Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F56 I20 R2
|
1958 |
92.1 |
3575798 |
91.1% |
3257551 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
3,970,967 |
G→T |
L192I (CTT→ATT) |
U712_20470 ← |
Sensor histidine kinase WalK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,970,967 | 0 | G | T | 96.4%
| 162.4
/ NA
| 56 | L192I (CTT→ATT) | U712_20470 | Sensor histidine kinase WalK |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (21/33); total (22/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCATCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGC > CP006881/3970834‑3971093
|
tcatcaTGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGcc > 1:425530/1‑138 (MQ=255)
catcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCa < 4:16128/138‑1 (MQ=255)
tcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAAc < 4:209871/139‑1 (MQ=255)
atGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCt > 5:189690/1‑139 (MQ=255)
tCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCtgtg > 7:341656/1‑139 (MQ=255)
tCCATAC‑TTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGc > 3:410524/1‑139 (MQ=255)
cATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGc < 2:232439/138‑1 (MQ=255)
cATAC‑TTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 4:410524/139‑1 (MQ=255)
aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 4:310514/139‑1 (MQ=255)
aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 3:75294/139‑1 (MQ=255)
aTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 5:390499/139‑1 (MQ=255)
aTACTTTTTAACCTTCCTAGAGAAATTCCCTTTCGCAATCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 6:44999/139‑1 (MQ=255)
aCTTTTTAACCTTCCGCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGa < 8:189391/139‑1 (MQ=255)
cTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGa < 2:425530/138‑1 (MQ=255)
tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc > 8:350892/1‑138 (MQ=255)
tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGc < 4:153197/138‑1 (MQ=255)
tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGAAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGcc > 4:142001/1‑139 (MQ=255)
tAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAg > 4:175675/1‑139 (MQ=255)
aCCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTa < 2:157211/139‑1 (MQ=255)
tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt > 2:57489/1‑139 (MQ=255)
tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt > 1:392645/1‑139 (MQ=255)
cTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGTGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgtg < 7:424366/138‑1 (MQ=255)
tCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCAtt < 4:18624/137‑1 (MQ=255)
tCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCAtt < 4:341125/137‑1 (MQ=255)
aaGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa < 2:392645/139‑1 (MQ=255)
gCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGTGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa < 1:397835/137‑1 (MQ=255)
cTCCATCGGCTGGTTTAGCATATCTGAAAGAGGGTGGGTAATGGTTCTTGCAAGAAAACTACCTAGAAGAGCCGTCAAAACAATTGCGAAACTTGGGGCGGTCGCGAGTATCGTGTTGATCGTCTTCATTTGATTaatg < 6:311698/139‑3 (MQ=255)
cTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAg < 8:365670/139‑1 (MQ=255)
ccATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc < 4:111855/139‑1 (MQ=255)
ccATCGCCTGCTTTCGCATATCTCAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc < 4:392742/139‑1 (MQ=255)
cATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGAc < 3:175675/138‑1 (MQ=255)
tCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt < 8:341656/138‑1 (MQ=255)
tCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt < 7:6982/138‑1 (MQ=255)
acagtCTGAAAGCGGGTGTGTAATGGTTCTTTCCAGAAAAATACCGAGAAGAGCCGTCAAAAAAATTGCCAAACCTGTGCCGGACGCCATTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTcgcg < 6:336552/135‑1 (MQ=255)
tCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACaa > 1:46268/1‑139 (MQ=255)
cGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATc < 8:323637/139‑1 (MQ=255)
gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACAt < 4:330757/110‑1 (MQ=255)
gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACAt > 3:330757/1‑110 (MQ=255)
gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATcgcg < 7:230145/139‑1 (MQ=255)
gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATATTCCATACTCGCGACAACGTAGATctg > 5:336552/1‑136 (MQ=255)
gtgggtAATGGTTCTTGCCAGAAAAATACCGAAAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa > 3:294623/1‑103 (MQ=255)
gtgggtAATGGTTCTTGCCAGAAAAATACCGAAAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTa < 4:294623/103‑1 (MQ=255)
cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa > 6:267537/1‑73 (MQ=255)
cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa < 5:267537/73‑1 (MQ=255)
cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt > 3:150761/1‑107 (MQ=255)
cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACt < 4:150761/107‑1 (MQ=255)
cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACACCCTCtt > 2:357804/1‑139 (MQ=255)
tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTgg > 6:241618/1‑139 (MQ=255)
tGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTgg > 3:388660/1‑139 (MQ=255)
ccAGAAACATCCCGTGGATGGCCGTCAAAACAATTGCCAAACGTGGGCGGGACGCCAGTATCGTGTTTTTCGTCTTCATTTTATTATAGACATCTTCCATAGTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtt < 2:422600/139‑1 (MQ=255)
aGAAAAATACCGAGACGAGCCGTCAAAACAATTGCCAAACCTGGGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt < 5:51898/139‑1 (MQ=255)
aGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt < 5:387604/139‑1 (MQ=255)
aaaaaTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCCCAACCTCTTGGtttt > 8:267705/1‑137 (MQ=255)
aaaaaTACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtttt > 7:216029/1‑137 (MQ=255)
agaagaGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCGTCATTTGATTAAAGAAATCTTACATACTCGCGACCACCGAGATCGCGCCCACACCCGCGTTGTTTTCAGTCAATACgg > 2:162561/1‑139 (MQ=255)
ccGTCAAAACAATTTCCAAACCTGTGCCGGACGCCAGTATCGTGTTTATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTATTGGTTTTCAGTCATGACGGGCTTcgc > 4:166156/1‑139 (MQ=255)
|
TCATCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGC > CP006881/3970834‑3971093
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
