Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F53 I0 R3 198 62.2 2365906 95.5% 2259440 127.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,642 C→G 100% P152P (CCC→CCG)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,6420CG100.0% 183.4 / NA 54P152P (CCC→CCG) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (34/20);  total (34/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCC  >  CP006881/3723507‑3723777
                                                                                                                                       |                                                                                                                                       
gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCt                                                                                                                                      >  3:193903/1‑139 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGCCGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGCGccgcccgcgt                                                                                                                                     >  4:18177/1‑137 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTTATTCCTTTTTGATTCATTTGGCAGAGCACAAAATTGAGccgccggcgt                                                                                                                                     >  1:273137/1‑137 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                                                                                                     >  3:42736/1‑139 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                                                                                                     >  7:61797/1‑139 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCATTTTTGATTCCTTTTGCAGAGAACAAAATGGAGCCGACGGCtt                                                                                                                                     >  6:32183/1‑139 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTAATTCCTTTTTTATTAATTTGGCAGAGCACAAAATTGATCCGCCGGCtt                                                                                                                                     >  2:289687/1‑139 (MQ=255)
 tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCAACCTGATCAGCGCGACGGGACTGTTTGAGGAGCCTTCTGGTCATTCATTTTTGAGTCATTTGTCAGAGCACAAAATGGACCAGCCGGCt                                                                                                                                      >  5:87250/1‑138 (MQ=255)
   gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                                                                                                   >  2:169034/1‑139 (MQ=255)
   gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                                                                                                   >  5:100340/1‑139 (MQ=255)
   gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                                                                                                   >  2:86838/1‑139 (MQ=255)
   gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTATGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGGAGAGCACAAAATGGAGCCGCCGGCTTcc                                                                                                                                   >  1:39241/1‑139 (MQ=255)
    aTGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                                                                                                                                  <  8:21525/139‑1 (MQ=255)
     tGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                                                                                                                                  >  7:42011/1‑138 (MQ=255)
        cAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaatt                                                                                                                              >  7:26948/1‑137 (MQ=255)
        cAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaatt                                                                                                                              >  3:24481/1‑137 (MQ=255)
               tGCCGGCAATTTACTCGGCGCCATGCGGGTGGCCATCCTGAGCCGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCCGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                                                                                        <  2:87996/138‑1 (MQ=255)
               tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                                                                                        <  3:276504/138‑1 (MQ=255)
                             tCGGCGCCATTCTGTTTGCCATCGTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtgtg                                                                                                         <  6:150026/139‑1 (MQ=255)
                               ggCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCa                                                                                                       <  1:86838/139‑1 (MQ=255)
                                gcgcCATTGTGTTTGCCATCCTTATCAGCGCGACGGGTCTTTTTGAGGAGCGTTCTGTTCATTCCTTTTTTATTCATTTGGAAGAGCACAAAAGGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                                                                                                      <  2:39241/139‑1 (MQ=255)
                                gcgcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                                                                                                      <  8:42011/139‑1 (MQ=255)
                                gcgcCATACTGTTTGCACTCCTTATCACCGCGAGGGGACTTTTTGAGGCGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                                                                                                      <  4:256644/139‑1 (MQ=255)
                                  gcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt                                                                                                    <  8:61797/139‑1 (MQ=255)
                                           tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTTGCAGAGCACAAAATGGAGCCGCCGGCGTCCGAATTTTTTTTAAGAGGTATTGTGTGGAATTTGCgtgtgt                                                                                           >  1:137626/1‑139 (MQ=255)
                                               ccATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCATAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc                                                                                        <  1:169034/138‑1 (MQ=255)
                                                   ccTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGcc                                                                                   >  2:60693/2‑139 (MQ=255)
                                                     tGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGCATGCTTTGCAATTTGCTTGTGTGCCTCGCGtt                                                                                 >  2:275662/1‑139 (MQ=255)
                                                       tcccgcgcGACGGGAATTTTTGAGTAGCCTTCTGTTAATTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt                                                                               <  2:137626/135‑1 (MQ=255)
                                                       aTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt                                                                               >  8:220273/1‑139 (MQ=255)
                                                          accgcgACGGGACTTTTTGAGGAGCCTTCTGTTAATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCttttt                                                                              <  5:43266/135‑1 (MQ=255)
                                                            cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTTTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCGTTTTCAtt                                                                          >  3:1532/1‑139 (MQ=255)
                                                            cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTACGAATTGTTTTTCAGAGGAATTCTTTGCAATTTGCTTGTGTTCCTCGCCTTTTTGAtt                                                                          >  3:43629/1‑139 (MQ=255)
                                                              cgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                                                                                        <  5:290127/91‑1 (MQ=255)
                                                              cgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                                                                                        >  6:290127/1‑91 (MQ=255)
                                                                    gacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGtc                                                                  <  2:367/137‑1 (MQ=255)
                                                                              ggagcCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaaggg                                                        >  3:292577/1‑139 (MQ=255)
                                                                                 gccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggg                                                       >  8:281097/3‑137 (MQ=255)
                                                                                       tGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                               <  4:42736/139‑1 (MQ=255)
                                                                                         ttCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa                                               <  1:8030/137‑1 (MQ=255)
                                                                                            aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGct                                          >  6:90550/1‑139 (MQ=255)
                                                                                            aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCACCTTTTTCATTCCAATGTCTCTCAaagggga                                                      <  5:147377/127‑1 (MQ=255)
                                                                                            aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATAGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCCTTCCAATGTCTCTCAAAGGGGAAGGCGCAAAGct                                          >  2:33988/1‑139 (MQ=255)
                                                                                            aTTCCTTTTTGATTCAATTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGt                                                                   <  7:37331/114‑1 (MQ=255)
                                                                                            aTTCCTTTTTGATTCAATTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGt                                                                   >  8:37331/1‑114 (MQ=255)
                                                                                            aTTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCACCTTTTTCATTCCAATGTCTCTCAaagggga                                                      >  6:147377/1‑127 (MQ=255)
                                                                                              tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt                                        >  7:46622/1‑137 (MQ=255)
                                                                                              tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt                                        >  1:7553/1‑137 (MQ=255)
                                                                                                cTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCtttt                                       <  4:292577/138‑1 (MQ=255)
                                                                                                   tttGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTGTCGCAAAGGGGAAGGAGCAAACCTTTTTAc                                    >  8:107783/1‑138 (MQ=255)
                                                                                                   tttGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCATCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAACCTTTTTAc                                    >  7:201655/1‑138 (MQ=255)
                                                                                                        ttCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCatgat                              >  6:93190/1‑139 (MQ=255)
                                                                                                          cATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGc                            >  6:67944/1‑139 (MQ=255)
                                                                                                                    agCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGtttt                  <  8:46622/139‑1 (MQ=255)
                                                                                                                         aaaaTGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCATCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGct             >  2:201700/1‑139 (MQ=255)
                                                                                                                                aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTTGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAt      >  4:22014/1‑139 (MQ=255)
                                                                                                                                    gccggcTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTcc  >  3:198939/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCC  >  CP006881/3723507‑3723777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A