Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,020	G→A	A302A (GCC→GCT)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,020	0	G	A	100.0%	139.5 / NA	46	A302A (GCC→GCT)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/27); total (19/27)

TGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGA  >  CP006881/2173884‑2174152

tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                                    >  1:112670/1‑139 (MQ=255)

tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                                    >  8:2406/1‑139 (MQ=255)

tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                                    >  1:331649/1‑139 (MQ=255)

tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                                    >  6:66793/1‑139 (MQ=255)

 gATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                                   <  5:253246/139‑1 (MQ=255)

  atttCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTGAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                                   <  4:422298/135‑1 (MQ=255)

  atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATGGAGTTTTTCGTTTTGCCGATGCTGCGTTATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtg                                                                                                                                  <  5:140245/139‑2 (MQ=255)

  atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc                                                                                                                                  <  3:422123/139‑2 (MQ=255)

  atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                                   <  7:132528/138‑1 (MQ=255)

  atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                                   <  4:66477/138‑1 (MQ=255)

  atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAAAGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc                                                                                                                                  <  6:204147/139‑2 (MQ=255)

       aaGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTa                                                                                                                              <  1:18396/138‑1 (MQ=255)

               tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                                                                                                                                <  8:209914/128‑1 (MQ=255)

               tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                                                                                                                                >  7:209914/1‑128 (MQ=255)

                aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                     <  2:112670/138‑1 (MQ=255)

                aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                     <  2:415330/138‑1 (MQ=255)

                      tttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                               >  5:393025/1‑138 (MQ=255)

                       ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                               >  3:271622/1‑137 (MQ=255)

                       ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                               >  5:259403/1‑137 (MQ=255)

                       ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCa                                                                                                              <  5:66793/138‑1 (MQ=255)

                           tCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGct                                                                                                         <  4:271622/139‑1 (MQ=255)

                                             cgtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcgg                                                                                        >  3:94325/6‑138 (MQ=255)

                                              gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                      <  8:308515/135‑3 (MQ=255)

                                              gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                      <  7:293885/135‑3 (MQ=255)

                                              gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                      <  5:226415/135‑3 (MQ=255)

                                              gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                      <  3:266262/135‑3 (MQ=255)

                                                  tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa                                                                                                                            <  4:83430/97‑1 (MQ=255)

                                                  tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa                                                                                                                            >  3:83430/1‑97 (MQ=255)

                                                      tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt                                                                              >  5:350233/1‑139 (MQ=255)

                                                      tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt                                                                              >  6:290236/1‑139 (MQ=255)

                                                       gTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGAGGTTTTTTCCCAGCGCTGTACAGTGTCATTGCGTCACCGCTCTGGCCAACCGTATCGGCAAGGTGAAtg                                                                             >  1:215743/1‑138 (MQ=255)

                                                                   cttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgat                                                                  <  7:2406/134‑1 (MQ=255)

                                                                    ttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgatca                                                                <  6:259403/136‑1 (MQ=255)

                                                                                        ttGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCa                                                                                                  <  4:412150/85‑1 (MQ=255)

                                                                                        ttGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCa                                                                                                  >  3:412150/1‑85 (MQ=255)

                                                                                         tGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                     <  6:1304/65‑1 (MQ=255)

                                                                                         tGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                     >  5:1304/1‑65 (MQ=255)

                                                                                              aaGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTc                                      <  2:331649/139‑1 (MQ=255)

                                                                                                    cGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGaa                                 >  1:359579/1‑138 (MQ=255)

                                                                                                              gCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGgcgcgt                      >  1:42345/1‑137 (MQ=255)

                                                                                                                  cTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCttt                  <  2:42345/139‑1 (MQ=255)

                                                                                                                   tGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTc                 >  3:440333/1‑139 (MQ=255)

                                                                                                                            tgcttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtg        >  4:305824/4‑138 (MQ=255)

                                                                                                                              cttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc      <  3:305824/138‑4 (MQ=255)

                                                                                                                              cgattttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc      <  8:197962/136‑4 (MQ=255)

                                                                                                                                   ttcccAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATAGTCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAgg  <  4:179756/136‑2 (MQ=255)

TGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGA  >  CP006881/2173884‑2174152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A