Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A20 F48 I1 R1 52 61.9 2103308 98.8% 2078068 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 794,407 G→T R80S (CGC→AGC)  modE ← DNA‑binding transcriptional dual regulator ModE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,4070GT100.0% 154.1 / NA 44R80S (CGC→AGC) modEDNA‑binding transcriptional dual regulator ModE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/17);  total (27/17)

AAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCGGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCTTTCGC  >  NC_000913/794281‑794533
                                                                                                                              |                                                                                                                              
aaaaaCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTgcg                                                                                                                   >  2:329415/1‑140 (MQ=255)
aaaaaCGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTgcg                                                                                                                   >  1:500419/1‑140 (MQ=255)
        gagaTCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCgccgccgcc                                                                                                           >  2:694132/1‑140 (MQ=255)
              gcgGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCttt                                                                                                     <  1:594748/140‑1 (MQ=255)
                     gcagGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCt                                                                                              >  1:610816/1‑140 (MQ=255)
                              tCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTgcgcgc                                                                                     <  1:329415/140‑1 (MQ=255)
                               cAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCt                                                                                    <  1:109974/140‑1 (MQ=255)
                                  cGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGa                                                                                 <  2:500419/140‑1 (MQ=255)
                                   ggCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGAc                                                                                <  1:988856/140‑1 (MQ=255)
                                   ggCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGAc                                                                                <  1:956706/140‑1 (MQ=255)
                                   ggCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGAc                                                                                >  1:877050/1‑140 (MQ=255)
                                       gggCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGa                                                                            >  2:158925/1‑140 (MQ=255)
                                       gggCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGa                                                                            <  1:761744/140‑1 (MQ=255)
                                          cGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAatt                                                                         >  2:379499/1‑139 (MQ=255)
                                            tCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATg                                                                       <  2:610816/140‑1 (MQ=255)
                                                   cACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACtt                                                                <  1:945885/140‑1 (MQ=255)
                                                     cTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTaa                                                              <  1:761163/140‑1 (MQ=255)
                                                         acacATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGa                                                          >  2:504853/1‑140 (MQ=255)
                                                          cacaTCAAAGGCTTTTTGCTGGATGTGCGCCAGAAAGTCATAGAGCTGAATCAGTCTCTGACCAAAGCTGGTCACTACTCCGCCGCAGCCACCTTTACCCCCTTTTGCGCGCTCGACCAGAATATGCACACTTAACTGAt                                                         >  2:547454/1‑140 (MQ=255)
                                                            caTCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTc                                                       >  1:211314/1‑140 (MQ=255)
                                                             aTCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGCTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCa                                                      >  1:640063/1‑140 (MQ=255)
                                                              tCAAAGGCTTTTTGCTGGATTTGCGCCAGGAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCAt                                                     >  1:204109/1‑140 (MQ=255)
                                                               cAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGTTCACTTAACTGATTCAtc                                                    >  1:477169/1‑140 (MQ=255)
                                                               cAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCAtc                                                    >  1:541181/1‑140 (MQ=255)
                                                                aaaGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCAtct                                                   >  2:605821/1‑140 (MQ=255)
                                                                    gCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGtt                                               >  2:250398/1‑140 (MQ=255)
                                                                       ttttGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAAt                                            >  1:418319/1‑140 (MQ=255)
                                                                         ttGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATgg                                          >  2:461693/1‑140 (MQ=255)
                                                                              ggATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATcc                                     >  1:853965/1‑140 (MQ=255)
                                                                                aTTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCa                                   <  2:877050/140‑1 (MQ=255)
                                                                                  ttGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTTCCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAgg                                 <  2:640063/140‑1 (MQ=255)
                                                                                   tGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGgc                                <  1:545870/140‑1 (MQ=255)
                                                                                    gcgcCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGgcg                               <  2:623033/140‑1 (MQ=255)
                                                                                         aGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCtttt                          <  2:853965/140‑1 (MQ=255)
                                                                                         aGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCtttt                          <  1:379499/140‑1 (MQ=255)
                                                                                                  tAGAGCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAAt                 >  1:348651/1‑140 (MQ=255)
                                                                                                     agCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATAcc              >  2:447286/1‑140 (MQ=255)
                                                                                                     agCTGAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATAcc              >  2:31424/1‑140 (MQ=255)
                                                                                                         gAATCAGTCGCTGACCATAGCTGGTCTGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCa          >  1:371338/1‑140 (MQ=255)
                                                                                                         gAATCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCCGTTGCGCGCTCGACCAGAATATGCTCACTTTACTGATTCATCTCGTTAATGGCATCCCACGCGCTTTTATAGCTAATTCCGCCa          >  2:634534/1‑140 (MQ=255)
                                                                                                         gAATCAGTCGCTGACCATAGCTGGTCAGAACTGCGCCGCCGCCACCTTTACTGCCTGTGGCGAGATCGACCAGAATATGCTCATTTAACTGATTCATCTCTTTAATGGCATCCCAGGCGCTTTTATAGCTAATGCCgtcc          >  2:95863/1‑137 (MQ=255)
                                                                                                          aaTCAGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCAt         >  1:985072/1‑140 (MQ=255)
                                                                                                              aGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCttt     <  2:985072/140‑1 (MQ=255)
                                                                                                              aGTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCttt     <  1:461693/140‑1 (MQ=255)
                                                                                                               gTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCTTTc    >  2:56965/1‑140 (MQ=255)
                                                                                                               gTCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCTTTc    >  2:284234/1‑140 (MQ=255)
                                                                                                                tCGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCTTTCg   <  1:605821/140‑1 (MQ=255)
                                                                                                                 cGCTGACCATAGCTGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCTTTCGc  >  2:526494/1‑140 (MQ=255)
                                                                                                                              |                                                                                                                              
AAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCGCTGACCATAGCGGGTCAGTACTGCGCCGCCGCCACCTTTACCGCCTGTTGCGCGCTCGACCAGAATATGCTCACTTAACTGATTCATCTCGTTAATGGCATCCCAGGCGCTTTTATAGCTAATACCGGCATCTTTCGC  >  NC_000913/794281‑794533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A