Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F48 I1 R1	70	151.6	5072932	98.7%	5006983	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	956,238	G→A	P132L (CCG→CTG)	ycaO ←	ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	956,238	0	G	A	100.0%	358.1 / NA	99	P132L (CCG→CTG)	ycaO	ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (52/47); total (52/47)

Only 100 of 101 total aligned reads displayed.

ACAGGTTACCAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCGGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCCCAGCCAGAAGTCCG  >  NC_000913/956101‑956373

aCAGGTTACCAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGa                                                                                                                                       >  1:1520182/1‑140 (MQ=255)

   ggTTACCAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCa                                                                                                                                    >  1:1623932/1‑140 (MQ=255)

        ccAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGTTTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATaaaa                                                                                                                               >  1:2526169/1‑140 (MQ=255)

        ccAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATaaaa                                                                                                                               >  1:937930/1‑140 (MQ=255)

         cAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAc                                                                                                                              >  1:21759/1‑140 (MQ=255)

            tGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAcgcg                                                                                                                           >  1:2530656/1‑140 (MQ=255)

              atatTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCGGGTCAGTTCATTCTCCAGATCATAAAAcgcgcg                                                                                                                         >  2:2174484/1‑140 (MQ=255)

              atatTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAcgcgct                                                                                                                         >  2:13454/1‑139 (MQ=255)

              atatTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAcgcgcg                                                                                                                         >  2:1536253/1‑140 (MQ=255)

              atatTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAcgcgcg                                                                                                                         >  1:2178133/1‑140 (MQ=255)

              atatTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAcgcgcg                                                                                                                         <  2:1829478/140‑1 (MQ=255)

              atatTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAAcgcgcg                                                                                                                         >  2:1195433/1‑140 (MQ=255)

                  tCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCTGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGa                                                                                                                     >  1:1879014/1‑140 (MQ=255)

                  tCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGa                                                                                                                     >  2:1218434/1‑140 (MQ=255)

                  tCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGa                                                                                                                     >  1:150912/1‑140 (MQ=255)

                  tCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGa                                                                                                                     <  1:221288/140‑1 (MQ=255)

                   cATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGAc                                                                                                                    <  1:2410151/140‑1 (MQ=255)

                      cGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGt                                                                                                                 >  1:1515096/1‑140 (MQ=255)

                       ggAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTc                                                                                                                <  1:223223/140‑1 (MQ=255)

                        gAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCa                                                                                                               <  2:545041/140‑1 (MQ=255)

                          atatAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTTATc                                                                                                             <  2:2077126/140‑1 (MQ=255)

                           aataAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCg                                                                                                            <  2:975146/139‑1 (MQ=255)

                             taAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCGGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGag                                                                                                          <  1:2174484/140‑1 (MQ=255)

                             taAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGag                                                                                                          <  1:1195433/140‑1 (MQ=255)

                               aaCGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGagca                                                                                                        <  2:21759/140‑1 (MQ=255)

                                aCGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGagcag                                                                                                       >  1:185852/1‑140 (MQ=255)

                                   gTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGccc                                                                                                    >  1:988819/1‑140 (MQ=255)

                                   gTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGccc                                                                                                    <  1:2100943/140‑1 (MQ=255)

                                   gTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGccc                                                                                                    >  1:1890400/1‑140 (MQ=255)

                                   gTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGccc                                                                                                    >  2:455760/1‑140 (MQ=255)

                                   gTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGccc                                                                                                    >  1:1191563/1‑140 (MQ=255)

                                   gTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGccc                                                                                                    >  1:1060277/1‑140 (MQ=255)

                                    tCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCt                                                                                                   >  2:2033268/1‑140 (MQ=255)

                                    tCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCt                                                                                                   >  2:58351/1‑140 (MQ=255)

                                    tCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCt                                                                                                   >  1:1314158/1‑140 (MQ=255)

                                      tGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCATTACTGGACTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTc                                                                                                 >  2:209511/1‑140 (MQ=255)

                                       gATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCt                                                                                                >  1:296164/1‑140 (MQ=255)

                                              ggACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGGCGGTCATCGAGCAGCCCTTCTGGCACAt                                                                                         >  1:190676/1‑140 (MQ=255)

                                               gACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATc                                                                                        <  1:455760/140‑1 (MQ=255)

                                                 cTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGt                                                                                      <  2:1670256/140‑1 (MQ=255)

                                                       cgTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCAtttt                                                                                <  2:1060277/140‑1 (MQ=255)

                                                          aaaCGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCgg                                                                             <  2:937930/140‑1 (MQ=255)

                                                          aaaCGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCgg                                                                             >  1:1062641/1‑140 (MQ=255)

                                                          aaaCGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCgg                                                                             >  1:1950068/1‑140 (MQ=255)

                                                           aaCGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGt                                                                            <  1:679974/140‑1 (MQ=255)

                                                           aaCGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGt                                                                            <  1:83503/140‑1 (MQ=255)

                                                             cGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATGGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACAGTCATCGAGCAGCCCTTCTGGCACATCGACATTTTCGGTCa                                                                          <  2:2526169/140‑1 (MQ=255)

                                                              ggCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAg                                                                         >  2:1715874/1‑140 (MQ=255)

                                                                 aGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTgg                                                                      <  2:75846/140‑1 (MQ=255)

                                                                  ggCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTggg                                                                     >  2:468845/1‑140 (MQ=255)

                                                                        aaaTACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCAt                                                               <  1:957658/140‑1 (MQ=255)

                                                                             ccACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTc                                                          >  1:1574402/1‑140 (MQ=255)

                                                                              cACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCg                                                         <  1:862921/140‑1 (MQ=255)

                                                                              cACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCg                                                         <  2:1623932/140‑1 (MQ=255)

                                                                               ccGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGt                                                        <  1:1566931/139‑1 (MQ=255)

                                                                                cGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGtt                                                       >  1:259815/1‑140 (MQ=255)

                                                                                 gATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTg                                                      <  2:216415/140‑1 (MQ=255)

                                                                                  aTCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTgg                                                     >  1:1323071/1‑140 (MQ=255)

                                                                                    cTTCGTTGCCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGa                                                   >  1:2343641/1‑140 (MQ=255)

                                                                                    cTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGa                                                   <  1:451191/140‑1 (MQ=255)

                                                                                    cTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGa                                                   <  1:1715874/140‑1 (MQ=255)

                                                                                    cTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGa                                                   <  1:594228/140‑1 (MQ=255)

                                                                                     ttCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGAt                                                  >  2:73702/1‑140 (MQ=255)

                                                                                      tCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATa                                                 >  2:1654794/1‑140 (MQ=255)

                                                                                        gTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAAt                                               >  2:2092889/1‑140 (MQ=255)

                                                                                           aCCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCa                                            <  2:682843/140‑1 (MQ=255)

                                                                                           aCCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCa                                            <  2:1062641/140‑1 (MQ=255)

                                                                                           aCCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGAGATAATGCa                                            <  2:1630811/140‑1 (MQ=255)

                                                                                             cGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACg                                          >  2:8637/1‑140 (MQ=255)

                                                                                             cGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACg                                          >  2:787166/1‑140 (MQ=255)

                                                                                             cGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTCTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACg                                          >  1:1429287/1‑140 (MQ=255)

                                                                                              ggATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGa                                         <  2:1520182/140‑1 (MQ=255)

                                                                                               gATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGaa                                        >  1:318021/1‑140 (MQ=255)

                                                                                                 ttGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACg                                      <  2:1596467/140‑1 (MQ=255)

                                                                                                 ttGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACg                                      <  1:1819526/140‑1 (MQ=255)

                                                                                                  tGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACgg                                     <  2:150912/140‑1 (MQ=255)

                                                                                                          aaTCAGCATGCTGCCGGTCAGTTCATTCTCCAGATTATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTgg                             >  2:764468/1‑140 (MQ=255)

                                                                                                          aaTCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTgt                             >  2:306169/1‑139 (MQ=255)

                                                                                                           aTCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGc                            >  1:2238444/1‑140 (MQ=255)

                                                                                                            tCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCg                           <  2:259815/140‑1 (MQ=255)

                                                                                                            tCAGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCg                           >  1:187101/1‑140 (MQ=255)

                                                                                                              aGCATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGAt                         >  2:1656231/1‑140 (MQ=255)

                                                                                                                cATGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATgg                       >  1:1579418/1‑140 (MQ=255)

                                                                                                                 aTGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGa                          >  2:915661/1‑136 (MQ=255)

                                                                                                                 aTGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGa                          <  1:915661/136‑1 (MQ=255)

                                                                                                                 aTGCTGCCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGt                      <  1:58351/140‑1 (MQ=255)

                                                                                                                     tgcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCg                  >  1:2280177/1‑140 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 <  2:2238444/140‑1 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 <  2:206113/140‑1 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 <  1:1658638/140‑1 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 <  1:1656231/140‑1 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 >  1:1486167/1‑140 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 <  1:1529466/140‑1 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGc                 <  2:403113/140‑1 (MQ=255)

                                                                                                                      gcCGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGAACGTTGGCGATGGTTTCGc                 <  2:1574402/140‑1 (MQ=255)

                                                                                                                       ccGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGcc                <  2:1314158/140‑1 (MQ=255)

                                                                                                                        cGGTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGccc               <  1:73702/140‑1 (MQ=255)

                                                                                                                          gTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCccag             >  2:315115/1‑140 (MQ=255)

                                                                                                                          gTCAGTTCATTCTCCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCccag             >  1:2533338/1‑140 (MQ=255)

                                                                                                                                     ctcCAGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCCCAGCCAGAAGTCCg  <  1:468845/140‑1 (MQ=255)

ACAGGTTACCAATGATATTCATCGGAATATAAACGGTCTGATTGTCGGACTGACGCGTAAACGGCAGGCCGCAAATACCACGATCTTCGTTACCGGATTGTAGGTCAATCAGCATGCTGCCGGTCAGTTCATTCTCCGGATCATAAAACGCGCGCAGACGGTCATCGAGCAGCCCTTCTGGCACATCGTCATTTTCGGTCAGTGGGAACCATTTTTCGTTGGGATAATGCACGAACGGACCGTTGGCGATGGTTTCGCCCAGCCAGAAGTCCG  >  NC_000913/956101‑956373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A