Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F53 I1 R1
|
117 |
75.6 |
2693952 |
95.6% |
2575418 |
126.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
3,724,623 |
A→T |
I68I (ATT→ATA) |
U712_19200 ← |
SacPA operon antiterminator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,724,623 | 0 | A | T | 98.3%
| 193.6
/ NA
| 60 | I68I (ATT→ATA) | U712_19200 | SacPA operon antiterminator |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (37/22); total (38/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCC > CP006881/3724489‑3724754
|
ttCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATTAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTatat < 7:10954/139‑1 (MQ=255)
ttCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTatat < 1:306433/139‑1 (MQ=255)
cacaAGCAGCCCATTTTGAATCGTTTCGATCCCTAAGTACAAATGGTCGGAACGCGCGATATGAATTTGTTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTTATGATATCCTCAGCTATTTCTATATGctc < 5:312579/138‑1 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCTATCGCAAAGGACAAATGGTCTGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGAGAGCTATACTTAAGCATAGCTGATGATATACTCAGCTAATTCAatattcaaa > 6:237389/1‑134 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctcc > 7:283529/1‑139 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc < 1:83537/138‑1 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc < 2:284375/136‑1 (MQ=255)
cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTAGGAAAGCGCGATATGAAAGTCATCGCTCAGAGGCGCCGCGAGCAATCCATACGCATAGCTGATGATATACACAGCAATTTCTATATGatc > 6:312579/1‑138 (MQ=255)
agcagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCg < 6:136221/139‑1 (MQ=255)
cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc < 3:90795/139‑1 (MQ=255)
agcCCATTTTTAATCCTATCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa < 4:173912/139‑1 (MQ=255)
cccATTTTGAATCCTTTCGATCGCAAAGTAAAAATGGTCGTAAAGAGATATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATCATATCCTCAGCTATTTCTATATGCTCCTCCGGCa < 5:237389/137‑1 (MQ=255)
cccATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgt < 6:110431/139‑1 (MQ=255)
aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa < 6:107176/139‑1 (MQ=255)
tttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCAGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTg > 7:239171/1‑139 (MQ=255)
ttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATTATATACTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGt > 5:281384/1‑139 (MQ=255)
ttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGt > 1:175339/1‑139 (MQ=255)
ttCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATAAGAATGTGATCGCTCAGCGGCGCCGCGCGCTCTCCTTCCCCATAGCTGATGATATACTCATCTATTTCTATATACTCCTCAGGAAGTGTTAGCACGATATGt > 1:334631/1‑139 (MQ=255)
cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGCTGAAGATAACCTCAGCTATATCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGGTTGAAATTTTCATTCtcgtctcg > 8:204255/3‑136 (MQ=255)
cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg > 5:277612/3‑137 (MQ=255)
cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg > 8:316915/3‑137 (MQ=255)
cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGAATAGCTGAAGATATCCTCAGCAATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGTTTTGTTTGAACTTTTAATTAtcgtcgcg > 3:7147/3‑137 (MQ=255)
cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTACTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATATGTTTGAACTTTTCATTCtcgtcgcg > 1:282250/3‑137 (MQ=255)
cgcgATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAAGTTTTCATTCTCGTCGCGCACGAc > 4:228209/1‑138 (MQ=255)
gATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg < 2:254261/139‑1 (MQ=255)
aTGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCGTTTTCTATTTGCTCCTCCGGCAGTGTTTGCAGGATTTGGTTGAACTTTTCATTATCGTCGCGCACGACAAAGCTCtttt > 5:301693/1‑139 (MQ=255)
tgtgATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGGAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCCCGACAAAGATCTTTTc > 2:243461/1‑139 (MQ=255)
aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt > 6:115487/1‑139 (MQ=255)
aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCACTCTCGTCGCGCACGACAAAGATCTTTTCCACt < 3:158287/139‑1 (MQ=255)
aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGGTTGCAGGATTTGTTTGATCTTTTCTTTCTCGTCGCGCACGACAAAGATCTTTTCGACt > 1:312805/1‑139 (MQ=255)
aTCGCTCAGCGGCGCCGCGAGCTCTCCTTACGCATAACTTATGATATAATAATATATTTATATATGCTCATCAGGCAGTGTTTGCAGGATTTGTTTGAAATTTTCATTCTCGTCTCGAACGACAAAGATCTTTTCCACt > 8:267778/1‑139 (MQ=255)
cTCAGCGGCGCCGCGCGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACATTTCATTATCGTAGCGCACGACAAAGCTCTTTAACACTTTAt > 8:257210/1‑139 (MQ=255)
aGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCa < 4:32222/139‑1 (MQ=255)
aGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCa < 1:14891/139‑1 (MQ=255)
gccgcGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGa > 7:329341/1‑139 (MQ=255)
ccgcGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGAt > 3:52872/1‑139 (MQ=255)
ccgcGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGAt < 8:49259/139‑1 (MQ=255)
cgcgcgCTCTCCTTCCGCATAGCTGATGATATCCTCAGGAATTTCTATATGCTACTCCGGCAGTGTTTGCATGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAACATCTTTTCCACGTTATTAATAtggttt > 1:171310/1‑136 (MQ=255)
ccGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt > 4:145899/1‑105 (MQ=255)
ccGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt < 3:145899/105‑1 (MQ=255)
cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt > 4:134554/1‑139 (MQ=255)
cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTATGCAGGATGTGTTTGGAGTTTTCATTCTCGTCGCGCACGACAAAGAGCTTTTGCACTTTATTAGTAGGGATGATAGCATGTTTCttgtt > 4:269164/1‑139 (MQ=255)
tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg > 1:220669/1‑50 (MQ=255)
tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg < 2:220669/50‑1 (MQ=255)
aGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttttt > 8:277486/1‑138 (MQ=255)
atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTTCAGGATTTGTGTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATAATATTTCTTTTTTTTAAAAg > 1:77368/1‑139 (MQ=255)
atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAg > 6:292404/1‑139 (MQ=255)
atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAg > 4:207376/1‑139 (MQ=255)
atgatATACTCAGCTATTTCTATATGCTCCTCCGTCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATATTTTAGACTTTATTAATAGGGATGAGATCATTTTTATTTTTTTGaa > 7:301677/1‑136 (MQ=255)
ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAATAGCGATTcc > 2:297908/1‑139 (MQ=255)
ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc > 7:305186/1‑139 (MQ=255)
cTCAGCTATTTCTATCTGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGAAAAAGTTCTTTTCCAGATTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc < 2:334631/138‑1 (MQ=255)
aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCa > 6:207265/1‑95 (MQ=255)
aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCa < 5:207265/95‑1 (MQ=255)
aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt > 6:84414/1‑139 (MQ=255)
aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt > 5:195560/1‑139 (MQ=255)
aGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGAGCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGt < 8:206326/139‑1 (MQ=255)
cTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt > 7:75002/1‑81 (MQ=255)
cTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCtttt < 8:75002/81‑1 (MQ=255)
cTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTcc > 8:306387/1‑139 (MQ=255)
|
TTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCC > CP006881/3724489‑3724754
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
