Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F48 I1 R1 70 151.6 5072932 98.7% 5006983 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 794,131 G→T L172M (CTG→ATG)  modE ← DNA‑binding transcriptional dual regulator ModE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,1310GT99.4% 570.3 / ‑4.0 160L172M (CTG→ATG) modEDNA‑binding transcriptional dual regulator ModE
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (89/70);  total (90/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 166 total aligned reads displayed.

GCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCG  >  NC_000913/793994‑794267
                                                                                                                                         |                                                                                                                                        
gCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCatccatcc                                                                                                                                        >  1:108081/1‑140 (MQ=255)
  aCTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATcccc                                                                                                                                      >  2:1291901/1‑140 (MQ=255)
           cgccgcGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGAcgcgcg                                                                                                                             <  2:199125/140‑1 (MQ=255)
            gccgcGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACgcgcgc                                                                                                                            <  2:658477/140‑1 (MQ=255)
              cgcgCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGcgccg                                                                                                                          <  2:1822172/140‑1 (MQ=255)
                cgcTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCt                                                                                                                        >  2:2415969/1‑140 (MQ=255)
                cgcTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCt                                                                                                                        >  2:608994/1‑140 (MQ=255)
                cgcTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGCCCTGTGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCt                                                                                                                        >  2:1640264/1‑140 (MQ=255)
                 gcTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCtt                                                                                                                       <  2:2141060/140‑1 (MQ=255)
                 gcTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCtt                                                                                                                       >  1:2478372/1‑140 (MQ=255)
                              aataatACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTg                                                                                                          <  1:737541/140‑1 (MQ=255)
                              aataatACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTg                                                                                                          <  1:423430/140‑1 (MQ=255)
                              aataatACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTg                                                                                                          <  1:1372826/140‑1 (MQ=255)
                               ataataCCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACTCTTCTTTGCCTTCATCCATCCCCAGACTCGCGCCGCTTTGTACGTTAATTGc                                                                                                         >  2:771475/1‑140 (MQ=255)
                               ataataCCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCACCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGc                                                                                                         >  2:2311610/1‑140 (MQ=255)
                                 aataCCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGa                                                                                                       >  2:2462829/1‑140 (MQ=255)
                                 aataCCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGa                                                                                                       >  1:2302979/1‑140 (MQ=255)
                                     cccGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACttt                                                                                                   >  1:1177044/1‑140 (MQ=255)
                                      ccGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTc                                                                                                  <  2:1307196/140‑1 (MQ=255)
                                      ccGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTc                                                                                                  <  2:789724/140‑1 (MQ=255)
                                       cGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCa                                                                                                 >  2:8673/1‑140 (MQ=255)
                                       cGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCa                                                                                                 >  1:361601/1‑140 (MQ=255)
                                         gTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAgg                                                                                               >  1:226698/1‑140 (MQ=255)
                                         gTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAgg                                                                                               <  2:2026443/140‑1 (MQ=255)
                                          tAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGc                                                                                              >  1:1502100/1‑140 (MQ=255)
                                          tAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGc                                                                                              >  2:1812923/1‑140 (MQ=255)
                                          tAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGc                                                                                              >  2:391408/1‑140 (MQ=255)
                                           aaTTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCg                                                                                             >  2:515731/1‑140 (MQ=255)
                                             ttGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCgtt                                                                                           >  2:875982/1‑139 (MQ=255)
                                              tGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCgtgt                                                                                          >  1:1300960/1‑140 (MQ=255)
                                              tGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCgtgt                                                                                          >  2:2270329/1‑140 (MQ=255)
                                                    tCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTcc                                                                                    >  2:171449/1‑140 (MQ=255)
                                                        ggTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCa                                                                                <  2:691970/139‑1 (MQ=255)
                                                        cGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCa                                                                                <  1:2410117/140‑1 (MQ=255)
                                                        cGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCa                                                                                >  1:499334/1‑140 (MQ=255)
                                                           tttGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGcc                                                                             <  1:1179612/140‑1 (MQ=255)
                                                           tttGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGcc                                                                             <  1:1762097/140‑1 (MQ=255)
                                                             tGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGTGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCat                                                                           >  2:1609400/1‑139 (MQ=255)
                                                             tGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAg                                                                           >  1:816786/1‑140 (MQ=255)
                                                              gcgcGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGt                                                                          <  2:1502100/140‑1 (MQ=255)
                                                              gcgcGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGt                                                                          <  1:2501465/140‑1 (MQ=255)
                                                                  gACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGa                                                                      <  2:1448432/140‑1 (MQ=255)
                                                                   aCCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGAc                                                                     >  1:1019660/1‑140 (MQ=255)
                                                                    ccGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACa                                                                    <  1:2520372/140‑1 (MQ=255)
                                                                     cGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACAt                                                                   <  1:1537928/140‑1 (MQ=255)
                                                                       ccTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCa                                                                 <  2:431574/140‑1 (MQ=255)
                                                                       ccTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCa                                                                 >  2:1524625/1‑140 (MQ=255)
                                                                            tCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATg                                                            <  1:1630723/140‑1 (MQ=255)
                                                                                 aGTAATACCTACCCACGGCGCTTTTAGCAATATCACCACTTCTTTGACTTCATCCATCCCCAGACCCGCGCCGCTTTGCGCGGTAATTGCGACTTTCAGACGTGTTTTTCCTTCAGCCAGCACGACATCAACATGCtttt                                                       >  2:1167064/1‑140 (MQ=255)
                                                                                 aGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGtt                                                       <  1:2428728/140‑1 (MQ=255)
                                                                                  gTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGTGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTg                                                      <  2:1116871/140‑1 (MQ=255)
                                                                                   tAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGa                                                     <  1:8673/140‑1 (MQ=255)
                                                                                   tAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGa                                                     <  2:2045908/140‑1 (MQ=255)
                                                                                    aaTACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGaa                                                    <  1:785794/140‑1 (MQ=255)
                                                                                      tacctaccCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACg                                                  >  1:269929/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTCAGACATCAACATGCTGTTGAACGtcatc                                             >  2:2374199/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  2:571181/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  2:463018/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  2:92713/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  2:1419549/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  1:1421795/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  2:1275965/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  1:2186894/1‑140 (MQ=255)
                                                                                           accCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCCTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatc                                             >  2:1262623/1‑140 (MQ=255)
                                                                                                cGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGAt                                        >  2:746294/1‑140 (MQ=255)
                                                                                                cGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGAt                                        >  2:2514168/1‑140 (MQ=255)
                                                                                                 ggCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATc                                       >  2:1744368/1‑140 (MQ=255)
                                                                                                 ggCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATc                                       >  1:2338772/1‑140 (MQ=255)
                                                                                                  gcgcTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATcg                                      >  1:31531/1‑140 (MQ=255)
                                                                                                   cgcTTTTAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATcgc                                     >  2:216701/1‑140 (MQ=255)
                                                                                                       tttAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCgcgggc                                 <  1:41843/140‑1 (MQ=255)
                                                                                                       tttAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCgcgggc                                 <  2:150590/140‑1 (MQ=255)
                                                                                                       tttAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCgcgggc                                 <  1:608994/140‑1 (MQ=255)
                                                                                                       tttAGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCgcgggc                                 <  2:1233539/140‑1 (MQ=255)
                                                                                                          aGCAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGt                              <  2:247793/140‑1 (MQ=255)
                                                                                                            cAATATCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGa                            <  1:1628572/140‑1 (MQ=255)
                                                                                                               tatCAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATgg                         <  2:1073499/140‑1 (MQ=255)
                                                                                                                  cAACACTTCTTTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTAc                      <  1:515731/140‑1 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAAccc               >  2:1257203/1‑139 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCa               <  1:469252/140‑1 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCa               <  1:1822599/140‑1 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCa               <  2:1931452/140‑1 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCa               <  2:294460/140‑1 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCa               >  1:2094098/1‑140 (MQ=255)
                                                                                                                         tcttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCa               >  2:1721425/1‑140 (MQ=255)
                                                                                                                          cttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCAc              >  1:968058/1‑140 (MQ=255)
                                                                                                                          cttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCAc              >  2:1361251/1‑140 (MQ=255)
                                                                                                                          cttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCAc              >  2:486180/1‑140 (MQ=255)
                                                                                                                          cttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCAc              >  2:43986/1‑140 (MQ=255)
                                                                                                                          cttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCAc              >  1:393166/1‑140 (MQ=255)
                                                                                                                          cttTGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCAc              >  2:363747/1‑140 (MQ=255)
                                                                                                                             tGCCTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTgg           >  1:155644/1‑140 (MQ=255)
                                                                                                                               ccTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAATATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGtt         <  1:1721425/140‑1 (MQ=255)
                                                                                                                               ccTTCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGtt         <  2:1177044/140‑1 (MQ=255)
                                                                                                                                 ttCATCCATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTAc       <  2:155644/140‑1 (MQ=255)
                                                                                                                                    atccatccCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACggg    >  1:2335586/1‑140 (MQ=255)
                                                                                                                                    atccatccCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACggg    <  1:571181/140‑1 (MQ=255)
                                                                                                                                    atccatccCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACggg    >  1:688388/1‑140 (MQ=255)
                                                                                                                                      ccATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGgcg  <  1:2462829/140‑1 (MQ=255)
                                                                                                                                      ccATCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGgcg  <  1:1348436/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCG  >  NC_000913/793994‑794267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A