggCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGaa                                                                                  <  1:2376741/140‑1 (MQ=255)

                                                     gCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAAc                                                                                 <  1:436917/140‑1 (MQ=255)

                                                     gCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAAc                                                                                 <  1:2514152/140‑1 (MQ=255)

                                                         ggggggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACaaaa                                                                             >  2:888072/1‑140 (MQ=255)

                                                          gggggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc                                                                            >  1:1408264/1‑140 (MQ=255)

                                                          gggggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc                                                                            >  2:11301/1‑140 (MQ=255)

                                                          gggggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAAc                                                                            <  2:755792/140‑1 (MQ=255)

                                                          gggggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCACGTCAGAAATCAGGCTTAAAGGAACAAAAc                                                                            <  1:991009/140‑1 (MQ=255)

                                                            gggATAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga                                                                          <  2:2147433/140‑1 (MQ=255)

                                                            gggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga                                                                          <  1:2265503/140‑1 (MQ=255)

                                                            gggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga                                                                          <  2:401400/140‑1 (MQ=255)

                                                            gggAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACga                                                                          <  2:817497/140‑1 (MQ=255)

                                                              gagaGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACgaga                                                                        <  2:1207602/140‑1 (MQ=255)

                                                              aagagTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACgaga                                                                        <  2:131708/139‑1 (MQ=255)

                                                               agagTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGagag                                                                       <  2:1498045/140‑1 (MQ=255)

                                                                    aCGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATg                                                                  >  1:267090/1‑140 (MQ=255)

                                                                    aCGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATg                                                                  >  1:1452120/1‑140 (MQ=255)

                                                                    aCGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATg                                                                  >  1:695788/1‑140 (MQ=255)

                                                                     cGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATgg                                                                 >  1:1886376/1‑140 (MQ=255)

                                                                      gTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGga                                                                >  1:640402/1‑140 (MQ=255)

                                                                         aCTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGAt                                                             <  1:1543988/140‑1 (MQ=255)

                                                                           tAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCg                                                           >  2:1507969/1‑140 (MQ=255)

                                                                           tAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCg                                                           >  1:2341783/1‑140 (MQ=255)

                                                                             aaCGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGc                                                         >  2:124008/1‑140 (MQ=255)

                                                                             aaCGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGc                                                         <  1:1696778/140‑1 (MQ=255)

                                                                             aaCGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGc                                                         >  2:904458/1‑140 (MQ=255)

                                                                                    aaaaaaTCAACGGAATATTAAAAAATAAGTAAACCGCGGCCAAGAAAAACAAATCGGGCTTTTTTTCTAAGGCAAGGAGC‑ATCGCGTTAGAAATCAAGCTTAAAGGAACAAAACGAAAGTAATGGGGGACCGGCAGaaaa                                                  <  2:1831153/140‑1 (MQ=255)

                                                                                    aaaaaTTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCACaaaa                                                  >  2:717518/1‑140 (MQ=255)

                                                                                     aaaaTTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGCCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGaaaaa                                                 >  1:1358469/1‑140 (MQ=255)

                                                                                          tCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAAtt                                            <  2:1358469/140‑1 (MQ=255)

                                                                                              cGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGa                                        >  1:275193/1‑140 (MQ=255)

                                                                                               gACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGaa                                       <  2:267090/140‑1 (MQ=255)

                                                                                               gACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGaa                                       <  2:670840/140‑1 (MQ=255)

                                                                                               aaCATATCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAAAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGaa                                       <  2:1355108/139‑1 (MQ=255)

                                                                                                   tatCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGt                                   <  2:1651494/140‑1 (MQ=255)

                                                                                                    atCAAATAATACGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGtg                                  >  1:1094967/1‑140 (MQ=255)

                                                                                                          taataCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGt                            <  1:777436/140‑1 (MQ=255)

                                                                                                          taataCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGt                            <  1:1929427/140‑1 (MQ=255)

                                                                                                           aataCGTAATCGCCGCCCAGTAATATCAATTCTGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGtt                           >  1:1509827/1‑140 (MQ=255)

                                                                                                             taCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGt                         >  1:544642/1‑140 (MQ=255)

                                                                                                             taCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGt                         >  1:77651/1‑140 (MQ=255)

                                                                                                             taCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGt                         >  1:859428/1‑140 (MQ=255)

                                                                                                             taCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGt                         >  2:43012/1‑140 (MQ=255)

                                                                                                             taCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGt                         >  2:2413997/1‑140 (MQ=255)

                                                                                                             taCGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGt                         >  2:1544153/1‑140 (MQ=255)

                                                                                                               cGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCt                       >  2:23015/1‑140 (MQ=255)

                                                                                                               cGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCt                       >  1:594368/1‑140 (MQ=255)

                                                                                                               cGTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCt                       >  1:1845727/1‑140 (MQ=255)

                                                                                                                gTAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCtt                      >  1:1243566/1‑140 (MQ=255)

                                                                                                                 tAATCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCttt                     >  2:2329606/1‑140 (MQ=255)

                                                                                                                   aTCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCtt                      <  1:1175002/137‑1 (MQ=255)

                                                                                                                   aTCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCtt                      >  2:1175002/1‑137 (MQ=255)

                                                                                                                   aTCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaa                   >  1:124624/1‑140 (MQ=255)

                                                                                                                   aTCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaa                   >  1:894968/1‑140 (MQ=255)

                                                                                                                    tCGCCGCCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaat                  >  2:1667624/1‑139 (MQ=255)

                                                                                                                     cgccgcCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGATAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaaaa                 >  2:1902721/1‑140 (MQ=255)

                                                                                                                     cgccgcCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaaaa                 >  2:409707/1‑140 (MQ=255)

                                                                                                                     cgccgcCCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaaaa                 >  2:254594/1‑140 (MQ=255)

                                                                                                                        cgccCAGTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTaaaaaat              >  2:1217043/1‑139 (MQ=255)

                                                                                                                              gTAATATCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAaggcgagg        <  1:957144/140‑1 (MQ=255)

                                                                                                                                    tCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCTCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGa  >  1:1193141/1‑140 (MQ=255)

                                                                                                                                    tCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGa  <  2:1094967/140‑1 (MQ=255)

                                                                                                                                    tCAATTCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGa  <  1:127966/140‑1 (MQ=255)

TACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAATATCAAATCGGGCTTTTGTTCTATGCCAA‑GAGCAATCGCGTCAGAAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGA  >  NC_000913/184691‑184962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A