Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,419 A→C A188A (GCT→GCG)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,4190AC100.0% 181.2 / NA 57A188A (GCT→GCG) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/22);  total (35/22)

GCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCT  >  CP006881/2198284‑2198549
                                                                                                                                       |                                                                                                                                  
gctgcCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATTCAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGcc                                                                                                                                 <  7:112268/139‑1 (MQ=255)
 ctgcCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCa                                                                                                                                <  7:239346/139‑1 (MQ=255)
    ccGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAgc                                                                                                                              >  1:60363/1‑137 (MQ=255)
       aCAAAAAGAACTCCATTCTTCATCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAgcaaat                                                                                                                          >  6:114764/1‑134 (MQ=255)
         aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                        >  1:151721/1‑139 (MQ=255)
         aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                        >  7:137227/1‑139 (MQ=255)
         aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                        >  3:111693/1‑139 (MQ=255)
         aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                        >  2:442834/1‑139 (MQ=255)
         aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTAATAACGATCCGCCAGCAAATtg                                                                                                                        >  5:254012/1‑139 (MQ=255)
         aaaaAGAACTCCAATCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTACTTCGCGCTTTATGTAAAATAAATAAATACATAGAGGCACAAGATTAAGTAAAACTCTTGTTCAAAAAGATCCGCCAGCAAATtg                                                                                                                        >  7:2940/1‑139 (MQ=255)
                    cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACTCGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGa                                                                                                              <  8:123737/138‑1 (MQ=255)
                    cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGa                                                                                                              <  8:201869/138‑1 (MQ=255)
                            atCAAGCTATGTGCGATTTTTTAATACCGCGCTTCCTTGGCGCTTTCTGTTAAATAATTAATTTCATTGCGGCACAGGATTAAGGCTAAGTCTAGTTCTTAAAGATCCGCCAGAAAATTTTGATTATTGAATGtattat                                                                                                     >  2:345789/2‑139 (MQ=255)
                              cAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTc                                                                                                   >  8:254042/1‑139 (MQ=255)
                                     tgtgCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGa                                                                                            >  3:71555/1‑139 (MQ=255)
                                     tgtgCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGa                                                                                            >  5:19195/1‑139 (MQ=255)
                                             ttttAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGTCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCgtt                                                                                    >  6:68061/1‑137 (MQ=255)
                                             ttttAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCgtt                                                                                    >  8:306419/1‑137 (MQ=255)
                                                taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                  <  7:306419/138‑1 (MQ=255)
                                                taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                  <  1:442834/138‑1 (MQ=255)
                                                taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                  <  8:2940/138‑1 (MQ=255)
                                                taaatacaGCTCTTCCTTCGCGCCTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                  <  5:114764/138‑1 (MQ=255)
                                                taaatacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGGTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                  <  1:252732/138‑1 (MQ=255)
                                                  aatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGttttca                                                                                <  4:71555/138‑3 (MQ=255)
                                                    tacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACtt                                                                             <  2:81286/136‑1 (MQ=255)
                                                       aGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCAt                                                                          <  2:406119/139‑1 (MQ=255)
                                                         ctctTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATaa                                                                        >  4:71972/1‑139 (MQ=255)
                                                         ctctTCCTTCGCGCTTTCTGTAAAATAAATAAATAAATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTATTTAATGTAATATTCTTTTTGATATCCGTTTTCACTTCATa                                                                         >  5:243376/1‑138 (MQ=255)
                                                            ttCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAAc                                                                       <  6:108278/137‑1 (MQ=255)
                                                            ttCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACtt                                                                     >  4:57383/1‑139 (MQ=255)
                                                              ccTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTc                                                                    >  5:223715/1‑138 (MQ=255)
                                                                         tCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGtttt                                                        >  3:337826/1‑139 (MQ=255)
                                                                                    aataaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATaa                                             <  3:71972/139‑1 (MQ=255)
                                                                                    aataaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATaa                                             <  6:223715/139‑1 (MQ=255)
                                                                                      taaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACg                                           <  7:346063/139‑1 (MQ=255)
                                                                                        aataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGAt                                         <  4:305137/139‑1 (MQ=255)
                                                                                        aataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGAt                                         <  8:137227/139‑1 (MQ=255)
                                                                                            cATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGCTCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCttt                                     >  8:103588/1‑139 (MQ=255)
                                                                                            cATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCttt                                     <  2:60363/139‑1 (MQ=255)
                                                                                               tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                   >  5:71533/1‑138 (MQ=255)
                                                                                               tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                   >  3:60538/1‑138 (MQ=255)
                                                                                               tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                   >  8:169309/1‑138 (MQ=255)
                                                                                               tGCGGCACACGATTAAGTCAAAGGCTTGTTCATAACGATCCGCCAGCAAATTGTGATTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                   >  4:72123/1‑138 (MQ=255)
                                                                                                 cGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                   >  3:98203/1‑136 (MQ=255)
                                                                                                  ggCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                               <  4:337826/139‑1 (MQ=255)
                                                                                                    cacacgATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATAGTGCTTTTTCAATGTATTATTCTTTTTGATTTCCGTTTTCACTTCATACCTACTATTTGCGTTTTGCG‑AAAATAACGATCTTTCACAGAtag                            >  3:276149/1‑137 (MQ=255)
                                                                                                      cacgATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagc                           >  6:25971/1‑136 (MQ=255)
                                                                                                                   aaGTCTTGTTCATAACGATCCGCCAGCAATTTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTATTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTga               >  1:365270/1‑137 (MQ=255)
                                                                                                                   aaGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGCTCTTTCACAGATAGCGGCCCTTCCTGt               >  5:282011/1‑138 (MQ=255)
                                                                                                                         tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc        >  3:65381/1‑139 (MQ=255)
                                                                                                                         tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc        >  8:4063/1‑139 (MQ=255)
                                                                                                                          gTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagcgg                         >  4:98381/1‑116 (MQ=255)
                                                                                                                          gTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagcgg                         <  3:98381/121‑6 (MQ=255)
                                                                                                                           ttCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCAATTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAccg       <  7:405160/138‑2 (MQ=255)
                                                                                                                               tAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt  >  1:321569/1‑139 (MQ=255)
                                                                                                                                aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCTTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACAGCTCCt  >  8:372215/1‑138 (MQ=255)
                                                                                                                                aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt  >  4:296291/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCT  >  CP006881/2198284‑2198549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A