Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	113	306.8	9564096	98.5%	9420634	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,130	A→T	intergenic (‑244/‑57)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,130	0	A	T	99.6%	823.4 / NA	240	intergenic (‑244/‑57)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (128/111); total (129/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 245 total aligned reads displayed.

TGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGA  >  CP006881/3722984‑3723276

 gCCTGCTTTCCCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                                                                                                                 <  2:1664603/150‑1 (MQ=255)

 gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                                                                                                                 >  2:1014053/1‑150 (MQ=255)

   cTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATCGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTc                                                                                                                                               <  1:1939870/150‑1 (MQ=255)

    tGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                                              >  2:3080878/1‑150 (MQ=255)

    tGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGAGAAATACTTca                                                                                                                                              >  2:3302071/1‑150 (MQ=255)

        ttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATGGTGAAATACTTCACAat                                                                                                                                          >  1:708325/1‑150 (MQ=255)

         tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                                                         >  2:4686766/1‑150 (MQ=255)

          aCCAGTCACTATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAACTAATTGTGAAATACTTCACAatat                                                                                                                                        >  1:411495/1‑150 (MQ=255)

           ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                                                                       <  2:4299799/150‑1 (MQ=255)

             aGTCACAATCCCGCTTATTCAGATCAAGAATACGCTTTCATCATAAATCATGATAGCCTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAACATCGt                                                                                                                                     >  1:973969/1‑150 (MQ=255)

              gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                                                    <  1:4158815/150‑1 (MQ=255)

               tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                                                                                                   >  1:4053126/1‑150 (MQ=255)

                cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                                                                                  >  1:4393986/1‑150 (MQ=255)

                cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                                                                                  >  1:3776724/1‑150 (MQ=255)

                    aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                              >  2:3594332/1‑150 (MQ=255)

                    aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                              <  2:1911265/150‑1 (MQ=255)

                     tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                                                                                             <  2:2543177/150‑1 (MQ=255)

                        cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                                          >  2:1966343/1‑150 (MQ=255)

                           ttATTCAGATTAAGAATACGCTTTCATCATAAAGCATGACAGGGCTTCCGTCAACTATTTTTTTAGATAAATAGGTTGATATAACATGTAGTCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTc                                                                                                                       <  2:1876169/150‑1 (MQ=255)

                                  gATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAtg                                                                                                                >  1:1105666/1‑149 (MQ=255)

                                   aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAtga                                                                                                               >  2:2282790/1‑148 (MQ=255)

                                    ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaa                                                                                                              >  1:2913323/4‑150 (MQ=255)

                                     taagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaa                                                                                                             >  2:1321097/3‑150 (MQ=255)

                                       agaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                                                                                           >  2:213480/1‑150 (MQ=255)

                                          aTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                                                                        >  2:3723209/1‑150 (MQ=255)

                                           tACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGc                                                                                                       <  1:3033213/150‑1 (MQ=255)

                                           gaCGCTTTCATCATAACTCATGATAGCGTTTTCGACAACTATTTTTTTAGTTAAGTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCCCAACCATGAAAGAAAGc                                                                                                       <  2:11575/149‑1 (MQ=255)

                                            aCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCa                                                                                                      <  2:1691681/150‑1 (MQ=255)

                                                  tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaa                                                                                                <  1:3685308/150‑1 (MQ=255)

                                                    atcatAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGgaaaga                                                                                              >  2:1581946/1‑148 (MQ=255)

                                                    atcatAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                                                                                              >  2:2554397/1‑148 (MQ=255)

                                                      catAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGGTCAATCATGAAAGAAAGCAGGAAAAGACa                                                                                            >  1:399116/1‑150 (MQ=255)

                                                      catAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACa                                                                                            <  1:2787190/150‑1 (MQ=255)

                                                          aaTCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCGATCATGAAAGAAAGCAGGAAAAGACAATgg                                                                                        <  2:4337288/150‑1 (MQ=255)

                                                            tcaTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaa                                                                                      >  2:3111411/3‑150 (MQ=255)

                                                            tcaTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaa                                                                                      <  1:1133084/148‑1 (MQ=255)

                                                               tGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                                                   >  1:2399731/1‑150 (MQ=255)

                                                               tGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                                                   <  2:4359606/150‑1 (MQ=255)

                                                                 aTAGCGTTCCCGTCAACTATTTTTTTAGTAAAATAGTTTGATATATCTTGTAGACAGACATTCGTAAAAAGTCATTGTGAAATACTTCACAATAGCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                                                                                 <  1:1428860/150‑1 (MQ=255)

                                                                   aGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGGCAATGGAAACTCAAg                                                                               >  1:4437369/1‑150 (MQ=255)

                                                                     cGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                                                             <  2:4507812/150‑1 (MQ=255)

                                                                      gTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAt                                                                            <  2:205700/150‑1 (MQ=255)

                                                                       ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACATAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtt                                                                           <  2:927980/150‑1 (MQ=255)

                                                                       ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCGATCATGAAAGAAAGGAGGAAAAGACAATGGAAACTCAAGCAtt                                                                           >  1:4583434/1‑150 (MQ=255)

                                                                           cGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAAAGGAAACTCAAGCATTACaa                                                                       >  1:2565544/1‑150 (MQ=255)

                                                                                actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGt                                                                  >  1:2022190/6‑150 (MQ=255)

                                                                                  tatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTg                                                                >  1:3461783/4‑150 (MQ=255)

                                                                                    tttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa                                                              <  1:2884377/149‑1 (MQ=255)

                                                                                        tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATCCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGt                                                          <  2:4567573/150‑1 (MQ=255)

                                                                                        tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGt                                                          <  1:3289434/150‑1 (MQ=255)

                                                                                         ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTa                                                         <  2:3929900/150‑1 (MQ=255)

                                                                                         ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTa                                                         <  2:3569936/150‑1 (MQ=255)

                                                                                              tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCtt                                                    >  2:2616247/1‑150 (MQ=255)

                                                                                                aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTg                                                  >  1:159260/1‑150 (MQ=255)

                                                                                                aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTg                                                  <  2:226402/150‑1 (MQ=255)

                                                                                                 aTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGa                                                 >  1:4035291/1‑150 (MQ=255)

                                                                                                 aTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGa                                                 >  1:3633962/1‑150 (MQ=255)

                                                                                                  tAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaa                                                <  2:4104140/150‑1 (MQ=255)

                                                                                                  tAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaa                                                <  2:3466414/150‑1 (MQ=255)

                                                                                                  tAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaa                                                <  2:4182828/150‑1 (MQ=255)

                                                                                                  tAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaa                                                >  1:3791811/1‑150 (MQ=255)

                                                                                                    gTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaa                                              <  1:350876/150‑3 (MQ=255)

                                                                                                     tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGGATGCTTTGAaaaa                                             >  1:744750/1‑150 (MQ=255)

                                                                                                      ttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaaaa                                            <  2:331628/150‑1 (MQ=255)

                                                                                                            taacatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAaaaacaaaac                                      <  1:1580802/143‑1 (MQ=255)

                                                                                                            taacatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAAACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAaaaacaaaac                                      <  1:3111411/143‑1 (MQ=255)

                                                                                                              acatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACat                                    >  2:4287892/6‑150 (MQ=255)

                                                                                                              acatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTCTGAAACAACAAAACat                                    >  2:4379694/6‑150 (MQ=255)

                                                                                                                atggaGACAAAAAATCGTAAAAATTAATTGTGAAAAACTTCACAATATCATGCTATACTATGCACAATCATGAAAGAAAGCAGGAAAAAACAATGGGAACTCAAGCATTACAAAGAGTTGAACAGTATGCTTTGAAAAAACAAAACatat                                  >  1:1099844/5‑150 (MQ=255)

                                                                                                                 tgtAGACAGAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAtt                                 <  1:639882/148‑1 (MQ=255)

                                                                                                                 tgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAtt                                 <  1:4549071/148‑1 (MQ=255)

                                                                                                                         aaaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTcaa                         >  1:233273/1‑148 (MQ=255)

                                                                                                                          aaaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAg                        <  2:3776722/150‑1 (MQ=255)

                                                                                                                           aaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGc                       >  1:3175370/1‑150 (MQ=255)

                                                                                                                            aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGGAAAAACAAAACATATTCGCTTCAAGCa                      >  2:3755392/1‑150 (MQ=255)

                                                                                                                            aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCa                      >  2:163636/1‑150 (MQ=255)

                                                                                                                             tccGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaa                     <  2:176200/148‑1 (MQ=255)

                                                                                                                               cGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaaa                   >  2:2907881/1‑150 (MQ=255)

                                                                                                                                  aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc                >  2:2091760/1‑150 (MQ=255)

                                                                                                                                  aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc                <  1:833614/150‑1 (MQ=255)

                                                                                                                                  aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc                >  2:2691440/1‑150 (MQ=255)

                                                                                                                                    aaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGt              <  1:2616247/150‑1 (MQ=255)

                                                                                                                                    aaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGt              >  2:3198547/1‑150 (MQ=255)

                                                                                                                                     aattaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGtt             <  1:1215024/144‑1 (MQ=255)

                                                                                                                                     aattaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGtt             >  2:340749/7‑150 (MQ=255)

                                                                                                                                     aattaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGtt             >  1:1858422/7‑150 (MQ=255)

                                                                                                                                      attaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTa            <  2:2867178/145‑1 (MQ=255)

                                                                                                                                      attaattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTa            >  1:2258623/6‑150 (MQ=255)

                                                                                                                                         aattGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAtgt         >  2:3095089/3‑150 (MQ=255)

                                                                                                                                          attGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTAtgtg        >  2:912257/2‑150 (MQ=255)

                                                                                                                                           ttGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGc       >  2:1217638/1‑150 (MQ=255)

                                                                                                                                            tgtgAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGCTATGTGCt      >  2:185770/1‑150 (MQ=255)

                                                                                                                                             gtgAAATACTCCACAATATCGTGCTATACTATGCTCAACCGCGACAGAAAGCAGGAAAGGACAATGGAAACTCAAGCATTACCACAGGTTGAACAGCATGCTTTGAAAGAACAAAACATATACGCTTCAGGCAAAATCCGTTATGTGCtt     >  2:642011/1‑149 (MQ=255)

                                                                                                                                              tgAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttc    >  1:530359/1‑148 (MQ=255)

                                                                                                                                              tgAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttc    >  2:1443587/1‑148 (MQ=255)

                                                                                                                                              tgAAATACTTCACAAGATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTAATGTGCttc    >  2:3252342/1‑148 (MQ=255)

                                                                                                                                               gAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcg   <  2:4420547/150‑4 (MQ=255)

                                                                                                                                               gAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcg   >  2:537257/1‑147 (MQ=255)

                                                                                                                                                aaataCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcgg  >  1:1888931/1‑146 (MQ=255)

                                                                                                                                                aaataCTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcgg  >  2:3851617/1‑146 (MQ=255)

TGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGA  >  CP006881/3722984‑3723276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A