Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,658	T→C	A160A (GCA→GCG)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,658	0	T	C	98.9%	280.7 / NA	87	A160A (GCA→GCG)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (47/39); minor base A (1/0); total (48/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAGCGCTCAAGCACATTTCTCATATCACGGTGATTATATTTAATCGCTC  >  CP006881/2197522‑2197788

tttcatccATCTTTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATATGCAACACGTGGCCCGc                                                                                                                                  <  1:424370/133‑1 (MQ=255)

tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                                                  <  6:357860/139‑1 (MQ=255)

tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                                                  <  5:251040/139‑1 (MQ=255)

     gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCt                                                                                                                             <  1:194088/139‑1 (MQ=255)

     gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGGTAAACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCt                                                                                                                             >  6:212272/1‑139 (MQ=255)

                         ctcttcATCGAAGCTTCTGACAGGTGACTCTTCCGTTACACGCTGCATGGCTTCAATTGCCTTATACTCTGTTTTTTCAGCTTTAACAGCACCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCagaaaga                                                                                                         <  4:202173/138‑1 (MQ=255)

                                   tAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAg                                                                                               >  4:364041/1‑139 (MQ=255)

                                    aGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGc                                                                                              >  7:429960/1‑139 (MQ=255)

                                    aGCATATGACAGGATACTCTTCATTAACAAGCAGCAAGTCTTCAAATGACGTATACTCTCTATTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAATGCCAAGCTCAGAAAGAATCtttt                                                                                                  >  6:90409/1‑135 (MQ=255)

                                        tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta                                                                                            >  5:279050/1‑135 (MQ=255)

                                        tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta                                                                                            >  3:56044/1‑135 (MQ=255)

                                           gACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCAAATTTGCAACACGTGGCCAGCCACCTTAATGACAAGCTAAGAAAGAATCTTTTTAGCTACatcc                                                                                       >  8:22065/1‑136 (MQ=255)

                                           gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                                                                                       >  8:74225/1‑138 (MQ=255)

                                           gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                                                                                       >  8:15602/1‑138 (MQ=255)

                                           gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                                                                                       >  5:439260/1‑138 (MQ=255)

                                             caggAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACGTATACTCTGTATATTCAGCAATAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcccc                                                                                     <  3:142479/137‑1 (MQ=255)

                                                gagaCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCGTAATGCCAAGCTCAGAAAGAATCTTGTTAGCTACAGCCCCTgc                                                                                  >  8:169235/1‑139 (MQ=255)

                                                   aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc                                                                               >  7:180844/1‑139 (MQ=255)

                                                   aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGAAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc                                                                               >  4:134128/1‑139 (MQ=255)

                                                       ttccGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCAct                                                                           >  1:181724/2‑139 (MQ=255)

                                                         ccGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCAc                                                                            <  3:364041/136‑1 (MQ=255)

                                                          cGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCActct                                                                         <  7:74225/138‑1 (MQ=255)

                                                                 cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                                                  <  1:439951/74‑1 (MQ=255)

                                                                 cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                                                  >  2:439951/1‑74 (MQ=255)

                                                                 cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAgttg                                                                 >  3:184275/1‑139 (MQ=255)

                                                                 cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAgttg                                                                 >  6:218238/1‑139 (MQ=255)

                                                                    tGCAAGTCTTCAATTGACGTTTACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAAGGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCAACGCTGCCAgttgtt                                                               >  8:278346/1‑138 (MQ=255)

                                                                    tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCCCTCTGACAGTTGttt                                                              >  4:144643/1‑139 (MQ=255)

                                                                    tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGttt                                                              >  2:49567/1‑139 (MQ=255)

                                                                    tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGttt                                                              >  2:28854/1‑139 (MQ=255)

                                                                      caagTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTcc                                                            >  2:80139/4‑139 (MQ=255)

                                                                            ttcaATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCt                                                      >  2:321058/2‑139 (MQ=255)

                                                                            ttcaATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCt                                                      >  7:268256/2‑139 (MQ=255)

                                                                              caATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTa                                                                                                                           >  6:258670/1‑68 (MQ=255)

                                                                              caATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTa                                                                                                                           <  5:258670/68‑1 (MQ=255)

                                                                                   gACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAagaa                                                <  1:28854/138‑2 (MQ=255)

                                                                                         tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCt                                                                                                                 >  6:59307/4‑67 (MQ=255)

                                                                                         tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCt                                                                                                                 <  5:59307/64‑1 (MQ=255)

                                                                                         tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAagaa                                                <  6:38429/129‑2 (MQ=255)

                                                                                         tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAagaa                                                >  5:38429/4‑131 (MQ=255)

                                                                                         tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCaa                                         >  1:200984/4‑139 (MQ=255)

                                                                                          actCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAg                                        <  2:181724/137‑1 (MQ=255)

                                                                                              tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCTAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGcaca                                    >  2:23008/1‑139 (MQ=255)

                                                                                              tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGcaca                                    >  5:444032/1‑139 (MQ=255)

                                                                                              tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGcaca                                    >  2:357595/1‑139 (MQ=255)

                                                                                              tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGca                                      <  8:3946/137‑1 (MQ=255)

                                                                                               gTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAt                                   >  4:41350/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  6:290697/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  8:156886/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  4:397749/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  6:140608/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  8:368578/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  7:403465/1‑139 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAt                                   >  2:319475/1‑138 (MQ=255)

                                                                                                ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCATGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt                                  >  5:249005/1‑139 (MQ=255)

                                                                                                        gCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAg                                        <  6:183961/125‑1 (MQ=255)

                                                                                                        gCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAg                                        >  5:183961/1‑125 (MQ=255)

                                                                                                        gCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCatat                          <  4:184275/139‑1 (MQ=255)

                                                                                                        gCTTTAACAGCGCCAATTTGCAACACGTGGACCGCCACCTTAAAGCCAAGCTCAGAAAGAATCTTATTAGCTAAAGCCCCTGCCGCCACTCTGACATTAGTTTACCTTGCTGAAGAACACTCAAGCACAGTTAACata                           >  6:354610/1‑138 (MQ=255)

                                                                                                            tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc                       >  1:147105/1‑138 (MQ=255)

                                                                                                            tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc                       <  8:403465/138‑1 (MQ=255)

                                                                                                            tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc                       >  3:222091/1‑138 (MQ=255)

                                                                                                            tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc                       <  8:268256/138‑1 (MQ=255)

                                                                                                             aaCAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACg                      <  5:140608/138‑1 (MQ=255)

                                                                                                             aaCAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACg                      <  3:41350/138‑1 (MQ=255)

                                                                                                                agcgcCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGa                  <  2:423868/138‑1 (MQ=255)

                                                                                                                agcgcCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGa                  <  6:172034/138‑1 (MQ=255)

                                                                                                                   gcCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTccc                                                           >  8:119948/1‑95 (MQ=255)

                                                                                                                   gcCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTccc                                                           <  7:119948/95‑1 (MQ=255)

                                                                                                                   gcCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATta               >  6:271173/1‑139 (MQ=255)

                                                                                                                   gcCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATta               >  1:236038/1‑139 (MQ=255)

                                                                                                                     cAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATtata             <  8:429960/139‑1 (MQ=255)

                                                                                                                     cAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATtata             <  2:236038/139‑1 (MQ=255)

                                                                                                                       aTTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATAtt           <  4:56044/139‑1 (MQ=255)

                                                                                                                       aTTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATAtt           <  6:279050/139‑1 (MQ=255)

                                                                                                                            cAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAAt       <  4:81987/138‑1 (MQ=255)

                                                                                                                            cAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTATTTAGCTACAGCCCCTGCCGCCACTCTGTCAGTTGGTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAAtt      >  3:360783/1‑138 (MQ=255)

                                                                                                                             aacaCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAAttg     <  5:218238/136‑3 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAATGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  5:354610/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTTCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  5:212272/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  1:357595/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  1:80139/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  5:271173/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  7:368578/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  6:439260/138‑2 (MQ=255)

                                                                                                                               caCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATATTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGca   <  7:169235/138‑2 (MQ=255)

                                                                                                                                aCGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGcac  <  1:319475/139‑3 (MQ=255)

TGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAGCGCTCAAGCACATTTCTCATATCACGGTGATTATATTTAATCGCTC  >  CP006881/2197522‑2197788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A