Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F54 I1 R1	119	63.3	2848040	95.5%	2719878	127.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,724,623	A→T	I68I (ATT→ATA)	U712_19200 ←	SacPA operon antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,623	0	A	T	98.3%	186.9 / NA	58	I68I (ATT→ATA)	U712_19200	SacPA operon antiterminator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (28/29); minor base G (1/0); total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGG  >  CP006881/3724494‑3724751

cacaAGCAGCCTATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                                                                                                            <  2:271281/136‑1 (MQ=255)

cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                                                                                                          <  8:98102/138‑1 (MQ=255)

cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                                                                                                          <  7:177347/138‑1 (MQ=255)

cacaAGAAGCCCATTTTGAATCCTTTCGATCGGAAAGGACAAATGGTCTGATAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCCAGATCTCCTTCCGCATAGCTGATGATAACCTCAGCTATATCTATATGctca                                                                                                                         >  6:146948/1‑138 (MQ=255)

     gcagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCgg                                                                                                                    >  1:276956/1‑139 (MQ=255)

        gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAg                                                                                                                 <  8:352945/139‑1 (MQ=255)

        gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAg                                                                                                                 <  3:310138/139‑1 (MQ=255)

        gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCGCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAg                                                                                                                 >  3:75761/1‑139 (MQ=255)

             ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa                                                                                                                  <  1:143873/133‑1 (MQ=255)

             ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa                                                                                                                  >  2:143873/1‑133 (MQ=255)

             ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGttt                                                                                                            >  4:47430/1‑139 (MQ=255)

               ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGGTATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg                                                                                                           <  2:41986/138‑1 (MQ=255)

                   aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCGCTCCTGCCGCATAGAAGAAGATATACTCAGCGATTGATTAATGCTCCTCCGGCACTGGTTGCAgg                                                                                                       >  2:303855/1‑138 (MQ=255)

                        ttCGATCGCAAAGGACAAATGGTCGGGAAGCGCGATATGAATGTGATCGCTCCGCGGCGCCGCGCGCTCTCCGTCCGCATAGCTGATGATCTCCTCAGCTATTACTATATGCTCCTCCGGCAGGGGTTGCAGGAttttt                                                                                                 >  3:189004/1‑137 (MQ=255)

                            aTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtttga                                                                                             <  7:129231/139‑3 (MQ=255)

                             tCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTgaa                                                                                            <  8:203423/139‑1 (MQ=255)

                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATTCTCCTACGGCAGTGTTTGCAGGATTTGTTTTAACTTTTCATTCtcgtagcg                                                                          >  6:65661/3‑135 (MQ=255)

                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtcgcg                                                                          >  4:236485/3‑137 (MQ=255)

                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtagtcgcg                                                                          >  3:200970/3‑137 (MQ=255)

                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTACGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCtcgtctcg                                                                          >  3:281401/3‑136 (MQ=255)

                                               cggAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCAGATGATATACTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGGAGGATTTGTTTGAACTTTACTTTAtcgtcgcg                                                                          >  2:323420/3‑137 (MQ=255)

                                                 gAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                                                        <  1:242839/139‑1 (MQ=255)

                                                 gAAAGCGCGATATGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCa                                                                        <  3:61544/139‑1 (MQ=255)

                                                        cgATATGAATGTGATCGCTCAGCGGCGCCGCGAGCGCTCCTTCCGCATAGATGATGATATCCTCAGCTATTTCTATATTAAACTCAGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTATCGTAGCGCACTCCaaa                                                                 >  4:50964/1‑139 (MQ=255)

                                                         gATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg                                                                <  3:236485/139‑1 (MQ=255)

                                                         gATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg                                                                <  5:139307/139‑1 (MQ=255)

                                                         gATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg                                                                <  3:319146/139‑1 (MQ=255)

                                                         catatGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAg                                                                <  1:323420/138‑1 (MQ=255)

                                                                     gtCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  5:136890/138‑1 (MQ=255)

                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    >  3:297560/1‑139 (MQ=255)

                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  3:47430/139‑1 (MQ=255)

                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  3:134483/139‑1 (MQ=255)

                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  2:181742/139‑1 (MQ=255)

                                                                     aTCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  4:297560/139‑1 (MQ=255)

                                                                     aTCGCGCAGCTGCGCCGCTAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  5:130067/139‑1 (MQ=255)

                                                                         cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                      >  8:89793/1‑85 (MQ=255)

                                                                         cTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa                                                                                                      <  7:89793/85‑1 (MQ=255)

                                                                         cTCAGCGGAGCCGAGAGCTCTCCTACCGCATAGCTGCTGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTAt                                                <  6:23507/139‑1 (MQ=255)

                                                                                 cgccgcGAGCTCACCTTACGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTAATTCTAGTCGCGCACGACAAAGATCTTTTCAACTTTATTCATAgga                                        >  6:136890/1‑138 (MQ=255)

                                                                                   ccgcGCGCTCGCCGTCCGCATAGCTGATGATATCCTCAGCAATTTCGATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGCCAAAGCTCTTTTCCACGTTATTGATAGGGAt                                      >  3:233276/1‑139 (MQ=255)

                                                                                                     cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGCTCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt                    >  5:209707/1‑139 (MQ=255)

                                                                                                     cATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttt                    >  2:312730/1‑139 (MQ=255)

                                                                                                       tAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAATGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCttttttt                  >  2:150765/1‑139 (MQ=255)

                                                                                                             atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAg            <  8:260838/139‑1 (MQ=255)

                                                                                                             atgatATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAg            >  1:233095/1‑139 (MQ=255)

                                                                                                                atatCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCg          <  2:233095/138‑1 (MQ=255)

                                                                                                                  atCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACtttt                                                                                       >  7:296666/1‑59 (MQ=255)

                                                                                                                  atCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACtttt                                                                                       <  8:296666/59‑1 (MQ=255)

                                                                                                                  atCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTAGCAGGATGTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCATTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGAtt       >  6:327533/1‑139 (MQ=255)

                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    >  4:24526/1‑92 (MQ=255)

                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  3:24526/92‑1 (MQ=255)

                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc     >  1:3200/1‑139 (MQ=255)

                                                                                                                    ccTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc     >  2:21865/1‑139 (MQ=255)

                                                                                                                     cTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTcc     <  5:65661/138‑1 (MQ=255)

                                                                                                                       cAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    >  2:333329/1‑89 (MQ=255)

                                                                                                                       cAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACt                                                    <  1:333329/89‑1 (MQ=255)

                                                                                                                       cAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCgg  >  1:104459/1‑139 (MQ=255)

                                                                                                                       cAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCAgg  >  1:326475/1‑139 (MQ=255)

CACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGG  >  CP006881/3724494‑3724751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A