Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,183,400	G→A	F43F (TTC→TTT)	U712_10935 ←	Uncharacterized protein ypiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,183,400	0	G	A	99.3%	428.0 / NA	135	F43F (TTC→TTT)	U712_10935	Uncharacterized protein ypiF
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (72/62); minor base T (0/1); total (72/63)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CTGATATAAGCTCTTCCCGCAAATCCCTAAGTCCTTGAACGGTTATTTCATTTCTATCAACATATGTATATGGGGGCAGTAAATATACCCGTCCCTTTAACTGCCTTTCCAGTTCTTCTGACAGAAGCTGCGTGAATTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATCAGTGATTCTCCACCTCATCGTTCTC  >  CP006881/2183267‑2183535

ccgttatcAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTaaact                                                                                                                                     <  8:318327/134‑3 (MQ=255)

ccgttatcAGCTCTTCCCGCAAATCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTaaact                                                                                                                                     <  3:183574/134‑3 (MQ=255)

                 cGCAAATCCTTCAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCa                                                                                                                   <  4:165665/139‑1 (MQ=255)

                    aaaTCCTTAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCAttt                                                                                                                >  5:14161/1‑139 (MQ=255)

                         cttAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGACGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTaaaa                                                                                                           >  4:96669/3‑139 (MQ=255)

                         cttAAGCCCTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTaaaa                                                                                                           >  4:102907/3‑139 (MQ=255)

                             agcccTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATgg                                                                                                        <  3:408721/135‑1 (MQ=255)

                                 cTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  3:198141/139‑1 (MQ=255)

                                 cTTGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  8:10656/139‑1 (MQ=255)

                                  ttGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCATGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  4:33943/1‑138 (MQ=255)

                                  ttGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  5:137340/1‑138 (MQ=255)

                                  ttGAACGGTTATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  3:316471/1‑138 (MQ=255)

                                   tGAACGGTTATTTCATTTCTATCAACAAATGTATAACGAGGAAGTAAATATACACGTCCCTTTAACTGGCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTACCCTGTCTCCGCCGCCAATTTATAATGAttatta                                                                                                  >  1:196440/1‑138 (MQ=255)

                                          ttATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTAt                                                                                          >  3:322663/1‑139 (MQ=255)

                                          ttATTTCATTTCTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTAt                                                                                          >  2:91640/1‑139 (MQ=255)

                                          ttATTTCATTTCAATCAACATATGTATAAGGCGGAAGTAAATATACACGTCCCTTTAACTGACTTTCAAGATATACTCAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATAGTTATTAACACTTaa                                                                                          >  7:369566/1‑138 (MQ=255)

                                            atttcatttcTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGt                                                                                        >  3:234846/1‑139 (MQ=255)

                                            atttcatttcTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGt                                                                                        >  3:228548/1‑139 (MQ=255)

                                                 atttcTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGttaatt                                                                                   <  8:273481/139‑1 (MQ=255)

                                                    tcTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTtaattaat                                                                                >  5:404885/1‑139 (MQ=255)

                                                    tcTATCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTtaattaat                                                                                >  7:260739/1‑139 (MQ=255)

                                                       aTCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTATCTGCCTTTCAAGTTCTTCTGCGAGCAGCTGCGTAAACTCGCCGTTCACCGCCGCCATGTTGAAAAGGTTATTATACCTTATGTtaatttatttt                                                                             >  6:285631/1‑136 (MQ=255)

                                                        tCAACATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTa                                                                            >  8:50637/1‑139 (MQ=255)

                                                            cATATGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGa                                                                         >  3:443902/1‑138 (MQ=255)

                                                              tatGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      <  4:228548/139‑1 (MQ=255)

                                                              tatGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      <  4:234846/139‑1 (MQ=255)

                                                              tatGTATAAGGCGGAAGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      <  4:322663/139‑1 (MQ=255)

                                                                       ggcggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGTTATGACAGCGGTATCaa                                                             >  1:4872/7‑139 (MQ=255)

                                                                       ggcggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                >  1:120054/7‑136 (MQ=255)

                                                                       ggcggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                <  2:120054/130‑1 (MQ=255)

                                                                       ggcggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCaa                                                             >  7:152047/7‑139 (MQ=255)

                                                                       ggcggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCaa                                                             >  3:45343/7‑139 (MQ=255)

                                                                         cggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                >  6:175098/5‑134 (MQ=255)

                                                                         cggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                <  5:175098/130‑1 (MQ=255)

                                                                          ggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  3:394103/4‑98 (MQ=255)

                                                                          ggaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  4:394103/95‑1 (MQ=255)

                                                                           gaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  8:185852/95‑1 (MQ=255)

                                                                           gaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  7:185852/3‑97 (MQ=255)

                                                                           gaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGt                                                          >  3:311269/3‑138 (MQ=255)

                                                                           gaaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGt                                                          >  4:97076/3‑138 (MQ=255)

                                                                            aaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGATAGAAGCTGCGGAAACTCGCCTTTCTCCTCCGCCATTTTAAAAAGGTTATTAACCCTTATGTTTATTAATGGTATGACAGCGGTATCAATGTaa                                                        >  1:222794/2‑139 (MQ=255)

                                                                            aaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      >  5:184837/2‑125 (MQ=255)

                                                                            aaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      <  6:184837/124‑1 (MQ=255)

                                                                            aaGTAAATATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                >  4:198624/2‑131 (MQ=255)

                                                                                 aaTATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAATCTGAGTTAACTCGCCTTTCTACGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGAGGTATCAATGTAATCCtt                                                   <  7:171581/139‑1 (MQ=255)

                                                                                 aaTATACCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCtt                                                   <  8:152047/139‑1 (MQ=255)

                                                                                     taCCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCt                                               <  1:52079/139‑1 (MQ=255)

                                                                                      aCCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  6:347843/1‑86 (MQ=255)

                                                                                      aCCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  5:347843/86‑1 (MQ=255)

                                                                                      aCCCGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCAGTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCtt                                              >  6:360451/1‑139 (MQ=255)

                                                                                       cccGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCtt                                              <  7:50637/138‑1 (MQ=255)

                                                                                       cccGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCtt                                              <  7:57449/138‑1 (MQ=255)

                                                                                         cGTCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATg                                                                                                         <  4:428824/77‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAAt                                                                                                          >  6:226329/1‑74 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAAt                                                                                                          <  5:311086/74‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAAt                                                                                                          <  5:226329/74‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  5:244157/1‑81 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  6:152223/81‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  5:238190/1‑81 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  6:244157/81‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGttaatt                                                                                   >  4:235824/1‑97 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      <  4:335727/110‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAg                                                                      >  3:335727/1‑110 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTa                                                                 >  4:43887/1‑115 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTa                                                                 <  3:43887/115‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGt                                                          <  1:72305/122‑1 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGt                                                          >  2:72305/1‑122 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTaaa                                         >  5:415155/1‑139 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTaaa                                         >  6:367487/1‑139 (MQ=255)

                                                                                           tCCCTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTaa                                          >  3:146860/1‑138 (MQ=255)

                                                                                           tCCCTTTAACTGCCATTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGttaatt                                                                                   <  3:235824/97‑1 (MQ=255)

                                                                                             ccTTTAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCATTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATa                                       >  8:414996/1‑139 (MQ=255)

                                                                                               tttAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTTATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGt                                     >  4:213147/1‑139 (MQ=255)

                                                                                               tttAACTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGt                                     >  4:359421/1‑139 (MQ=255)

                                                                                                  aaCTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCt                                  >  1:197688/1‑139 (MQ=255)

                                                                                                  aaCTGCCTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCt                                  >  2:171432/1‑139 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTaaaa                                                                                                           >  6:20932/1‑61 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  2:123271/69‑1 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  1:123271/1‑69 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATg                                                           <  7:146544/109‑1 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATg                                                           >  8:146544/1‑109 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCtt                                              >  1:400878/1‑122 (MQ=255)

                                                                                                       ccTTTCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCtt                                              <  2:400878/122‑1 (MQ=255)

                                                                                                        cTTTCTAGTTCTTCTGAGAGTTTCTGCGTAAACTCGCCTTTCTCAGCCGGCTTTTTAAAATTGTTATTTTGCCTTATGTTAATTATTGGTATTACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTGGTTGGCAt                            <  8:369566/139‑1 (MQ=255)

                                                                                                         tttCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAAt                                                                                                          <  6:250623/60‑1 (MQ=255)

                                                                                                         tttCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  5:188327/67‑1 (MQ=255)

                                                                                                         tttCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTa                                                                 >  7:364932/1‑101 (MQ=255)

                                                                                                         tttCAAGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTa                                                                 <  8:364932/101‑1 (MQ=255)

                                                                                                           tcaaGTTCTTCTGAGAGAAGCTGCGTAAACTAGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATc                           <  1:374840/134‑1 (MQ=255)

                                                                                                             aaGTTCTTCTGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATCAGt                        <  1:106618/137‑1 (MQ=255)

                                                                                                                 tcttctGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   >  7:376001/1‑59 (MQ=255)

                                                                                                                 tcttctGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGttatt                                                                                                   <  8:376001/59‑1 (MQ=255)

                                                                                                                  cttctGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                <  6:101607/93‑1 (MQ=255)

                                                                                                                  cttctGAGAGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTAt                                                                >  5:101607/1‑93 (MQ=255)

                                                                                                                        agaGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATCAGTGATTCTCCa               <  2:272012/134‑1 (MQ=255)

                                                                                                                          aGAAGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGAATCTGTGATTCTCCACCTCa          >  4:418370/1‑139 (MQ=255)

                                                                                                                          aGAAGCTGCGTAAACTCGCCGTTCTCCGCCGCCATTTTAAAATGGGTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGGCCTTGGCATCAGTGATTCTCCACCTCa          <  1:404460/139‑1 (MQ=255)

                                                                                                                            aaGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGttaattaa                                                                                 >  3:357923/1‑66 (MQ=255)

                                                                                                                             aGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATCAg                         >  4:367637/1‑121 (MQ=255)

                                                                                                                             aGCTGCGTAAACTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATCAg                         <  3:367637/121‑1 (MQ=255)

CTGATATAAGCTCTTCCCGCAAATCCCTAAGTCCTTGAACGGTTATTTCATTTCTATCAACATATGTATATGGGGGCAGTAAATATACCCGTCCCTTTAACTGCCTTTCCAGTTCTTCTGACAGAAGCTGCGTGAATTCGCCTTTCTCCGCCGCCATTTTAAAATGGTTATTAACCCTTATGTTAATTAATGGTATGACAGCGGTATCAATGTAATCCTTAGCTTGTAAATAGTCCTTGGCATCAGTGATTCTCCACCTCATCGTTCTC  >  CP006881/2183267‑2183535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A